Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1065-1066: 8-13, 2017 Oct 15.
Article in English | MEDLINE | ID: mdl-28938132

ABSTRACT

Agricultural pesticides used with the criminal intent to intoxicate domestic and wild animals are a serious concern in Veterinary Medicine. In order to identify the pesticide carbofuran and its metabolite 3- hydroxycarbofuran in animals suspected of exogenous intoxication a high pressure liquid chromatography with diode array detector (HPLC-DAD) method was developed and validated in stomach contents, liver, vitreous humor and blood. The method was evaluated using biological samples from seven different animal species. The following parameters of analytical validation were evaluated: linearity, precision, accuracy, selectivity, recovery and matrix effect. The method was linear at the range of 6.25-100µg/mL and the correlation coefficient (r2) values were >0.9811 for all matrices. The precision and accuracy of the method was determined by coefficient of variation (CV) and the relative standard deviation error (RSE), and both were less than 15%. Recovery ranged from 74.29 to 100.1% for carbofuran and from 64.72 to 100.61% for 3-hydroxycarbofuran. There were no significant interfering peaks or matrix effects. This method was suitable for detecting 25 positive cases for carbofuran amongst a total of 64 animal samples suspected of poisoning brought to the Toxicology Diagnostic Laboratory, School of Veterinary Medicine and Animal Sciences, University of Sao Paulo.


Subject(s)
Carbofuran/analogs & derivatives , Carbofuran/analysis , Chromatography, High Pressure Liquid/methods , Forensic Medicine/methods , Poisoning/diagnosis , Poisoning/veterinary , Veterinary Medicine/methods , Animals , Carbofuran/blood , Carbofuran/chemistry , Cats , Dogs , Gastrointestinal Contents/chemistry , Limit of Detection , Linear Models , Liver/chemistry , Reproducibility of Results , Vitreous Body/chemistry
SELECTION OF CITATIONS
SEARCH DETAIL
...