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1.
Autophagy ; 19(5): 1611-1613, 2023 05.
Article in English | MEDLINE | ID: mdl-36760098

ABSTRACT

Light is essential for plant growth, but excessive light energy produces reactive oxygen species (ROS), which can seriously damage cells. Mutants defective in ATG (autophagy related) genes show light intensity-dependent leaf damage and ROS accumulation. We found that autophagy is one of the crucial systems in protecting plants from ROS-induced damage by removing oxidative peroxisomes. Damaged peroxisomes are targeted by the PtdIns3P marker and specifically engulfed by phagophores labeled by ATG18a-GFP. Under high-intensity light, huge peroxisome aggregates are induced and captured by vacuolar membranes. Research provides a deeper understanding of plant stress response to light irradiation.


Subject(s)
Autophagy , Macroautophagy , Autophagy/physiology , Reactive Oxygen Species/metabolism , Oxidative Stress , Plants , Peroxisomes/metabolism
2.
Nat Commun ; 13(1): 7493, 2022 12 05.
Article in English | MEDLINE | ID: mdl-36470866

ABSTRACT

Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation.


Subject(s)
Macroautophagy , Peroxisomes , Reactive Oxygen Species/metabolism , Peroxisomes/metabolism , Autophagy/physiology , Plant Leaves/metabolism
3.
J Biol Chem ; 298(6): 102038, 2022 06.
Article in English | MEDLINE | ID: mdl-35595097

ABSTRACT

Protein transport to peroxisomes requires various proteins, such as receptors in the cytosol and components of the transport machinery on peroxisomal membranes. The Arabidopsis apem (aberrant peroxisome morphology) mutant apem7 shows decreased efficiency of peroxisome targeting signal 1-dependent protein transport to peroxisomes. In apem7 mutants, peroxisome targeting signal 2-dependent protein transport is also disturbed, and plant growth is repressed. The APEM7 gene encodes a protein homologous to peroxin 4 (PEX4), which belongs to the ubiquitin-conjugating (UBC) protein family; however, the UBC activity of Arabidopsis PEX4 remains to be investigated. Here, we show using electron microscopy and immunoblot analysis using specific PEX4 antibodies and in vitro transcription/translation assay that PEX4 localizes to peroxisomal membranes and possesses UBC activity. We found that the substitution of proline with leucine by apem7 mutation alters ubiquitination of PEX4. Furthermore, substitution of the active-site cysteine residue at position 90 in PEX4, which was predicted to be a ubiquitin-conjugation site, with alanine did not restore the apem7 phenotype. Taken together, these findings indicate that abnormal ubiquitination in the apem7 mutant alters ubiquitin signaling during the process of protein transport, suggesting that the UBC activity of PEX4 is indispensable for efficient protein transport to peroxisomes.


Subject(s)
Arabidopsis Proteins , Arabidopsis , Peroxins , Peroxisomes , Ubiquitin-Conjugating Enzymes , Arabidopsis/genetics , Arabidopsis/metabolism , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Mutation , Peroxins/genetics , Peroxins/metabolism , Peroxisomes/metabolism , Protein Transport , Ubiquitin-Conjugating Enzymes/genetics , Ubiquitin-Conjugating Enzymes/metabolism , Ubiquitins/metabolism
4.
Jpn J Clin Oncol ; 49(11): 1016-1023, 2019 Dec 18.
Article in English | MEDLINE | ID: mdl-31665343

