Chemical composition, source characteristics, and hygroscopic properties of organic-enriched aerosols in the high Arctic during summer.

Arctic regions are extremely sensitive to global warming. Aerosols are one of the most important short-lived climate-forcing agents affecting the Arctic climate. The present study examines the summertime chemical characteristics and potential sources of various organic and inorganic aerosols at a Norwegian Arctic site, Ny-Ålesund (79°N). The results show that organic matter (OM) accounts for 60 % of the total PM10 mass, followed by sulfate (SO42-). Water-soluble organic carbon (WSOC) contributes 62 % of OC. Photochemical processes involving diverse anthropogenic and biogenic precursor compounds are identified as the major sources of WSOC, while water-insoluble organic carbon (WIOC) aerosols are predominantly linked to primary marine emissions. Despite being a remote pristine site, the aerosols show a sign of chemical aging, evidenced by a significant chloride depletion, which was about 82 % on average during the study period. Nitrogen-containing aerosols are likely stemming from migratory seabird colonies and local dust sources around the sampling site. While biogenic, crustal, and sea salt-derived SO42- account for 37%, 8%, and 5% respectively, the remaining 50% is attributed to anthropogenic SO42-. Through chemical tracers, Pearson correlation coefficient matrix, and Hierarchical Cluster Analysis (HCA), the present study identifies soil biota (terrestrial biogenic) and marine emissions, along with their photochemical oxidation processes, as potential sources of Arctic aerosols during summer, while biomass burning and combustion-related sources have a minor contribution. The chemical closure of hygroscopicity highlights that while organics predominantly control aerosol hygroscopicity in the Arctic summer, specific inorganic components like (NH4)2SO4 can significantly increase it on certain days, affecting aerosol-cloud interactions and climate processes over the Arctic during summer. The present study highlights the high abundance of organics and their vital role in the Arctic climate during summer when natural aerosols are conquered.

Atmospheric aerosol chemistry and source apportionment of PM10 using stable carbon isotopes and PMF modelling during fireworks over Hyderabad, southern India.

This study examined the influence of fireworks on atmospheric aerosols over the Southern Indian city of Hyderabad during festival of Diwali using mass closure, stable carbon isotopes and the EPA-PMF model. Identification of chemical species in day and night time aerosol samples for 2019 and 2020 Diwali weeks showed increased concentrations of NH4+, NO3-, SO42-, K+, organic carbon (OC), Ba, Pb and Li, which were considered as tracers for fireworks. PM10 source apportionment was done using inorganic (trace elements, major ions) and carbonaceous (organic and elemental carbon; OC & EC) constituents, along with stable isotopic compositions of TC and EC. K+/Na+ ∼1 and K+nss/OC > 0.5 indicated contribution from fireworks. High NO3-, NH4+, Na+, Cl- and SO42- suggested the presence of deliquescent salts NaCl, NH4NO3 and (NH4)2SO4. TAE/TCE >1 suggested H+ exclusion, indicating possible presence of H2SO4 and NH4HSO4 in the aerosols. Ba, Pb, Sb, Sr and Fe increased by 305 (87), 12 (11), 12 (3), 3 (2) and 3 (4) times on Diwali nights, compared to pre-Diwali of 2019 (2020), and are considered as metallic tracers of fireworks. δ13CTC and δ13CEC in aerosols closely resembled that of diesel and C3 plant burning emissions, with meagre contribution from firecrackers during Diwali period. The δ13CEC was relatively depleted than δ13CTC and δ13COC. For both years, δ13COC-EC (δ13COC - δ13CEC) were positive, suggesting photochemical aging of aerosols during long-range transport, while for pre-Diwali 2019 and post-Diwali 2020, δ13COC-EC were negative with high OC/EC ratio, implying secondary organic aerosols formation. High toluene during Diwali week contributed to fresh SOA formation, which reacted with precursor 12C, leading to 13C depletions. Eight-factored EPA-PMF source apportionment indicated highest contribution from residue/waste burning, followed by marine/dust soil and fireworks, while least was contributed from solid fuel/coal combustion.

Assessing the Efficacy of Anterior Repositioning Splints in the Management of Temporomandibular Disc Displacement: A Systematic Review and Meta-Analysis.

