Characterization of the active components and bioaccessibility of phenolics in differently colored foxtail millets.

Differently colored foxtail millet (Setaria italica) cultivars were compared regarding their amylose, B-complex vitamin, vitamin E, and phenolic compositions, as well as the bioaccessibility of their phenolics in simulated in vitro digestion. Dark-colored foxtail millets contained more thiamine, pyridoxine, and tocopherols, but less riboflavin, than light-colored ones. Phenolics were more abundant in dark-colored cultivars. Insoluble bound fractions accounted for 75%-83% of the total phenolics, with ferulic acid detected as the most plentiful compound. The major bioaccessible phenolic was free ferulic acid, with 100%-120% bioaccessibility, depending on cultivar, followed by p-coumaric acid and isoferulic acid (50%-80%). These relatively high bioaccessibilities were likely due to the release of soluble conjugated or insoluble bound phenolics during digestion. However, the contents of other free phenolics were largely decreased following in vitro digestion, resulting in low bioaccessibility, which also means that the release from the conjugated and bound fractions was poor.

New perspective: Symbiotic pattern and assembly mechanism of Cantharellus cibarius-associated bacteria.

Cantharellus cibarius, an ectomycorrhizal fungus belonging to the Basidiomycetes, has significant medicinal and edible value, economic importance, and ecological benefits. However, C. cibarius remains incapable of artificial cultivation, which is thought to be due to the presence of bacteria. Therefore, much research has focused on the relationship between C. cibarius and bacteria, but rare bacteria are frequently overlooked, and symbiotic pattern and assembly mechanism of the bacterial community associated with C. cibarius remain unknown. In this study, the assembly mechanism and driving factors of both abundant and rare bacterial communities of C. cibarius were revealed by the null model. The symbiotic pattern of the bacterial community was examined using a co-occurrence network. Metabolic functions and phenotypes of the abundant and rare bacteria were compared using METAGENassist2, and the impacts of abiotic variables on the diversity of abundant and rare bacteria were examined using partial least squares path modeling. In the fruiting body and mycosphere of C. cibarius, there was a higher proportion of specialist bacteria compared with generalist bacteria. Dispersal limitation dominated the assembly of abundant and rare bacterial communities in the fruiting body and mycosphere. However, pH, 1-octen-3-ol, and total phosphorus of the fruiting body were the main driving factors of bacterial community assembly in the fruiting body, while available nitrogen and total phosphorus of the soil affected the assembly process of the bacterial community in the mycosphere. Furthermore, bacterial co-occurrence patterns in the mycosphere may be more complex compared with those in the fruiting body. Unlike the specific potential functions of abundant bacteria, rare bacteria may provide supplementary or unique metabolic pathways (such as sulfite oxidizer and sulfur reducer) to enhance the ecological function of C. cibarius. Notably, while volatile organic compounds can reduce mycosphere bacterial diversity, they can increase fruiting body bacterial diversity. Findings from this study further, our understanding of C. cibarius-associated microbial ecology.

Investigation on the Microbial Diversity of Fresh-Cut Lettuce during Processing and Storage Using High Throughput Sequencing and Their Relationship with Quality.

Microbial community distribution in vegetables can affect their quality. This study analyzed the distribution of the microbial community at various stages during processing and storage with the microbial diversity analysis, and evaluated the correlation between the dominant bacteria and sensory quality of lettuce using correspondence analysis with multiple regression analysis. Results showed that the process of washing, cutting, then disinfection and dewatering could change the community distribution and dominant bacteria in lettuce, and maintain better texture, morphology, aroma, color qualities of lettuce. The total number of colonies and relative abundance of Xanthomonas in fresh-cut lettuce decreased, while Afipia and Ralstonia increased during processing and pre-storage (storage for 6 h, 12 h and 1 d). After storage for 3 d, the total number of colonies in lettuce increased (more than 5 log CFU/g), especially the relative abundance of Pseudomonas, which led to the obvious deterioration of the sensory quality of lettuce. Throughout the process, the number of Bacillus cereus, Staphylococcus aureus, and E. coli was less than 100 CFU/g and 3 MPN/g. The number of typical pathogenic bacteria, Salmonella, Listeria monocytogenes and E. coli O157:H7, was below the detection limit. Overall, the prevention and control of psychrotrophic Pseudomonas in lettuce was still necessary. These results will provide useful information for the fresh-cut lettuce industry.