ABSTRACT

OBJECTIVE: This surveillance study was conducted to verify the post-market safety and effectiveness of bevacizumab, which was approved in Japan in 2013 for the treatment of patients with newly diagnosed and or recurrent malignant glioma. METHODS: This was a prospective, observational, multicenter post-marketing surveillance study. Patients with newly diagnosed or recurrent malignant glioma scheduled for bevacizumab treatment were enrolled. The incidence and severity of adverse drug reactions were calculated. The effectiveness of bevacizumab was assessed by the 1-year survival rate and the overall survival rate. RESULTS: The safety analysis set and the effectiveness analysis set each comprised 258 of the 268 enrolled patients: tumours were newly diagnosed in 80 patients (31%) and recurrent in 178 patients (68.9%). The incidence of grade ≥ 3 adverse drug reactions was 15.1%. Adverse drug reactions of special interest included 14 cerebral bleeding events and 11 infections. Of the 80 patients with newly diagnosed malignant glioma, 44 (55%) were alive throughout the 18-month observation period. The 1-year survival rate for patients with newly diagnosed glioblastoma was 78%. Median overall survival was not calculated, but 51.2% of patients were alive at the last date of observation of the last observed patient. In patients with recurrent glioblastoma, the 1-year survival rate was 38.9%, and the median overall survival was 10.2 months. CONCLUSIONS: The results suggest no new safety concerns, and the effectiveness might be similar to previously reported data in clinical trials. Therefore, bevacizumab is considered as one of the treatment options for patients with malignant glioma in real-world clinical practice.


Subject(s)
Angiogenesis Inhibitors/therapeutic use , Bevacizumab/adverse effects , Bevacizumab/therapeutic use , Brain Neoplasms/drug therapy , Glioblastoma/drug therapy , Adolescent , Adult , Aged , Aged, 80 and over , Angiogenesis Inhibitors/adverse effects , Antibodies, Monoclonal, Humanized/adverse effects , Antibodies, Monoclonal, Humanized/therapeutic use , Brain Neoplasms/pathology , Child , Child, Preschool , Female , Glioblastoma/pathology , Humans , Japan , Male , Middle Aged , Neoplasm Recurrence, Local/pathology , Neoplasm Recurrence, Local/prevention & control , Product Surveillance, Postmarketing , Prospective Studies , Survival Rate , Young Adult
5.
J Integr Plant Biol ; 61(7): 836-852, 2019 Jul.
Article in English | MEDLINE | ID: mdl-30916439

ABSTRACT

The dynamic behavior of organelles is essential for plant survival under various environmental conditions. Plant organelles, with various functions, migrate along actin filaments and contact other types of organelles, leading to physical interactions at a specific site called the membrane contact site. Recent studies have revealed the importance of physical interactions in maintaining efficient metabolite flow between organelles. In this review, we first summarize peroxisome function under different environmental conditions and growth stages to understand organelle interactions. We then discuss current knowledge regarding the interactions between peroxisome and other organelles, i.e., the oil bodies, chloroplast, and mitochondria from the perspective of metabolic and physiological regulation, with reference to various organelle interactions and techniques for estimating organelle interactions occurring in plant cells.


Subject(s)
Chloroplasts/metabolism , Organelles/metabolism , Peroxisomes/metabolism , Mitochondria/metabolism , Plant Cells/metabolism
6.
J Phycol ; 55(1): 118-133, 2019 02.
Article in English | MEDLINE | ID: mdl-30304548

ABSTRACT

Nanoparticles have unique properties that make them attractive for use in industrial and medical technology industries but can also be harmful to living organisms, making an understanding of their molecular mechanisms of action essential. We examined the effect of three different sized poly(isobutyl-cyanoacrylate) nanoparticles (iBCA-NPs) on the unicellular green alga Chlamydomonas reinhardtii. We found that exposure to iBCA-NPs immediately caused C. reinhardtii to display abnormal swimming behaviors. Furthermore, after one hour, most of the cells had stopped swimming and 10%-30% of cells were stained with trypan blue, suggesting that these cells had severely impaired plasma membranes. Observation of the cyto-ultrastructure showed that the cell walls had been severely damaged and that many iBCA-NPs were located in the space between the cell wall and plasma membrane, as well as inside the cytosol in some cases. A comparison of three strains of C. reinhardtii with different cell wall conditions further showed that the cell mortality ratio increased more rapidly in the absence of a cell wall. Interestingly, cell mortality over time was essentially identical regardless of iBCA-NP size if the total surface area was the same. Furthermore, direct observation of the trails of iBCA-NPs indicated that the first trigger was their contact with the cell wall, which is most likely accompanied by the inactivation or removal of adsorbed proteins from the cell wall surface. Cell mortality was accompanied by the overproduction of reactive oxygen species, which was detected more readily in cells grown under constant light rather than in the dark.