Disc displacement (DD) occurs when the cushioning disc of the temporomandibular joint (TMJ), situated between the jawbone and the skull, is out of position. The condition can be of two kinds: disc displacement with reduction (ddwr) and disc displacement without reduction (ddwor). The present systematic review was undertaken to assess the efficacy of anterior repositioning splints (ARS) for ddwr and ddwor. Three online databases were searched for relevant studies using MeSH keywords and Boolean operators. Initial searches of the databases extracted 552 records. Twelve studies equally representing ARS with ddwr and ddwor were selected. No clear difference could be assessed regarding ARS usage in both conditions. The outcome assessed was the improvement in TMJ symptoms. The results suggested that both ddwr and ddwor were effective in improving temporomandibular joint (TMJ) symptoms in patients who received ARS therapy. Efficacy levels ranged from 71-83% and 50-95% for ARS in ddwr and ddwor, respectively; odds ratio (OR) values ranging from 0.30 and 0.36 were obtained for ARS in both therapies, respectively, indicating similar performance levels. Both ddwr and ddwor respond well to the use of ARS; however, more clinical trials are needed to ascertain and validate the role of ARS as a treatment modality in this regard.

Water-soluble organic aerosols over South Asia - Seasonal changes and source characteristics.

Water-soluble organic carbon (WSOC) has been identified as a key component in atmospheric aerosols due to its ability to act as cloud condensation nuclei (CCN) owing to their highly hygroscopic nature. This paper discusses about the spatio-temporal variability in WSOC mass concentration, sources (primary and secondary contributions), the role of long-range air-mass transport in modulating their abundance, at distinct sectors over South Asia. We found from our observations that, photochemical ageing of primary organic aerosols that are derived from biomass emissions, significantly contribute to the total WSOC budget over South Asia. The wide range of water-soluble compounds released by biomass burning can contribute directly to the WSOC fraction or undergo further atmospheric processing, such as oxidation or ageing, leading to the formation of additional WSOC. WSOC/OC (organic carbon) ratio and the correlation between the WSOC and secondary organic carbon (SOC) are used for assessing the contribution from secondary sources. The three different ratios are used to delineate different source processes; OC/EC (elemental carbon) for source identification, WSOC/OC for long-range atmospheric transport (ageing) and WSOC/SOC to understand the primary and secondary contribution of WSOC. The present investigation revealed that, the primary OC that have undergone significant chemical processing as a result of long-range transport have a substantial influence on WSOC formation over South Asia, especially in Indo Gangetic Plain outflow regions such as southern peninsular and adjacent marine regions. Overall, oxidation and ageing of primary organic aerosols emitted from biomass burning was found to serve as an important source of WSOC over South Asia.

Radiographical Evaluation of Morphological Alterations of Mandibular Third Molars: A Cone Beam Computed Tomography (CBCT) Study.

Introduction  Morphological changes or variations in the lower third molar can be of concern during the endodontic, orthodontic, or prosthetic intervention. The goal of the present study was to evaluate the morphological alterations in the roots and root canal of a mandibular third molar in Bhopal, Central India, on cone beam computed tomography (CBCT) images. Methodology CBCT scans of 277 mandibular molars, of both genders, between the ages of 18 and 60 years were assessed for the presence of root numbers, the configuration of the canal based on Vertucci's categorization, and a C-shaped canal. Scan results were analyzed for differences in canal configuration between the roots and topographical distribution. A chi-square test was applied to find any significant differences between the teeth at p 0.05. Results Scans analyzed for variations in the third molar had a mean age of 38.64 + 5.71 years. The majority (95.3%) of the molars had two roots, 1.5% had three roots, and 0.4% had five roots. The mesial side of double-rooted teeth predominantly had Type II canal configuration (67.0%), while it was Type I (79.2%) in the distal aspect of the root. C-shaped canals were detected in 21 teeth, and no significant topographical difference was noted in the CBCT images. Conclusion The majority of the current population showed two roots with the same number of canals in the studied tooth. CBCT can be used as a diagnostic aid in identifying the canal numbers and their configuration so as to render appropriate intervention and minimize subsequent failure.

Exploratory analysis of demographic data, tobacco habits, and oral health-related quality of life among complete denture patients.