Tissue distribution of phthalates in celery under different cultivation patterns and associated dietary exposure.

To investigate tissue distribution, spatial difference, temperature variation, and potential health risks of PAEs in vegetables, celery was used as a model plant. Celery samples were collected from open fields and greenhouses from two provinces in China over four seasons. Celery tissues were analyzed for 16 PAE compounds by gas chromatography-tandem mass spectrometry. The total content of PAEs was 89.0-1130.3 µg kg-1 dry weight (dw) in stems and 155.0-2730.8 µg kg-1 dw in leaves. Concentrations of PAEs in celeries showed notable spatial differences (P < 0.05), and the levels in samples from open fields were lower than those in samples from plastic greenhouses. In celeries from greenhouses, higher PAE concentrations were observed for plants grown at high temperatures than in plants grown at low temperatures. Discrepancies in tissue distribution indicated different uptake pathways of PAE contaminants. Risk assessments to humans found that both carcinogenic risks and non-carcinogenic risks of PAEs via celery consumption were at an acceptable level. Further research should consider other exposure pathways of PAEs and pay special attention to reducing PAE contents in vegetables.

Investigating Heritage Language Processing: Meaning Composition in Chinese Classifier-Noun Phrasal Contexts.

The aim of the present study was to investigate how Chinese-Malay bilingual speakers with Chinese as heritage language process semantic congruency effects in Chinese and how their brain activities compare to those of monolingual Chinese speakers using electroencephalography (EEG) recordings. To this end, semantic congruencies were manipulated in Chinese classifier-noun phrases, resulting in four conditions: (i) a strongly constraining/high-cloze, plausible (SP) condition, (ii) a weakly constraining/low-cloze, plausible (WP) condition, (iii) a strongly constraining/implausible (SI) condition, and (iv) a weakly constraining/implausible (WI) condition. The analysis of EEG data focused on two event-related potential components, i.e., the N400, which is known for its sensitivity to semantic fit of a target word to its context, and a post-N400 late positive complex (LPC), which is linked to semantic integration after prediction violations and retrospective, evaluative processes. We found similar N400/LPC effects in response to the manipulations of semantic congruency in the mono- and bilingual groups, with a gradient N400 pattern (WI/SI > WP > SP), a larger frontal LPC in response to WP compared to SP, SI, and WI, as well as larger centro-parietal LPCs in response to WP compared to SI and WI, and a larger centro-parietal LPC for SP compared to SI. These results suggest that, in terms of event-related potential (ERP) data, Chinese-Malay early bilingual speakers predict and integrate upcoming semantic information in Chinese classifier-noun phrase to the same extent as monolingual Chinese speakers. However, the global field power (GFP) data showed significant differences between SP and WP in the N400 and LPC time windows in bilinguals, whereas no such effects were observed in monolinguals. This finding was interpreted as showing that bilinguals differ from their monolingual peers in terms of global field power intensity of the brain by processing plausible classifier-noun pairs with different congruency effects.

Magnetically controlled colorimetric aptasensor for chlorpyrifos based on copper-based metal-organic framework nanoparticles with peroxidase mimetic property.

The fabrication of a magnetically controlled colorimetric aptasensor for chlorpyrifos is reported. The aptasensor was fabricated by the attachment of the colorimetric labels onto the magnetic carrier due to the hybridization reaction between the complementary DNA and aptamer. Chlorpyrifos detection was realized by monitoring the color changes of the TMB/H2O2 solution before and after incubation of the aptasensor with chlorpyrifos via exposure to external magnetic force. The color change was monitored at 650 nm by UV-Vis spectrophotometer. Under the optimal conditions, this magnetically controlled Cu-MOF-based aptasensor showed a detection limit of 4.4 ng/mL with a linear range of 0-1250 ng/mL. The colorimetric aptasensor displayed high selectivity for chlorpyrifos toward other interfering pesticides. The aptasensor was successfully applied for the spiked test of chlorpyrifos in fruits and vegetable samples with good recovery, which were in agreement with data obtained by GC-MS analysis. This magnetically controlled Cu-MOF-based sensing strategy not only leads to development of efficient and facile phase separation, but also expands the MOF's target scope from H2O2 or glucose to pesticides. Graphical abstract.