Subject(s)
Chlamydomonas reinhardtii , Nanoparticles , Acrylic Resins , Chlorophyceae , Color
7.
J Biol Chem ; 291(38): 19734-45, 2016 09 16.
Article in English | MEDLINE | ID: mdl-27466365

ABSTRACT

Physical interaction between organelles is a flexible event and essential for cells to adapt rapidly to environmental stimuli. Germinating plants utilize oil bodies and peroxisomes to mobilize storage lipids for the generation of sucrose as the main energy source. Although membrane interaction between oil bodies and peroxisomes has been widely observed, its underlying molecular mechanism is largely unknown. Here we present genetic evidence for control of the physical interaction between oil bodies and peroxisomes. We identified alleles of the sdp1 mutant altered in oil body morphology. This mutant accumulates bigger and more oil body aggregates compared with the wild type and showed defects in lipid mobilization during germination. SUGAR DEPENDENT 1 (SDP1) encodes major triacylglycerol lipase in Arabidopsis Interestingly, sdp1 seedlings show enhanced physical interaction between oil bodies and peroxisomes compared with the wild type, whereas exogenous sucrose supplementation greatly suppresses the interaction. The same phenomenon occurs in the peroxisomal defective 1 (ped1) mutant, defective in lipid mobilization because of impaired peroxisomal ß-oxidation, indicating that sucrose production is a key factor for oil body-peroxisomal dissociation. Peroxisomal dissociation and subsequent release from oil bodies is dependent on actin filaments. We also show that a peroxisomal ATP binding cassette transporter, PED3, is the potential anchor protein to the membranes of these organelles. Our results provide novel components linking lipid metabolism and oil body-peroxisome interaction whereby sucrose may act as a negative signal for the interaction of oil bodies and peroxisomes to fine-tune lipolysis.


Subject(s)
Arabidopsis/physiology , Germination/physiology , Lipid Droplets/metabolism , Lipid Metabolism/physiology , Peroxisomes/metabolism , Sucrose/metabolism , ATP-Binding Cassette Transporters/genetics , ATP-Binding Cassette Transporters/metabolism , Adenosine Triphosphatases , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Carboxylic Ester Hydrolases/genetics , Carboxylic Ester Hydrolases/metabolism , Peroxisomes/genetics
8.
J Plant Res ; 128(1): 177-85, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25413007

ABSTRACT

We cultured Chlamydomonas reinhardtii cells in a minimal culture medium supplemented with various concentrations of acetate, fatty acids, ethanol, fatty alcohols, or sucrose. The presence of acetate (0.5 or 1.0%, w/v) was advantageous for cell growth. To determine whether peroxisomes are involved in fatty acid and fatty alcohol metabolism, we investigated the dynamics of peroxisomes, including changes in their number and size, in the presence of acetate, ethanol, and sucrose. The total volume of peroxisomes increased when cells were grown with acetate, but did not change when cells were grown with ethanol or sucrose. We analyzed cell growth on minimal culture medium supplemented with various fatty acids (carbon chain length ranging from one to ten) to investigate which fatty acids are metabolized by C. reinhardtii. Among them, acetate caused the greatest increase in growth when added to minimal culture media. We analyzed the transcript levels of genes encoding putative glyoxysomal enzymes. The transcript levels of genes encoding malate synthase, malate dehydrogenase, isocitrate lyase, and citrate synthase increased when Chlamydomonas cells were grown on minimal culture medium supplemented with acetate. Our results suggest that Chlamydomonas peroxisomes are involved in acetate metabolism via the glyoxylate cycle.