Aim: The present study assessed the impact of demographics and tobacco habits on oral health related quality of life (OHRQoL) among complete denture patients. Setting and Design: Prospective cohort study design. Materials and Methods: Two hundred and eighty four edentulous patients, aged above 30 years, were chosen as the sample for the study after taking informed consent. Information regarding demographic data and smoking status was obtained from each participant. OHRQoL was evaluated using the Oral Health Impact Profile Edentulousness (OHIP EDENT) quantifying various domains, namely functional limitation, physical pain, psychological discomfort, physical disability, psychological disability, social disability, and handicap. Statistical Analysis Used: Data were analyzed using the Statistical Package for the Social Sciences version 23.0 (IBM; Chicago, Illinois, USA). Mann-Whitney U test was applied to find significant differences in OHRQoL between gender, age, and smoking status. P < 0.05 was considered to be statistically significant. Results: It was seen that complete denture wearers above 65 years had higher OHIP EDENT scores as compared to younger counterparts suggesting the compromised quality of life in the functional domain which was statistically significant. Psychological discomfort was greater in females while physical disability seemed higher in males. Smoking was found to be a factor associated with decreased OHRQoL. Conclusion: Older age groups, female gender, and smoking were factors associated with decreased OHRQoL among complete denture patients.

Effect of physical and psychological status on oral health quality of life of geriatric patients undergoing complete denture treatment.

Aim: The present study was conducted to evaluate differences in Oral Health-Related Quality of Life (OHRQoL) in denture wearers based on psychological classification and patient satisfaction. Settings and Design: A prospective study. Materials and Methods: 284 patients, aged 30 years and above who fulfilled the eligibility criteria were recruited. Participants answered the OHIP - EDENT questionnaire at the time of denture insertion and 6 months later. Psychological categorization was based on MM House classification. Patient satisfaction was graded from totally satisfied to not very satisfied. Statistical Analysis Used: SPSS 23 version was used for analyzing descriptive and inferential statistics. ANOVA was used to find significant differences for OHRQoL based on psychological classification and patient satisfaction. Before and after intervention analysis was assessed using student 't' test. Results: Philosophical and exacting patients had better adaptation to dentures than the hysterical and indifferent class of denture wearers. Totally satisfied and very satisfied patients with dentures had lesser mean scores as against the other categories which was significant in all domains suggesting better. Overall, OHIP - EDENT score decreased from 20.253 ± 12.466 to 17.168 ± 14.143, which were significant at P =0.043, thus showing an improvement after a 6 month follow up. Conclusions: Psychological attitude of denture wearers must be considered by the prosthetic specialist for effective adaptation and acceptance by the edentulous patient.

Molecular composition and light-absorbing properties of organic aerosols from west-coast of tropical India.

Tropical coastal regions may provide a unique feature to study the photooxidation of various organic aerosols and their climatic effects because of high humid atmosphere and intense solar radiation. However, knowledge about organic molecular composition and its light absorption properties remains concealed, particularly over tropical Indian regions. The present study is an investigation on water-soluble dicarboxylic acids, ω-oxoacids, pyruvic acid, α-dicarbonyls, brown carbon (BrC), and other chemical species in PM1.1 collected at a coastal urban location (Kochi) on the west coast of tropical India under distinct air masses. Molecular distribution of dicarboxylic acids was characterized by the predominance of oxalic acid (C2) in all the air masses followed by adipic (C6) or terephthalic (tPh) and phthalic (Ph) acids. On average, total diacids-C accounted for 5.03 ± 1.01 % of TC. Total diacid concentration showed strong linear relationships with organic (OC), elemental carbon (EC), and non-sea-salt potassium (nss-K+). Except for the northwest (NW) air mass period, the concentration of C2 diacid and its ratios (C2/total diacids, C2/ωC2, C2/Gly) showed a strong linear relationship with nss-SO42-. By combining all these results together with Pearson correlation analysis, the present study demonstrates that organic aerosols over the study region were predominantly produced by aqueous-phase oxidation of precursor compounds derived from biomass burning and combustion-related emissions. The mass absorption coefficient of BrC (babs-BrC-365nm) was strongly correlated with nss-K+, implying that biomass burning emissions are major sources of BrC. The absorption angstrom exponent (AÅE) values of water (methanol) extracts ranged from 3.20 to 3.83 (3.05-4.55) during the entire sampling period, indicating the substantial contribution of BrC chromophores to light absorption over the region. On average, BrC absorbs 10.6 ± 6.4 % and 22.4 ± 5.75 % of solar radiation compared to BC in water and methanol extracts, respectively, suggesting that BrC is a significant aerosol climate forcing agent over the west coast of tropical India.

