ABSTRACT
OBJECTIVE: To investigate the contents of glycyrrhizic acid in hejian decoction (mixed the traditional Chinese herbs together, then boiling them with water) and the fenjian decoction (boiling the single traditional Chinese herb with water separately, then mixed the abstracts) of Sanaotang (composed of Ephedra sinica, Prunus armeniaca and Glycyrrhiza uralensis) and to compare with their anti-bacterial activities in vitro. METHOD: A HPLC method was established with a Agilent Zorbax SB-C18 column (4. 6 mm x 250 mm, 5 microm), a mobile phase of acetonitrile-0.2% acetic acid solution (35:65), a flow rate of 1 mL x min(-1) and a detection wavelength of 254 nmn in order to determine the contents of glycyrrhizic acid minimal bacterial inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) antagonized the common bacteria in different decoctions were rieasured in vitro by employing dilution method. RESULT: The average content of glycyrrhizic acid of the hejian decoction was higher than that of the fenjian decoction. The hejian decoction could display the inhibitory bactericidal activity to Aeruginosus bacillus, but the fenjian decoction could not. And to Staphylococcus aureus, the inhibitory bactericidal activity the hejian decoction was slightly stronger than that of the fenjian decoction. CONCLUSION: Comparing with that of the fenjian decoction, the content of glycyrrhizic acid of the hejian decoction was higher and the anti-bacterial activities was stronger.
Subject(s)
Antifungal Agents/pharmacology , Chromatography, High Pressure Liquid/methods , Drugs, Chinese Herbal/chemistry , Glycyrrhizic Acid/analysis , Glycyrrhizic Acid/pharmacology , Anti-Bacterial Agents/analysis , Anti-Bacterial Agents/pharmacology , Antifungal Agents/analysis , Aspergillus niger/drug effects , Aspergillus niger/growth & development , Bacteria/drug effects , Bacteria/growth & development , Drug Combinations , Drugs, Chinese Herbal/isolation & purification , Ephedra sinica/chemistry , Glycyrrhiza uralensis/chemistry , Hot Temperature , Microbial Sensitivity Tests , Plants, Medicinal/chemistry , Prunus/chemistry , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/growth & development , Staphylococcus aureus/drug effects , Staphylococcus aureus/growth & developmentABSTRACT
OBJECTIVE: To investigate the factors influencing on the ephedrine contents in different compositions of Maxingshigan decoction. METHOD: Adopt mixed uniform design to dismantle recipes, employ the stepping regression analysis to deal with experimental statistics, use the partial correlational analysis to analyze the correlation coefficients and the results were validated. RESULT: The regressive equation was of significance, that is Y = 4.36719347 + 7.752707437X1 + 1.2557197041X3 (r = 0.85564, P = 0.0189). The main influencing factors on ephedrine content in Maxingshigan decoction were gypsum and amygdalin. The influent factor of amygdalin on ephedrine content had great significance, and gypsum had significance. CONCLUSION: The collective effects of amygdalin and gypsum affect the content of ephedrine in Maxing Shigan decoction which the content-effect relationship was in direct correlation.
Subject(s)
Amygdalin/chemistry , Calcium Sulfate/chemistry , Drugs, Chinese Herbal/chemistry , Ephedra sinica/chemistry , Ephedrine/analysis , Amygdalin/isolation & purification , Drug Combinations , Drugs, Chinese Herbal/isolation & purification , Glycyrrhiza uralensis/chemistry , Herb-Drug Interactions , Materia Medica/chemistry , Plants, Medicinal/chemistry , Prunus/chemistryABSTRACT
OBJECTIVE: To determine the contents of amygdalin in traditional and granular decoctions of Maxingshigan decoction. METHODS: The determination was carried out by HPLC with an ODS column and a mobile phase of water-methanol (23:77) at 215 nm. RESULTS: The content of amygdalin in traditional decoction of Maxingshigan Decoction was 17.74 mg/g and 24.80 mg/g in granular one. CONCLUSION: The content of glycyrrhizic acid in traditional decoction of Maxingshigan decoction is obviously higher than that in granular one.
Subject(s)
Amygdalin/analysis , Chromatography, High Pressure Liquid/methods , Drugs, Chinese Herbal/chemistry , Plants, Medicinal/chemistry , Calcium Sulfate/chemistry , Drug Combinations , Drugs, Chinese Herbal/isolation & purification , Glycyrrhiza uralensis/chemistry , Glycyrrhizic Acid/analysis , Reproducibility of Results , Seeds/chemistryABSTRACT
OBJECTIVE: To compare the contents of baicalin in the raw medical material and the different prepared medical materials of Scutellaria baicalensis. METHOD: The contents of baicalin were determined by HPLC. Chromatographic conditions included Hypersil C18 column and the mobile phase consisting of a mixture of methanol-0.04% phosphoric acid (46:54). Baicalin was detected at 280 nm. The standard curve was linear in the range of 0.0280-4.6700 micrograms with correlation coefficient 1.0000. The average recovery of baicalin was 101.22% with RSD = 2.54% (n = 5). RESULT: The contents of baicalin were 6.80% in raw material, 6.00% and 6.73% in the different prepared medical materials of S. baicalensis respectively. CONCLUSION: The two different kinds of preparing methods can be used to the raw material of S. baicalensis.
