Impact of Induction Therapy on Delayed Graft Function Following Kidney Transplantation in Mated Kidneys.

BACKGROUND: Delayed graft function (DGF) is defined as the need for dialysis within 1 week of transplantation and occurs in 20%-50% of deceased-donor kidney transplant recipients. Although recovery from DGF often occurs within a few days, many cases may take weeks to months before the transplant function begins. The delay in function increases the complexity of recipient care, makes the diagnosis of acute rejection more difficult, prolongs length of stay, and increases hospital costs. Although several authors have proposed nomograms to predict DGF, there is no identifiable strategy to ameliorate it, except for the possible use of a specific type of induction therapy called Thymoglobulin. METHODS: In this retrospective analysis we included 407 subjects, of which 76 were mated (left and right kidney transplanted at Montefiore from the same donor). We used conditional logistic regression analysis while adjusting for the mated kidneys. We adjusted for age, gender, and race a priori, as well as cold ischemia time. RESULTS: There was a 36% decrease in odds of DGF when Thymoglobulin was used as induction when compared with basiliximab in mated kidneys 0.64 (0.10-4.05) (odds ratio [OR] with 95% confidence interval [CI]). CONCLUSIONS: Thymoglobulin did have a protective effect in these data when analyzed in mated kidneys, however, we need a larger amount of data to concretely conclude this effect.

A pathogenesis-based transcript signature in donor-specific antibody-positive kidney transplant patients with normal biopsies.

Affymetrix Human Gene 1.0-ST arrays were used to assess the gene expression profiles of kidney transplant patients who presented with donor-specific antibodies (DSAs) but showed normal biopsy histopathology and did not develop antibody-mediated rejection (AMR). Biopsy and whole-blood profiles for these DSA-positive, AMR-negative (DSA +/AMR-) patients were compared to both DSA-positive, AMR-positive (DSA +/AMR +) patients as well as DSA-negative (DSA -) controls. While individual gene expression changes across sample groups were relatively subtle, gene-set enrichment analysis using previously identified pathogenesis-based transcripts (PBTs) identified a clear molecular signature involving increased rejection-associated transcripts in AMR - patients. Results from this study have been published in Kidney International (Hayde et al., 2014 [1]) and the associated data have been deposited in the GEO archive and are accessible via the following link: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE50084.

Carbohydrate intake modulates the effect of the ABCA1-R230C variant on HDL cholesterol concentrations in premenopausal women.

The R230C variant of the ATP-binding cassette transporter A1 (ABCA1) gene has been consistently associated with decreased HDL-cholesterol (HDL-C) concentrations in several studies in the Mexican mestizo population. However, information on how diet composition modifies the effect of the ABCA1-R230C variant on HDL-C concentrations is very scarce. The aim of the present study was to analyze whether the effect of ABCA1-R230C on HDL-C concentrations is modulated by dietary factors in a nationwide population sample of 3591 adults from the National Health and Nutrition Survey conducted by the State's Employees' Social Security and Social Services Institute. All participants answered a validated questionnaire to assess health status and weekly food consumption. Fasting blood samples were drawn for biochemical analysis and DNA extraction, and the ABCA1-R230C variant was genotyped using TaqMan assays. Statistical analyses consisted of simple linear and multiple regression modeling adjusting for age, BMI, smoking, and alcohol consumption. The overall C risk allele frequency was 9.3% and the variant was significantly associated with low HDL-C concentrations in both sexes. A significant negative correlation between carbohydrate consumption and HDL-C concentrations was observed in women bearing the R230C variant (P = 0.021) and a significant gene-diet interaction was found only in premenopausal women (P = 0.037). In conclusion, the effect of the ABCA1-R230C gene variant on HDL-C concentrations is modulated by carbohydrate intake in premenopausal women. This finding may help design optimized dietary interventions according to sex and ABCA1-R230C genotype.

Attitudes of Jamaicans in Kingston and St.Andrew towards homosexuality [abstract]

OBJECTIVE: Jamaica has long been considered to be a homophobic society. The aim of this survey was to examine the attitudes of the Jamaican populance towards homosexuality, thus shedding light on the important issue of homosexuality, disclosure of one's sexual preference and the relationship to the HIV/AIDS epidemic. METHODS: There have been several subjective reports on the issue of homosexuality in Jamaica. Many of these reports have suggested that Jamaica is not truly a homophobic society. No objective study on attitudes towards homosexuality in Jamaica has been done to date; this is an attempt in that regard. A survey was conducted in the Kingston and St.Andrew region. A questionnaire consisting of 17 knowledge and attitude items with six items to determine demographic data was prepared. This was administered to every third person in 6 designated areas, ensuring that equal numbers of males and females aged 16 years and over were polled. RESULTS: The analysis indicates that the majority of those surveyed thought that the homosexuality lifestyle was unaccepatable. Of significance, views varied depending on the level of educational attainment: 100 percent of persons with primary level education found homosexuality unacceptable in contrast with 76 percent of those with teritiary level education with a similar opinion (p=0.028). There was a generally negative attitude towards befriending an acknowledged homosexual or having such persons in positions of authority. Many participants agreed that homosexual partners should have access to social services, including insurance plans, with 37 percent in outright agreement and 21 percent remaining neutral. There was a dramatic decrease in the number of persons who believed that HIV was solely a homosexual disease (13 percent) in contrast with the results of a 1989 survey conducted by the Ministry of Health (81 percent). CONCLUSION: Despite changing global opinions of the homosexual lifestyles, several biases still remain within the Jamaican society. These conditions force homosexuals to remain ensconced within the cloak of the more acceptable heterosexual lifestyle, which may have significant social and health implications. (AU)