Seasonal and vertical characteristics of particulate and elemental concentrations along diverse street canyons in South India.

The impact of street geometries on vertical dispersion of PMs (PM2.5 and PM10) in (1) non-street canyon (NSC), (2) street canyon (SC), and (3) street canyon with viaduct (SCV) was studied during four seasons. The chemical composition of the species was analysed for source apportionment. The mass concentration of PMs in canyons was in the order of SCV > SC > NSC, implicating the canyon effect. Independent of height, most of the PM concentrations in SC and SCV violated the National Ambient Air Quality Standards (NAAQS) and exceeded the World Health Organization (WHO) guidelines in all three street geometries. The vertical concentration trend of PMs was significant during winter and summer seasons in NSC and SC. The vertical trend of both PMs was significant during summer and monsoon seasons in SCV. The seasonal change in PMs' vertical trend was influenced by atmospheric stability, wind velocities associated with street morphology, and emission sources. The ratio of PM2.5/PM10 indicated the dominance of PM10 in all three locations. Among the estimated species, Fe (crustal and vehicle) and Na (sea salt and crustal) were abundant in PM2.5 and PM10, respectively. Estimation of enrichment factor (EF) revealed that most of the emission sources were anthropogenic in PM2.5 and natural in PM10. Principal component analysis (PCA) showed crustal/soil dust, vehicular emission, and sea salt to the common source profile for PMs. Specific contribution of smoking activity contributed to Be and Tl in PM2.5, which may be considered a site-specific source.

A Clinical Evaluation of Difference in Shades among Maxillary Central Incisor, Canine, and First Molar in a Young Age-group Using Digital Spectrophotometer: An In Vivo Study.

AIM: The aim of this study was to evaluate and compare the most common shades of maxillary central incisor, canine and first molar and to confirm the shade difference between maxillary central incisor and canine in a young population of 18-25 years. MATERIALS AND METHODS: The shade of the maxillary central incisor, canine, and first molar of 100 study participants in a young population between 18 and 25 years were measured by digital spectrophotometer (VITA Easyshade). The shade of each tooth was assessed thrice with a digital spectrophotometer at the center of the tooth. Statistical analysis was performed; Chi-squared test was applied to assess the difference in shades. RESULTS: For the age-group of 18-25 years, the most common shade of maxillary central incisor is A1 and for canine and first molar the most common shade is B3. A highly statistically significant difference (p < 0.001) was observed between teeth, suggesting a definitive shade difference between teeth. CONCLUSION: A definitive shade difference exists between the maxillary central incisor and the canine, with the canine being darker in shade than the central incisor. This result can be implied clinically while restoring maxillary anterior teeth to yield a better esthetic outcome. CLINICAL SIGNIFICANCE: This study reveals that there is a definitive shade difference between the Anterior teeth which should be considered while smile designing to replicate the natural appearance in a patient. Using a digital spectrometer makes the process of shade selection objective thereby eliminating any subjective variations.

Geochemical characteristics of trace elements in size-resolved coastal urban aerosols associated with distinct air masses over tropical peninsular India: Size distributions and source apportionment.