Subject(s)
Acetates/pharmacology , Chlamydomonas/enzymology , Chlamydomonas/genetics , Gene Expression Regulation, Plant/drug effects , Glyoxysomes/enzymology , Peroxisomes/metabolism , Cell Proliferation/drug effects , Cells, Cultured , Chlamydomonas/cytology , Chlamydomonas/ultrastructure , Culture Media/pharmacology , Genes, Plant , Glyoxysomes/drug effects , Glyoxysomes/genetics , Microscopy, Fluorescence , Peroxisomes/drug effects , Peroxisomes/ultrastructure , RNA, Messenger/genetics , RNA, Messenger/metabolism
9.
Rinsho Shinkeigaku ; 53(6): 430-8, 2013.
Article in Japanese | MEDLINE | ID: mdl-23782820

ABSTRACT

Dropped head syndrome is seen in various diseases. We investigated its pathophysiological mechanisms with physical and radiological examination, surface EMG and responses to physiotherapy. Subjects had dropped head as a complaint, but their primary diagnoses were various. We investigated 16 cases: 5 cases of Parkinson disease, 5 cases of multiple system atrophy predominant parkinsonism, 3 cases of cervical spondylosis and 3 cases with other diagnoses. We found that patients had common findings such as bulging of cervical muscles, and tonic EMG activities mainly in the extensors in the sitting and standing position, but in the flexors of the neck only in the supine position. Of the 16 cases, 14 were treated with physiotherapy to improve the alignment of the pelvis and whole vertebral column; 6 of the 14 cases (63%) showed remarkable improvement. We conclude that the primary reason of dropped head syndrome is unknown in Parkinson disease and cervical spondylosis, but also that many of the patients have secondary changes in alignment of the skeletomuscular system which could be treated with physiotherapy.


Subject(s)
Muscle Weakness/physiopathology , Neck Muscles/physiopathology , Aged , Aged, 80 and over , Electromyography , Female , Humans , Male , Muscle Weakness/etiology , Muscle Weakness/therapy , Physical Therapy Modalities , Syndrome
10.
BMJ Case Rep ; 20122012 Oct 06.
Article in English | MEDLINE | ID: mdl-23045444

ABSTRACT

We analysed the effect of vibration stimulation (VS) on dysbasia of neuromyelitis optica (NMO). The patient was a 36-year-old woman who was diagnosed with NMO and had difficulties in walking. VS was applied to the lower limb muscles on the left, more spastic, side with an ordinary vibrator. The performance of standing up and walking improved with VS. Even with improved performance after VS, the amount of surface EMG of the lower limbs did not increase as a whole, but the EMG patterns among the lower leg muscles changed remarkably. VS produced reciprocity within antagonistic muscles. Variability of EMG amplitudes decreased remarkably during the walking cycle, not only on the vibrated side, but also on the non-vibrated side. The effect lasted longer than several dozen minutes after the cessation of VS. We conjectured that central pattern generator (CPG) and neuronal plasticity were the result of VS.


Subject(s)
Mobility Limitation , Muscle, Skeletal/physiology , Neuromyelitis Optica/physiopathology , Neuronal Plasticity/physiology , Paraplegia/physiopathology , Vibration , Walking/physiology , Adult , Central Pattern Generators/physiology , Electromyography , Female , Humans , Leg/physiology , Muscle Spasticity/physiopathology , Muscle Spasticity/therapy , Neuromyelitis Optica/complications , Neuromyelitis Optica/therapy , Paraplegia/therapy
11.
J Plant Res ; 125(4): 579-86, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22205201

ABSTRACT

In Chlorophycean algal cells, these organelles are generally called microbodies because they lack the enzymes found in the peroxisomes of higher plants. Microbodies in some algae contain fewer enzymes than the peroxisomes of higher plants, and some unicellular green algae in Chlorophyceae such as Chlamydomonas reinhardtii do not possess catalase, an enzyme commonly found in peroxisomes. Thus, whether microbodies in Chlorophycean algae are similar to the peroxisomes of higher plants, and whether they use a similar transport mechanism for the peroxisomal targeting signal (PTS), remain unclear. To determine whether the PTS is present in the microbodies of Chlorophycean algae, and to visualize the microbodies in Chlamydomonas cells, we examined the sub-cellular localization of green fluorescent proteins (GFP) fused to several PTS-like sequences. We detected GFP compartments that were spherical with a diameter of 0.3-1.0 µm in transgenic Chlamydomonas. Comparative analysis of the character of GFP-compartments observed by fluorescence microscopy and that of microbodies by electron microscopy indicated that the compartments were one and the same. The result also showed that the microbodies in Chlorophycean cells have a similar transport mechanism to that of peroxisomes of higher plants.


