Revealing the mechanisms of Arisaema cum Bile on allergic asthma with systematic pharmacology approach-experimental validation.

Arisaema cum Bile (Dan Nanxing in Chinese, DNX) have been employed to treat allergic asthma. However, the active components and its mechanisms remain unknown. Therefore, the systematic pharmacology approach-experimental validation was performed in this study. Each 5, 6, and 10 compounds of DNX were obtained by HPLC analysis, TCMSP, and literature report, respectively. A total of 379 targets on all these compounds were acquired from Swiss Target Prediction, and 1973 targets on allergic asthma were predicated. The KEGG enrichment analysis was performed. Furthermore, a rat model of allergic asthma was established and DNX (450 mg/kg, p.o.) was given for 2 weeks. DNX treatment prevented OVA-induced pathological changes in lung cell of irregular arrange and necrotic bronchial epithelial. It also decreased inflammatory cytokines IL-4, IL-5, and IL-13 of serum and BALF, and increased IL-12 and IFN-γ. The main MAPK signaling pathway predicted by KEGG enrichment was verified, as indicated by the decreased protein expression of JNK (p < 0.05 & p < 0.01), ERK (p < 0.05), and p38 MAPK (p < 0.01) in lung tissue. These findings indicated that DNX attenuated OVA-induced allergic asthma mainly by decreasing the MAPK signaling pathway.

What motivates farmers to adopt low-carbon agricultural technologies? Empirical evidence from thousands of rice farmers in Hubei province, central China.

Low-carbon agriculture is essential for protecting the global climate and sustainable agricultural economics. Since China is a predominantly agricultural country, the adoption of low-carbon agricultural technologies by local farmers is crucial. The past literature on low-carbon technologies has highlighted the influence of demographic, economic, and environmental factors, while the psychological factors have been underexplored. A questionnaire-based approach was used to assess the psychological process underlying the adoption of low-carbon agricultural technologies by 1,114 Chinese rice farmers in this paper, and structural equation modeling (SEM) was empirically employed to test our theoretical model. The results indicated that farmers' low-carbon production attitude and behavioral efficiency perception directly and positively affected the adoption of low-carbon agricultural technologies and indirectly affected it via low-carbon production intention. Besides, production implementation cost and socio-environmental factor could moderate the direct effects of low-carbon production attitude, behavioral efficiency perception, and low-carbon production intention on farmers' adoption of low-carbon agricultural technologies. In this respect, socio-environmental factor yielded more significant moderating effects. Additionally, this research provides policy implications for promoting low-carbon agricultural technologies in developing countries and regions.

Taxifolin improves inflammatory injury of human bronchial epithelial cells by inhibiting matrix metalloproteinase (MMP) 10 via Wnt/ß-catenin pathway.

Taxifolin (TXL), also known as dihydroquercetin, is one of the most important flavonoids prevalent across the plant kingdom. Increasing evidence has demonstrated its critical role in respiratory diseases. The present study aims to reveal the detailed mechanism in TNF-α-stimulated BEAS-2B cells by which TXL might exert effects on the development of asthma. Cell viability detection of BEAS-2B treated with TXL before and after TNF-α induction employed MMT. The expressions of inflammatory cytokines, MUC5AC and ICAM-1 were determined by quantitative reverse transcription PCR (RT-qPCR), enzyme-linked immunosorbent assay (ELISA) and Western blot after TXL was exposed to an in vitro asthma model. Then, light transmittance and apoptosis were then measured employing fluorescein transmittance, TUNEL and Western blot. After overexpressing MMP10, the abovementioned assays were performed again. Finally, the association between Wnt/ß-catenin pathway and MMP10 was confirmed by detecting the proteins in this pathway. TXL increases the cell viability of TNF-induced BEAS-2B cells. TXL suppressed the inflammation, mucus formation, and apoptosis in TNF-α-induced BEAS-2B cells. Furthermore, after the prediction of binding sites between TXL and MMP10, it was found that overexpression of MMP10 reversed the effects of TXL on suppressing the progression of TNF-α-induced BEAS-2B cells. Finally, TXL blocked Wnt/ß-catenin pathway by inhibiting MMP10 expression.TXL can be a promising drug for the treatment of asthma due to its inhibition of MMP10 expression by blocking Wnt/ß-catenin pathway. Future experimental in vivo studies of asthma on this commonly used bioactive flavonoid could open new avenues for the therapies of asthma.