Trace elements in atmospheric particulate matter play a significant role in air quality, health and biogeochemical cycles. The present study reports on geochemical characteristics of size-resolved trace elements in PM10 aerosols collected under different air masses over a coastal urban location in peninsular India. A contrast in elemental distribution was observed for the particle size above 7.0 µm and below 1.1 µm under the influence of northeasterly air masses as characterized by Al > Fe > Zn and Fe > Al > Zn, respectively. The concentrations of the crustal elements (Al, Fe, Ti, P, Ba, Co) were high and illustrated by a unimodal size distribution with a peak in coarse mode (>2.0 µm) during northwesterly air masses. On the other hand, combustion-derived metals (Cu, Zn, Cd, Sb, and Pb) were maximized under northeasterly air masses, characterized by unimodal size distribution with a peak in fine mode (<2.0 µm). The enrichment factor (EF) analysis reveals the contribution of anthropogenic emissions to Cd, Sb, Pb, Zn, Cu, Cr, Ni, As, and Sn metals, particularly to the high enrichment of trace metals in fine mode. These results suggest that crustal emissions are major sources of trace metals in coarse mode aerosols; whereas combustion derived anthropogenic emissions contribute to the fine mode aerosols. The positive matrix factorization (PMF) analysis revealed that crustal sources (52-90%) were most abundant for particles >7.0 µm, whereas combustion related emissions such as vehicular and traffic sources are predominant for particles <1.1 µm. The present study demonstrates that trace metals in coastal urban aerosols are affected by changes in emission sources/strengths and regional transport of air masses originated from the northeasterly and northwesterly parts of the tropical Indian subcontinent.

Continental outflow of anthropogenic aerosols over Arabian Sea and Indian Ocean during wintertime: ICARB-2018 campaign.

Chemical characterisation of atmospheric aerosols over Arabian Sea (AS) and Indian Ocean (IO) have been carried out during the winter period (January to February 2018) as part of the Integrated Campaign for Aerosols, gases and Radiation Budget (ICARB-2018). Mass concentrations of organic carbon (OC), elemental carbon (EC), water soluble and insoluble OC (WSOC, WIOC), primary and secondary OC (POC, SOC), water-soluble inorganic ions and trace metals have been estimated with a view to identify and quantify the major anthropogenic pollutants affecting the oceanic environments. Aerosol mass loading was found to exhibit strong spatial heterogeneity (varying from 13 to 84 µg m-3), significantly modulated by the origin of air-mass trajectories. Chemical analysis of aerosols revealed the presence of an intense pollution plume over south-eastern coastal Arabian Sea, near to south-west Indian peninsula (extending from ~ 12°N to 0° at 75°E) with a strong latitudinal gradient (~3 µg m-3/deg. from north to south) dominated by anthropogenic species contributing as high as 73% (38% nss-SO42-, 24.2% carbonaceous aerosols (21% Organic Matter, 3.2% EC) and 10% NH4+). Anthropogenic signature over oceanic environment was also evident from the dominance and high enrichment of elements like Zn, Cu, Mn and Pb in trace metals. Long-range transport of air-masses originating from Indo Gangetic Plains and its outflow regions in Bay of Bengal, has been seen over Arabian Sea during winter, that imparted such strong anthropogenic signatures over this oceanic environment. Comparison with previous cruise studies conducted nearly two decades ago shows a more than two-fold increase in the concentration of nss-SO42-, over the continental outflow region in Arabian Sea.

Seasonal changes in carbonaceous aerosols over a tropical coastal location in response to meteorological processes.

Near-surface atmospheric aerosols (PM10) collected from a tropical coastal location in south-west peninsular Indian region for a duration of 6 years (2012-18) (N = 461) were analysed for carbonaceous aerosol components, the less studied aerosol species. Organic carbon (OC), its water soluble-insoluble (WSOC and WIOC) components, primary-secondary (POC and SOC) fractions and elemental carbon (EC) were examined for understanding the annual, seasonal, day-night variations in abundance pattern along with associated physical and meteorological processes. Total carbonaceous aerosols accounting for 36% of the collected aerosol mass with 31.5% organic matter (OM) and 4.5% EC respectively, exhibited consistent seasonal pattern throughout the study period with high concentration during winter followed by post-monsoon, pre-monsoon and monsoon. Delineation of marine and continental components of carbonaceous species based on their relative dominance during different air-mass periods, shows that while marine aerosols were a combination of natural sources comprising of volatile, semi-volatile species and secondary organics (from marine VOC precursors); the continental aerosols were composed of anthropogenic combustion sources (fossil fuel, biomass emissions etc). Based on the measurements of OC and EC during 2005-09 and 2012-18, their long term trends (for more than a decade) were investigated. Although OC showed an increasing tendency, EC exhibited a decrease with the total carbonaceous aerosols exhibiting a gradual decreasing trend over the years, indicating that they do not strictly reverberate the reported increasing trend observed over north-central parts of India. This can be presumed to be due to the reduced anthropogenic inputs over the location owing to the control measures and policies. The strong convective activity and large scale monsoon phenomena also helps in the effective dispersion of pollutants. Making use of comprehensive measurement of carbonaceous aerosols and the previous measurements of other aerosol components, an improved chemical composition model is presented.