Subject(s)
Chlamydomonas reinhardtii/ultrastructure , Microbodies/ultrastructure , Biological Transport , Chlamydomonas reinhardtii/chemistry , Chlamydomonas reinhardtii/cytology , Chlamydomonas reinhardtii/metabolism , Green Fluorescent Proteins/analysis , Microbodies/chemistry , Microbodies/metabolism , Microscopy, Fluorescence , Peroxisomes/chemistry , Peroxisomes/metabolism , Peroxisomes/ultrastructure , Plants, Genetically Modified , Signal Transduction
12.
J Gastroenterol ; 45(12): 1235-40, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20635100

ABSTRACT

BACKGROUND: Insufflation with carbon dioxide (CO(2)) in colonoscopy has not been widely adopted and, consequently, limited data are available on insufflated gas volume and blood pCO(2). The aim of this study was to compare CO(2) and air as an insufflation agent in patients undergoing colonoscopy without sedation in terms of insufflated gas volume, pCO(2), pain and examination time. METHODS: This was a randomized, double-blind, control trial. Consecutive patients presenting for colonoscopy, excluding those with lung or malignant disease, were randomized into two groups: insufflation with air or with CO(2), respectively. Insufflated gas volume, pain, pCO(2) and examination time were assessed. RESULTS: The study cohort comprised 120 patients (66 randomized to CO(2) group). No significant difference in insufflated gas volumes was found between the CO(2) and air groups. The mean pCO(2) measured before, during (the peak value) and 30 min after colonoscopy were 40, 43 and 40 mmHg, respectively, in both groups. The pain scores in the air group were significantly greater than those in the CO(2) group until 3 h after the examination. There was a significant faster cecal intubation time and a trend toward shorter examination time in the CO(2) group. CONCLUSIONS: The CO(2) gas volume used in our study (14.0 L) was much greater than that reported by others (8.3 L), but the pCO(2) values were still within the normal reference range, indicating the safety of CO(2) insufflation over a greater range of CO(2) gas volume. Among our patients, CO(2) insufflation was associated with relatively less pain and a shorter examination time. Based on our results, we recommend that CO(2) become the standard gas for insufflation in patients undergoing colonoscopy without sedation.


Subject(s)
Abdominal Pain/etiology , Carbon Dioxide/administration & dosage , Colonoscopy/methods , Abdominal Pain/prevention & control , Aged , Air , Blood Gas Analysis , Carbon Dioxide/metabolism , Double-Blind Method , Female , Humans , Insufflation/methods , Male , Middle Aged , Time Factors
13.
Plant Cell ; 21(11): 3672-85, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19933201

ABSTRACT

The endoplasmic reticulum (ER) is composed of tubules, sheets, and three-way junctions, resulting in a highly conserved polygonal network in all eukaryotes. The molecular mechanisms responsible for the organization of these structures are obscure. To identify novel factors responsible for ER morphology, we employed a forward genetic approach using a transgenic Arabidopsis thaliana plant (GFP-h) with fluorescently labeled ER. We isolated two mutants with defects in ER morphology and designated them endoplasmic reticulum morphology1 (ermo1) and ermo2. The cells of both mutants developed a number of ER-derived spherical bodies, approximately 1 microm in diameter, in addition to the typical polygonal network of ER. The spherical bodies were distributed throughout the ermo1 cells, while they formed a large aggregate in ermo2 cells. We identified the responsible gene for ermo1 to be GNOM-LIKE1 (GNL1) and the gene for ermo2 to be SEC24a. Homologs of both GNL1 and SEC24a are involved in membrane trafficking between the ER and Golgi in yeast and animal cells. Our findings, however, suggest that GNL1/ERMO1 and SEC24a/ERMO2 have a novel function in ER morphology in higher plants.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Endoplasmic Reticulum/ultrastructure , Guanine Nucleotide Exchange Factors/metabolism , Vesicular Transport Proteins/metabolism , Arabidopsis/genetics , Arabidopsis/ultrastructure , Arabidopsis Proteins/biosynthesis , Arabidopsis Proteins/genetics , Endoplasmic Reticulum/genetics , Endoplasmic Reticulum/metabolism , Guanine Nucleotide Exchange Factors/genetics , Intracellular Membranes/metabolism , Intracellular Membranes/ultrastructure , Plant Proteins/biosynthesis , Plant Proteins/genetics , Protein Transport/physiology , Vesicular Transport Proteins/genetics
14.
Protoplasma ; 235(1-4): 57-66, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19214701

