Interannual characteristics and driving mechanism of CO2 fluxes during the growing season in an alpine wetland ecosystem at the southern foot of the Qilian Mountains.

The carbon process of the alpine ecosystem is complex and sensitive in the face of continuous global warming. However, the long-term dynamics of carbon budget and its driving mechanism of alpine ecosystem remain unclear. Using the eddy covariance (EC) technique-a fast and direct method of measuring carbon dioxide (CO2) fluxes, we analyzed the dynamics of CO2 fluxes and their driving mechanism in an alpine wetland in the northeastern Qinghai-Tibet Plateau (QTP) during the growing season (May-September) from 2004-2016. The results show that the monthly gross primary productivity (GPP) and ecosystem respiration (Re) showed a unimodal pattern, and the monthly net ecosystem CO2 exchange (NEE) showed a V-shaped trend. With the alpine wetland ecosystem being a carbon sink during the growing season, that is, a reservoir that absorbs more atmospheric carbon than it releases, the annual NEE, GPP, and Re reached -67.5 ± 10.2, 473.4 ± 19.1, and 405.9 ± 8.9 gCm-2, respectively. At the monthly scale, the classification and regression tree (CART) analysis revealed air temperature (Ta) to be the main determinant of variations in the monthly NEE and GPP. Soil temperature (Ts) largely determined the changes in the monthly Re. The linear regression analysis confirmed that thermal conditions (Ta, Ts) were crucial determinants of the dynamics of monthly CO2 fluxes during the growing season. At the interannual scale, the variations of CO2 fluxes were affected mainly by precipitation and thermal conditions. The annual GPP and Re were positively correlated with Ta and Ts, and were negatively correlated with precipitation. However, hydrothermal conditions (Ta, Ts, and precipitation) had no significant effect on annual NEE. Our results indicated that climate warming would be beneficial to the improvement of GPP and Re in the alpine wetland, while the increase of precipitation can weaken this effect.

Study on the Heterogeneity of T-DM1 and the Analysis of the Unconjugated Linker Structure under a Stable Conjugation Process.

Trastuzumab emtansine (T-DM1) is a target-specific anticancer antibody-drug conjugate (ADC). In the present study, critical quality attributes for different manufactured products, such as the drug to antibody ratio (DAR), conjugation site, and site conjugation ratio, are similar, which is contrary to the traditional view that conjugation at lysine sites is randomly assigned. To investigate this result, a series samples with different DARs were prepared. Site conjugation ratios of the 27 different conjugation sites (corresponding to 54 potential sites) were analyzed. We found that the correlation coefficients of the 26 site conjugation ratio and the DAR were R 2 > 0.9, and the remaining one was R 2 > 0.7. By comparing three batches of samples with a DAR value of ∼3.3 in a stability study, we found that degradation rates of conjugation sites of the samples incubated at 40 °C were basically the same. These data show that the conjugation ratio and the conjugation stability of each site may remain consistent if the process parameters are stable. LC/MS/MS was used to study the unconjugated linker of the crosslink byproducts produced by a two-step method. We determined four forms of unconjugated linkers: (N-maleimidomethyl) cyclohexane-1-carboxylate (MCC) unconjugated to DM1, hydrolyzed MCC unconjugated to DM1, lys-MCC-lys, and lys-MCC-cys. We believe that the current study can provide an effective guide for the processing of ADCs, control of product quality, and reduction of side reaction products.