Comparison of Healthy and Dandruff Scalp Microbiome Reveals the Role of Commensals in Scalp Health.

Several scalp microbiome studies from different populations have revealed the association of dandruff with bacterial and fungal dysbiosis. However, the functional role of scalp microbiota in scalp disorders and health remains scarcely explored. Here, we examined the bacterial and fungal diversity of the scalp microbiome and their potential functional role in the healthy and dandruff scalp of 140 Indian women. Propionibacterium acnes and Staphylococcus epidermidis emerged as the core bacterial species, where the former was associated with a healthy scalp and the latter with dandruff scalp. Along with the commonly occurring Malassezia species (M. restricta and M. globosa) on the scalp, a strikingly high association of dandruff with yet uncharacterized Malassezia species was observed in the core mycobiome. Functional analysis showed that the fungal microbiome was enriched in pathways majorly implicated in cell-host adhesion in the dandruff scalp, while the bacterial microbiome showed a conspicuous enrichment of pathways related to the synthesis and metabolism of amino acids, biotin, and other B-vitamins, which are reported as essential nutrients for hair growth. A systematic measurement of scalp clinical and physiological parameters was also carried out, which showed significant correlations with the microbiome and their associated functional pathways. The results point toward a new potential role of bacterial commensals in maintaining the scalp nutrient homoeostasis and highlights an important and yet unknown role of the scalp microbiome, similar to the gut microbiome. This study, therefore, provides new perspectives on the better understanding of the pathophysiology of dandruff.

Chemical Constituents of Carbonaceous and Nitrogen Aerosols over Thumba Region, Trivandrum, India.

Aerosol filter samples collected at a tropical coastal site Thumba over Indian region were analysed for water-soluble ions, total carbon and nitrogen, organic carbon (OC), elemental carbon (EC), and water-soluble organic carbon/nitrogen and their sources for different seasons of the year. For the entire study period, the order of abundance of ions showed the dominance of secondary ions, such as SO42-, NO3-, and NH4+. On average, Mg2+ (56%), K+ (11%), SO42- (8.8%), and Ca2+ (8.1%) contributions were from maritime influence. There was significant chloride depletion due to enhanced levels of inorganic acids, such as SO42- and NO3-. Total carbon contributed 21% of the aerosol total suspended particulate matter in which 85% is organic carbon. Primary combustion-generated carbonaceous aerosols contributed 41% of aerosol mass for the entire study period. High average ratios of OC/EC (5.5 ± 1.8) and WSOC/OC (0.38 ± 0.11) suggest that organic aerosols are predominantly comprised of secondary species. In our samples, major fraction (89 ± 9%) was found to be inorganic nitrate in total nitrogen (TN). Good correlations (R 2 ≥ 0.82) were observed between TN with NO3- plus NH4+, indicating that nitrate and ammonium ions account for a significant portion of TN. The temporal variations in the specific carbonaceous aerosols and air mass trajectories demonstrated that several pollutants and/or their precursor compounds are likely transported from north western India and the oceanic regions.

Organic and inorganic components of aerosols over the central Himalayas: winter and summer variations in stable carbon and nitrogen isotopic composition.

The aerosol samples were collected from a high elevation mountain site, Nainital, in India (1958 m asl) during September 2006 to June 2007 and were analyzed for water-soluble inorganic species, total carbon, nitrogen, and their isotopic composition (δ(13)C and δ(15)N, respectively). The chemical and isotopic composition of aerosols revealed significant anthropogenic influence over this remote free-troposphere site. The amount of total carbon and nitrogen and their isotopic composition suggest a considerable contribution of biomass burning to the aerosols during winter. On the other hand, fossil fuel combustion sources are found to be dominant during summer. The carbon aerosol in winter is characterized by greater isotope ratios (av. -24.0‰), mostly originated from biomass burning of C4 plants. On the contrary, the aerosols in summer showed smaller δ(13)C values (-26.0‰), indicating that they are originated from vascular plants (mostly of C3 plants). The secondary ions (i.e., SO4 (2-), NH4 (+), and NO3 (-)) were abundant due to the atmospheric reactions during long-range transport in both seasons. The water-soluble organic and inorganic compositions revealed that they are aged in winter but comparatively fresh in summer. This study validates that the pollutants generated from far distant sources could reach high altitudes over the Himalayan region under favorable meteorological conditions.