ABSTRACT

Peroxisomal enzymatic proteins contain targeting signals (PTS) to enable their import into peroxisomes. These targeting signals have been identified as PTS1 and PTS2 in mammalian, yeast, and higher plant cells; however, no PTS2-like amino acid sequences have been observed in enzymes from the genome database of Cyanidiochyzon merolae (Bangiophyceae), a primitive red algae. In studies on the evolution of PTS, it is important to know when their sequences came to be the peroxisomal targeting signals for all living organisms. To this end, we identified a number of genes in the genome database of the green algae Chlamydomonas reinhardtii, which contains amino acid sequences similar to those found in plant PTS. In order to determine whether these sequences function as PTS in green algae, we expressed modified green fluorescent proteins (GFP) fused to these putative PTS peptides under the cauliflower mosaic virus 35S promoter. To confirm whether granular structures containing GFP-PTS fusion proteins accumulated in the peroxisomes of Closterium ehrenbergii, we observed these cells after the peroxisomes were stained with 3, 3'-diaminobenzidine. Our results confirm that the GFP-PTS fusion proteins indeed accumulated in the peroxisomes of these green algae. These findings suggest that the peroxisomal transport system for PTS1 and PTS2 is conserved in green algal cells and that our fusion proteins can be used to visualize peroxisomes in live cells.


Subject(s)
Chlorophyta/metabolism , Peroxisomes/metabolism , Receptors, Cytoplasmic and Nuclear/metabolism , Recombinant Fusion Proteins/metabolism , Chlorophyta/ultrastructure , Green Fluorescent Proteins/metabolism , Microscopy, Electron, Transmission , Mitochondria/metabolism , Mitochondria/ultrastructure , Organisms, Genetically Modified/genetics , Organisms, Genetically Modified/metabolism , Peroxisomal Targeting Signal 2 Receptor , Peroxisome-Targeting Signal 1 Receptor , Peroxisomes/ultrastructure , Signal Transduction
15.
Plant Cell Physiol ; 47(1): 164-75, 2006 Jan.
Article in English | MEDLINE | ID: mdl-16306062

ABSTRACT

Myrosin cells in Capparales plants are idioblasts that accumulate thioglucoside glucohydrolase (TGG, also called myrosinase), which hydrolyzes glucosinolates to produce toxic compounds for repelling pests. Here, we show that AtVAM3 is involved in development of myrosin cells. It has been shown that yeast VAM3 is a Q(a)-SNARE that is involved in vesicle transport of vacuolar proteins and vacuolar assembly. We found that two Arabidopsis atvam3 alleles, atvam3-3 and atvam3-4/ssm, accumulate large amounts of TGG1 and TGG2 that are enzymatically active. An immunogold analysis revealed that TGGs were specifically localized in the vacuole of myrosin cells in atvam3 mutants. This result indicates that TGGs are normally transported to vacuoles in these mutants and that AtVAM3 is not essential for vacuolar transport of the proteins. We developed a staining method with Coomassie brilliant blue that detects myrosin cells in whole leaves by their high TGG content. This method showed that atvam3 leaves have a larger number of myrosin cells than do wild-type leaves. Myrosin cells were scattered along leaf veins in wild-type leaves, while they were abnormally distributed in atvam3 leaves. The mutants developed a network of myrosin cells throughout the leaves: myrosin cells were not only distributed continuously along leaf veins, but were also observed independent of leaf veins. The excess of myrosin cells in atvam3 mutants might be responsible for the abnormal abundance of TGGs and the reduction of elongation of inflorescence stems and leaves in these mutants. Our results suggest that AtVAM3 has a plant-specific function in development of myrosin cells.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/cytology , Arabidopsis/metabolism , Qa-SNARE Proteins/metabolism , Alleles , Amino Acid Sequence , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Base Sequence , DNA, Plant/genetics , Gene Deletion , Genes, Plant , Glycoside Hydrolases/metabolism , Mutation , Phenotype , Plant Leaves/cytology , Plant Leaves/metabolism , Qa-SNARE Proteins/genetics , Vacuoles/metabolism
16.
Plant Cell Physiol ; 44(7): 661-6, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12881493

