[The research programs and achievements of Guangxi Provincial Medical College during 1934-1945].

In 1934-1945, Guangxi Provincial Medical College continued to actively conducting scientific research and achieved fruitful results The research areas included medicine and pharmacy, with at least 43 research projects implemented and at least 130 research outputs. These achievements were made in a variety of ways, such as papers, monographs, compilations, anthologies, pharmaceutical products, diagnostic techniques, and instruments for therapy. Some of the achievements were awarded, and some others were widely applied clinically, contributing to the prevention and treatment of war injuries and the protection of people's health.

Impact of astrocyte and lymphocyte interactions on the blood-brain barrier in multiple sclerosis.

OBJECTIVE: This study was designed to investigate the impact of astrocyte and lymphocyte (LC) interactions in the blood-brain barrier (BBB) on the pathogenesis of multiple sclerosis (MS). METHODS: Primary rat brain microvascular endothelial cells (rBMECs) and astrocytes isolated from Sprague-Dawley rats were used to establish in vitro BBB models. Transendothelial electrical resistance (TEER) and permeability were compared between rBMEC monocultures and rBMEC/astrocyte co-cultures to evaluate the validity of each as a BBB cell model. rBMEC/LC co-cultures and rBMEC/astrocyte/LC tri-cultures were established to evaluate inflammatory responses in MS by measuring the gene expression levels of nerve growth factor (NGF), matrix metalloproteinase 2 (MMP-2), matrix metalloproteinase 9 (MMP-9), interleukin 17 (IL-17), interferon γ (IFN-γ), and forkhead box P3 (Foxp3). RESULTS: The rBMEC/astrocyte co-cultures exhibited higher TEER values and lower lymphocyte permeabilities than those of rBMEC monocultures. Compared to the rBMEC mono-cultures, the rBMEC/astrocyte/LC tri-cultures showed significantly decreased NGF, IL-17, and IFN-γ and increased MMP-2 and Foxp3 expression. Furthermore, the tri-cultures exhibited decreased NGF, IL-17, and IFN-γ expression compared to the rBMEC/astrocyte co-cultures, and increased MMP-2 expression compared to that shown by the rBMEC/LC co-cultures. MMP-9 expression did not vary significantly between the four established BBB cell models. CONCLUSION: These results suggest that the synergistic effect between astrocytes and LCs may increase the expression of MMP-2 and decrease that of IL-17 and IFN-γ at the BBB. Furthermore, the use of rBMEC/astrocytes/LC tri-cultures enabled us to test the synergistic effect between astrocytes and LCs and their roles in MS pathogenesis.

Meta-analysis of correlation between the CYP1A2 -3860 G > A polymorphism and lung cancer risk.

The aim of this meta-analysis was to assess the association between a polymorphism (-3860 G > A) in the cytochrome P450 1A2 (CYP1A2) gene and lung cancer susceptibility. Relevant studies were retrieved from the PubMed and EMBase databases, and additionally evaluated for conformance with the inclusion criteria. The odds ratios (ORs) and their 95% confidence intervals (95%CIs) in all selected studies were used to assess the relationship between the CYP1A2 -3860 G > A polymorphism and lung cancer risk. The data was pooled using Stata v.11. Six studies, comprising 1168 lung cancer patients and 1598 controls, were included in this meta-analysis. We found no correlation between the CYP1A2 -3860 G > A polymorphism and lung cancer risk in any of the models (AA vs GG: OR = 4.79, 95%CI = 0.03-702.67; GA vs GG: OR = 1.33, 95%CI = 0.74-2.39; dominant model: OR = 1.41, 95%CI = 0.69-2.90; recessive model: OR = 4.07, 95%CI = 0.04-368.35). Moreover, we observed no statistically significant association between CYP1A2 -3860 G > A and lung cancer susceptibility when stratified by the ethnicity of the sample populations, sample size, and study quality, except in a low-quality study. Our findings indicated that the -3860 G > A polymorphism in CYP1A2 might not be a risk factor for lung cancer.