ABSTRACT
The current study set out to elucidate the mechanism of miR-26b in OC cell proliferation and EMT via suppression of ERα. Initial findings illustrated that miR-26b was poorly expressed in OC tissues and cells. On the other hand, over-expression of miR-26b exerted a diminishing effect on SKOV3 cell proliferation, migration, invasion and EMT, whereas silencing of miR-26b conferred an enhancing effect on CAOV3 cell proliferation, migration, invasion and EMT. Subsequently, with help from the TargetScan database, a dual-luciferase reporter gene assay was carried out to verify the targeting relation between miR-26b and ERα, which revealed that miR-26b could negatively modulate ERα. Furthermore, the in vivo experimentation illustrated that over-expression of miR-26b led to down-regulation of ERα and suppression OC tumor growth and EMT. Meanwhile, silencing of ERα inhibited OC cell proliferation, migration, invasion and EMT. In conclusion, our findings indicated that miR-26b inhibited OC cell proliferation and EMT via negative-modulation of ERα. This investigation may offer potential strategy for OC treatment. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03222-2.
ABSTRACT
OBJECTIVE: To explore the difference in clinical efficacy and safety of transvaginal and transumbilical single port laparoscopy for endometrial cancer. METHODS: We retrospectively included 100 endometrial cancer patients who were admitted to the Fuzhou Second Hospital for surgical treatment from September 2020 to September 2021 and divided them into two groups according to different surgical treatment options. Patients in Group A (48 cases) were treated with transvaginal natural endoscopic surgery (TNES), and those in Group B (52 cases) were with transumbilical single port laparoscopic surgery (TSPLS). The operation time, intraoperative blood loss, time to postoperative exhaust, length of hospital stay, pelvic lymph node dissection, and incision infection rate of two groups were compared. The white blood cell count (WBC), hemoglobin (Hb), hematocrit (Hct) of the two groups of patients before and after the surgery were compared between the two groups, as well as th VAS score of 24 hours after the operation, rate of complications during hospitalization, satisfaction with surgery and quality of life 3 months after surgery. RESULTS: Compared with Group B, the operation time and intraoperative blood loss of Group A patients were markedly increased. The time to postoperative exhaust, length of hospital stay, incision infection rate, VAS score at postoperative 24 h, and complication rate of Group A were significantly lower than that of Group B. In addition, Group A had higher performance on the number of pelvic lymph node dissections, surgical satisfaction and quality of life 3 months after surgery. CONCLUSION: Transvaginal natural cavity endoscopy had better surgical results with faster postoperative recovery and higher safety compared with TSPLS, making it valuable in clinical application and worthy of further popularization.