ABSTRACT

Plant cells develop various endoplasmic reticulum (ER)-derived structures with specific functions. The ER body, a novel ER-derived compartment in Arabidopsis, is a spindle-shaped structure (approximately 10 microm long and approximately 1 microm wide) that is surrounded by ribosomes. Similar structures were found in many Brassicaceae plants in the 1960s and 1970s, but their main components and biological functions have remained unknown. ER bodies can be visualized in transgenic Arabidopsis expressing the green fluorescent protein with an ER-retention signal. A large number of ER bodies are observed in cotyledons, hypocotyls and roots of seedlings, but very few are observed in rosette leaves. Recently nai1, a mutant that does not develop ER bodies in whole seedlings, was isolated. Analysis of the nai1 mutant reveals that a beta-glucosidase, called PYK10, is the main component of ER bodies. The putative biological function of PYK10 and the inducibility of ER bodies in rosette leaves by wound stress suggest that the ER body functions in the defense against herbivores.


Subject(s)
Arabidopsis/physiology , Endoplasmic Reticulum/physiology , Arabidopsis/genetics , Arabidopsis/ultrastructure , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Endoplasmic Reticulum/ultrastructure , Green Fluorescent Proteins , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Microscopy, Electron , Mutation , Plant Structures/genetics , Plant Structures/metabolism , Plant Structures/ultrastructure , Plants, Genetically Modified , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , beta-Glucosidase/genetics , beta-Glucosidase/metabolism
17.
Plant Physiol ; 130(4): 1807-14, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12481064

ABSTRACT

The endoplasmic reticulum (ER) body is a characteristic structure derived from ER and is referred to as a proteinase-sorting system that assists the plant cell under various stress conditions. Fluorescent ER bodies were observed in transgenic plants of Arabidopsis expressing green fluorescent protein fused with an ER retention signal. ER bodies were widely distributed in the epidermal cells of whole seedlings. In contrast, rosette leaves had no ER bodies. We found that wound stress induced the formation of many ER bodies in rosette leaves. ER bodies were also induced by treatment with methyl jasmonate (MeJA), a plant hormone involved in the defense against wounding and chewing by insects. The induction of ER bodies was suppressed by ethylene. An electron microscopic analysis showed that typical ER bodies were induced in the non-transgenic rosette leaves treated with MeJA. An experiment using coi1 and etr1-4 mutant plants showed that the induction of ER bodies was strictly coupled with the signal transduction of MeJA and ethylene. These results suggested that the formation of ER bodies is a novel and unique type of endomembrane system in the response of plant cells to environmental stresses. It is possible that the biological function of ER bodies is related to defense systems in higher plants.


Subject(s)
Adaptation, Physiological/physiology , Arabidopsis/physiology , Endoplasmic Reticulum/physiology , Acetates/pharmacology , Adaptation, Physiological/drug effects , Arabidopsis/drug effects , Arabidopsis/genetics , Cyclopentanes/pharmacology , Endoplasmic Reticulum/drug effects , Endoplasmic Reticulum/ultrastructure , Ethylenes/pharmacology , Gene Expression Regulation, Plant , Green Fluorescent Proteins , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Microscopy, Electron , Mutation , Oxylipins , Plant Epidermis/growth & development , Plant Epidermis/metabolism , Plant Growth Regulators/pharmacology , Plant Leaves/growth & development , Plant Leaves/metabolism , Plants, Genetically Modified , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Stress, Mechanical
18.
Plant Physiol ; 130(4): 2019-26, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12481085