Transposition/Fusion: A Clinician's Dilemma and Challenge - 12 Months Follow-up.

Fusion or syndontia is a sequele of the union of two normal and separated tooth buds. Transposition refers to the interchange in the position of two permanent teeth within the same quadrant in the oral cavity. The simultaneous incidence of both these entities is a rare concurrence and warrants endodontic and surgical soft tissue correction. The following manuscript describes a case report of this rare combination and its multidisciplinary management for functional and esthetic correction.

A spectro photometric comparative evaluation of apical sealing ability of three different sealers; calcium hydroxide based, resin based and zinc oxide eugenol based sealers.

BACKGROUND: The aim of this study was to quantitatively analyze the amount of dye leakage with AH26, Sealapex and Tubliseal sealers in endodonticaly treated teeth. MATERIALS AND METHODS: A total of 36 extracted mandibular molar specimens were divided into three groups; Group I: Sealapex, Group II: Tubliseal, Group II: AH26 with 12 samples in each group. Standard access cavity and biomechanical preparation was done with step back flare technique. Obturations were done using respective sealers in the three different groups. Then, samples were subjected to spectro photometric analysis using a filter of 670 nm. Spectrophotometric analysis was performed to quantitatively analyze the amount of dye leakage with all three sealers. RESULTS: Tubliseal exhibited the least microleakage. In comparison to Sealapex, Tubliseal showed a significant difference. Compared to Sealapex, AH26 showed no significant difference. Comparisons made between Tubliseal and AH26 showed no significant difference. CONCLUSION: In the present study, Tubliseal sealer showed least microleage compared with Sealapex and AH26 sealer.

In vivo effect of whole grain flour of finger millet (Eleusine coracana) and kodo millet (Paspalum scrobiculatum) on rat dermal wound healing.

Influence of finger millet and kodo millet on rat dermal wound healing was assessed by making a 4 cm2 (2 x 2 cm) excision wound on the shaven back of rats under ether anesthesia. Finger millet or kodo millet flour (300 mg) as aqueous paste was applied topically once daily for 16 days. The granulation tissue formed on day 4, 8 and 12 was used to estimate some biochemical parameters like protein, DNA, collagen and lipid peroxides. There was significant increase in protein and collagen contents and decrease in lipid peroxides. Biophysical parameters like rate of contraction and number of days for epithelialization were also studied. Rate of contraction was 88-90% in kodo millet and finger millet treated rats in comparison to 75% in untreated rats. The number of days for complete closure of wounds was lower for finger millet (13 days) and kodo millet (14 days) treated rats in comparison to untreated (16 days) rats. The results implicate a possible therapeutical role for finger millet and kodo millet in accelerating the process of wound healing.

Inhibition of collagen glycation and crosslinking in vitro by methanolic extracts of Finger millet (Eleusine coracana) and Kodo millet (Paspalum scrobiculatum).

The present investigation was carried out to study the effects of methanolic extracts of Finger millet (Eleusine coracana) and Kodo millet (Paspalum scrobiculatum) on glycation and crosslinking of collagen. Tail tendons obtained from rats weighing 200-225 g were incubated with glucose (50 mM) and 3 mg of extracts of the above millets in methanol under physiological conditions of temperature and pH for 10 days. Early glycation was estimated by phenol-sulfuric acid method and the crosslinking was assessed by pepsin digestion, cyanogen bromide peptide map and viscosity measurements. Tendon collagen incubated with glucose (50 mM) showed 65% solubility on pepsin treatment; poor resolution of bands in the cyanogen bromide peptide map, and intrinsic viscosity of 0.84 dl/g. The collagen incubated with Finger millet and Kodo millet extracts inhibited glycation; 89% and 92% solubility in pepsin; good resolution of bands in the cyanogen bromide peptide map and intrinsic viscosity of 0.46 and 0.58 dl/g respectively. The study implicates the potential usefulness of the above millets in protection against glycation and crosslinking of collagen.