Influence of incentive mechanism and fit degree on user's environmental behavior-Taking Alipay "Ant Forest" in China as an example.

How to use game elements to motivate users and influence their behavior has become a new research trend, which is vital for enhancing the willingness of potential platform users to participate in environmental protection. This paper aims to analyze the influence of incentive mechanism and fit degree on user's environmental behavior based on the stimulus-organism-response theory and self-determination theory. The questionnaire data of 500 users was collected and the impact of incentives on user's environmental behavior was analyzed by structural equation modeling. The results show that economic, value, and social incentives have a significant impact on user's environmental behavior. Besides, the value and social incentives of "Ant Forest" game platform positively influence user fit (conscious participation, enthusiasm, and platform interaction), but the impact of economic incentive on platform interaction is not statistically significant. From the perspective of user fit, "Ant Forest" game platform can positively promote users to adopt environmental behavior, because it explores users' needs from their perspective to give full play to the role of game incentives on users' environmental behavior. Additionally, this research provides the practical implications for managers exploring the effects of co-creation processes in developing countries and regions.

Botanical, Traditional Use, Phytochemical, and Toxicological of Arisaematis rhizoma.

MATERIALS AND METHODS: This review is a collection of all possible studies on AR, published in scientific journals, papers, and books. Using the papers related to Arisaematis, such as ScienceDirect, Wiley Online Library, Springer Link, Web of Science, CNKI, and WanFang Database. In this paper, the traditional uses, botany, phytochemistry, pharmacology, and toxicology of AR were reviewed. Finally, the existing problems and research directions of the research on AR are discussed. RESULTS: Ninety-eight chemical constituents were isolated from AR. AR has a wide range of pharmacological effects, such as the effects on the central nervous system and cardiovascular system. It also has anti-tumor, sedative, analgesic, anticonvulsant, anti-inflammatory, expectorant, antiarrhythmic, anticoagulant, and other effects. It is also considered an effective drug for in vitro and in vivo validation. CONCLUSIONS: AR is an excellent traditional medicinal plant in China. Pharmacological studies support the traditional use of AR and may verify the folk use of AR in the treatment of different diseases. The anti-tumor effect of AR has been widely concerned by scholars at home and abroad. It has become a hot spot in recent years and has made great contributions to the survival and development of human beings. Although it has a high value of comprehensive utilization, its development and utilization are far from enough. Therefore, the comprehensive development of AR is worthy of further analysis.

Research on Frequency Doubling Effect of Thermoacoustic Speaker Based on Graphene Film.

In this work, the frequency doubling effect of thermoacoustic speakers is studied, and a method is analyzed to suppress the frequency doubling effect. Three cases were analyzed by superimposing the DC bias on the AC excitation: (1) DC is less than AC; (2) DC is equal to AC; (3) DC is greater than AC. We found that the frequency doubling effect can be well suppressed by superimposing a larger DC excitation on the AC excitation. The laser scribing technology was used to prepare graphene film in only one step, and the screen printing technology was used to prepare conductive electrodes. The microphone and B&K system was used to record the sound pressure level and study the suppression of frequency doubling effect. Finally, the sound pressure levels with the three different kinds of excitations were measured. The measured results show that they have a good agreement with the theoretical results. The suppression effect will be better when DC amplitude is greater than AC amplitude. Therefore, this work has certain reference significance for the further study and application of thermoacoustic speakers.

Botanical and Traditional Uses and Phytochemical, Pharmacological, Pharmacokinetic, and Toxicological Characteristics of Ziziphi Spinosae Semen: A Review.