ABSTRACT

In higher plants, fat-storing seeds utilize storage lipids as a source of energy during germination. To enter the beta-oxidation pathway, fatty acids need to be activated to acyl-coenzyme As (CoAs) by the enzyme acyl-CoA synthetase (ACS; EC 6.2.1.3). Here, we report the characterization of an Arabidopsis cDNA clone encoding for a glyoxysomal acyl-CoA synthetase designated AtLACS6. The cDNA sequence is 2,106 bp long and it encodes a polypeptide of 701 amino acids with a calculated molecular mass of 76,617 D. Analysis of the amino-terminal sequence indicates that acyl-CoA synthetase is synthesized as a larger precursor containing a cleavable amino-terminal presequence so that the mature polypeptide size is 663 amino acids. The presequence shows high similarity to the typical PTS2 (peroxisomal targeting signal 2). The AtLACS6 also shows high amino acid identity to prokaryotic and eukaryotic fatty acyl-CoA synthetases. Immunocytochemical and cell fractionation analyses indicated that the AtLACS6 is localized on glyoxysomal membranes. AtLACS6 was overexpressed in insect cells and purified to near homogeneity. The purified enzyme is particularly active on long-chain fatty acids (C16:0). Results from immunoblot analysis revealed that the expression of both AtLACS6 and beta-oxidation enzymes coincide with fatty acid degradation. These data suggested that AtLACS6 might play a regulatory role both in fatty acid import into glyoxysomes by making a complex with other factors, e.g. PMP70, and in fatty acid beta-oxidation activating the fatty acids.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/genetics , Coenzyme A Ligases/genetics , Glyoxysomes/enzymology , Intracellular Membranes/enzymology , Amino Acid Sequence , Arabidopsis/enzymology , Arabidopsis/growth & development , Arabidopsis Proteins/isolation & purification , Arabidopsis Proteins/metabolism , Cloning, Molecular , Coenzyme A Ligases/isolation & purification , Coenzyme A Ligases/metabolism , DNA, Complementary/chemistry , DNA, Complementary/genetics , Gene Expression Regulation, Developmental , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Plant , Germination/genetics , Glyoxysomes/ultrastructure , Immunoblotting , Intracellular Membranes/ultrastructure , Microscopy, Immunoelectron , Molecular Sequence Data , Seeds/enzymology , Seeds/genetics , Seeds/growth & development , Sequence Analysis, DNA
19.
Plant Cell Physiol ; 43(10): 1086-95, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12407187

ABSTRACT

A novel vesicle, referred to as a precursor-accumulating (PAC) vesicle, mediates the transport of storage protein precursors to protein storage vacuoles in maturing pumpkin seeds. PV72, a type I integral membrane protein with three repeats of epidermal growth factor, was found on the membrane of the PAC vesicles. PV72 had an ability to bind to pro2S albumin, a storage protein precursor, in a Ca(2+)-dependent manner, via the C-terminal region of pro2S albumin, which was found to function as a vacuolar targeting signal. This implies that PV72 is a vacuolar sorting receptor of the storage protein. PV72 was specifically and transiently accumulated at the middle stage of seed maturation in association with the synthesis of storage proteins. Subcellular fractionation showed that PV72 was also accumulated in the microsomal fraction. A fusion protein consisting of GFP and the transmembrane domain and the cytosolic tail of PV72 was localized in Golgi complex. PV72 in the isolated PAC vesicles had a complex type of oligosaccharide, indicating that PV72 passed though the Golgi complex. These results suggest that PV72 is recycled between PAC vesicles and Golgi complex/post-Golgi compartments. PV72 appears to be responsible for recruiting pro2S albumin molecules from the Golgi complex to the PAC vesicles.


Subject(s)
Receptors, Cytoplasmic and Nuclear/metabolism , Seeds/physiology , Vacuoles/physiology , Arabidopsis/cytology , Arabidopsis/genetics , Arabidopsis/metabolism , Biological Transport/physiology , Calcium-Binding Proteins/metabolism , Clathrin-Coated Vesicles/physiology , Golgi Apparatus/physiology , Green Fluorescent Proteins , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Microscopy, Electron , Plant Proteins/genetics , Plant Proteins/metabolism , Receptors, Cytoplasmic and Nuclear/genetics , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Signal Transduction/physiology , Transfection , Vacuoles/ultrastructure
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