Ziziphi Spinosae Semen (ZS, the seeds of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou) is used as a traditional Chinese medicine referred to as Suan zao ren (). This paper aims to provide a systematic review of its traditional uses and its botanical, phytochemical, pharmacological, pharmacokinetic, and toxicological characteristics. The future development and research prospects for ZS have also been discussed in detail. To date, over 150 compounds have been identified in this plant, including terpenoids, alkaloids, flavonoids, fatty acids, volatile oils, polysaccharides, and others. Both extracts and purified compounds have excellent biological activities, especially sedative and hypnotic effects. Other effects include ameliorating effect of learning and memory, anti-inflammation, antioxidation, blood pressure and lipid lowering, antiaging, and antitumor effects. Thus, this traditional Chinese medicine can be used to treat many diseases such as insomnia, forgetfulness, headaches, and dizziness. Although many of the traditional uses of ZS are well established, the relationship between structure and function still needs to be further studied. In order to better pave the way for research and the establishment of quality control standards for ZS, it will be very important to elucidate its pharmacological mechanisms of action and explore new clinical effects.

Serum S100A12 and 30-day mortality after acute intracerebral hemorrhage.

BACKGROUND: S100A12 is implicated in inflammatory reactions. The purpose of this study was to determine the association between serum S100A12 concentrations and 30-day mortality in patients with acute intracerebral hemorrhage (ICH). METHODS: We prospectively included 182 healthy controls and 182 ICH patients within 24h after stroke onset. Serum samples were collected for the measurement of S100A12 concentrations. Multivariable analysis was used to evaluate the relationship between serum S100A12 concentrations and mortality of ICH patients within 30days. RESULTS: Serum S100A12 concentrations were significantly increased compared to control subjects. S100A12 concentrations were positively correlated with National Institutes of Health Stroke Scale (NIHSS) score, ICH volume, blood glucose concentrations, blood white blood cell count and plasma C-reactive protein concentrations. 36 (19.8%) patients were deceased within 30days after stroke onset. Non-survivors had significantly higher concentrations of serum S100A12 than survivors. Additionally, Serum S100A12 concentrations significantly discriminated patients at risk of 30-day mortality and its predictive value was equivalent to those of NIHSS score and hematoma volume. Moreover, higher serum S100A12 concentrations showed a significantly higher risk for 30-day mortality and overall survival. CONCLUSIONS: Higher S100A12 concentrations are positively associated with inflammation, hemorrhagic severity and short-term mortality among ICH patients, indicating S100A12 may represent a biomarker for predicting poor outcome after hemorrhagic stroke.

Prognostic value of serum thioredoxin concentrations after intracerebral hemorrhage.

BACKGROUND: Thioredoxin (TRX) is a potent anti-oxidant and its circulating concentration is increased in some critical illnesses. We measured the serum TRX concentrations and further investigated the relationship between serum TRX concentrations and hemorrhagic severity and outcome after intracerebral hemorrhage (ICH). METHODS: A total of 218 ICH patients and 218 healthy controls were enrolled in this prospective, observatory study. Serum TRX concentrations were determined using an enzyme-linked immunosorbent assay. Hemorrhagic severity was assessed with National Institutes of Health Stroke Scale (NIHSS) score and hematoma volume. Clinical endpoint was 6-month mortality. RESULTS: Compared with the controls, the patients had elevation of serum TRX concentrations (24.4±11.5 ng/ml vs. 8.4±3.3 ng/ml, P<0.001). Serum TRX concentrations were highly related to NIHSS score (r=0.532, P<0.001) and hematoma volume (r=0.486, P<0.001). Serum TRX concentrations higher than 29.6 ng/ml was an independent predictor of 6-month mortality (odds ratio, 3.978; 95% confidence interval, 1.486-10.649) and 6-month overall survival (hazard ratio, 3.511; 95% confidence interval, 1.827-6.746). TRX concentrations improved the predictive value of NIHSS score and hematoma volume for 6-month mortality. CONCLUSIONS: Increased serum TRX concentration, related closely to hemorrhagic severity and long-term mortality, has the potential to be a novel prognostic predictive biomarker after ICH.