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1.
J Am Soc Nephrol ; 30(4): 678-691, 2019 04.
Article in English | MEDLINE | ID: mdl-30846560

ABSTRACT

BACKGROUND: Hypertension and cardiovascular disease are common in children undergoing dialysis. Studies suggest that hemodiafiltration (HDF) may reduce cardiovascular mortality in adults, but data for children are scarce. METHODS: The HDF, Heart and Height study is a nonrandomized observational study comparing outcomes on conventional hemodialysis (HD) versus postdilution online HDF in children. Primary outcome measures were annualized changes in carotid intima-media thickness (cIMT) SD score and height SD score. RESULTS: We enrolled 190 children from 28 centers; 78 on HD and 55 on HDF completed 1-year follow-up. The groups were comparable for age, dialysis vintage, access type, dialysis frequency, blood flow, and residual renal function. At 1 year, cIMT SD score increased significantly in children on HD but remained static in the HDF cohort. On propensity score analysis, HD was associated with a +0.47 higher annualized cIMT SD score compared with HDF. Height SD score increased in HDF but remained static in HD. Mean arterial pressure SD score increased with HD only. Factors associated with higher cIMT and mean arterial pressure SD-scores were HD group, higher ultrafiltration rate, and higher ß2-microglobulin. The HDF cohort had lower ß2-microglobulin, parathyroid hormone, and high-sensitivity C-reactive protein at 1 year; fewer headaches, dizziness, or cramps; and shorter postdialysis recovery time. CONCLUSIONS: HDF is associated with a lack of progression in vascular measures versus progression with HD, as well as an increase in height not seen in the HD cohort. Patient-related outcomes improved among children on HDF correlating with improved BP control and clearances. Confirmation through randomized trials is required.


Subject(s)
Body Height , Carotid Intima-Media Thickness , Hemodiafiltration , Kidney Failure, Chronic/blood , Kidney Failure, Chronic/therapy , Adolescent , Blood Pressure , C-Reactive Protein , Child , Child, Preschool , Dizziness/etiology , Female , Headache/etiology , Hemodiafiltration/adverse effects , Hemodiafiltration/methods , Hemoglobins/metabolism , Hospitalization , Humans , Hypertension/etiology , Kidney Failure, Chronic/complications , Male , Muscle Cramp/etiology , Parathyroid Hormone/blood , Patient Reported Outcome Measures , Phosphates/blood , Renal Dialysis/adverse effects , Young Adult , beta 2-Microglobulin/blood
2.
BMC Nephrol ; 19(1): 199, 2018 08 10.
Article in English | MEDLINE | ID: mdl-30097064

ABSTRACT

BACKGROUND: Cardiovascular disease is prevalent in children on dialysis and accounts for almost 30% of all deaths. Randomised trials in adults suggest that haemodiafiltration (HDF) with high convection volumes is associated with reduced cardiovascular mortality compared to high-flux haemodialysis (HD); however paediatric data are scarce. We designed the haemodiafiltration, heart and height (3H) study to test the hypothesis that children on HDF have an improved cardiovascular risk profile, growth and nutritional status and quality of life, compared to those on conventional HD. We performed a non-randomised parallel-arm intervention study within the International Paediatric Haemodialysis Network Registry comparing children on HDF and conventional HD to determine annualised change in cardiovascular end-points and growth. Here we present the 3H study design and baseline characteristics of the study population. METHODS: 190 children were screened and 177 (106 on HD and 71 on HDF) recruited from 28 centres in 10 countries. There was no difference in age, underlying diagnosis, comorbidities, previous dialysis therapy, dialysis vintage, residual renal function, type of vascular access or blood flow between HD and HDF groups. High flux dialysers were used in 63% of HD patients and ultra-pure water was available in 52%. HDF patients achieved a median convection volume of 13.3 L/m2; this was associated with the blood flow rate only ((p = 0.0004, r = 0.42) and independent of access type (p = 0.38). DISCUSSION: This is the largest study on dialysis outcomes in children that involves deep phenotyping across a wide range of cardiovascular, anthropometric, nutritional and health-related quality of life measures, to test the hypothesis that HDF leads to improved cardiovascular and growth outcomes compared to conventional HD. TRIAL REGISTRATION: ClinicalTrials.gov: NCT02063776 . The trial was prospectively registered on the 14 Feb 2014.


Subject(s)
Body Height/physiology , Cardiovascular Diseases/prevention & control , Child Development/physiology , Heart/physiology , Hemodiafiltration/trends , Kidney Failure, Chronic/therapy , Adolescent , Cardiovascular Diseases/diagnosis , Cardiovascular Diseases/psychology , Child , Child, Preschool , Female , Hemodiafiltration/methods , Hemodiafiltration/psychology , Humans , Kidney Failure, Chronic/diagnosis , Kidney Failure, Chronic/psychology , Male , Prospective Studies , Quality of Life/psychology , Renal Dialysis/methods , Renal Dialysis/psychology , Renal Dialysis/trends , Treatment Outcome , Young Adult
3.
Kidney Int ; 91(4): 937-947, 2017 04.
Article in English | MEDLINE | ID: mdl-28117080

ABSTRACT

Steroid Resistant Nephrotic Syndrome (SRNS) in children and young adults has differing etiologies with monogenic disease accounting for 2.9-30% in selected series. Using whole exome sequencing we sought to stratify a national population of children with SRNS into monogenic and non-monogenic forms, and further define those groups by detailed phenotypic analysis. Pediatric patients with SRNS were identified via a national United Kingdom Renal Registry. Whole exome sequencing was performed on 187 patients, of which 12% have a positive family history with a focus on the 53 genes currently known to be associated with nephrotic syndrome. Genetic findings were correlated with individual case disease characteristics. Disease causing variants were detected in 26.2% of patients. Most often this occurred in the three most common SRNS-associated genes: NPHS1, NPHS2, and WT1 but also in 14 other genes. The genotype did not always correlate with expected phenotype since mutations in OCRL, COL4A3, and DGKE associated with specific syndromes were detected in patients with isolated renal disease. Analysis by primary/presumed compared with secondary steroid resistance found 30.8% monogenic disease in primary compared with none in secondary SRNS permitting further mechanistic stratification. Genetic SRNS progressed faster to end stage renal failure, with no documented disease recurrence post-transplantation within this cohort. Primary steroid resistance in which no gene mutation was identified had a 47.8% risk of recurrence. In this unbiased pediatric population, whole exome sequencing allowed screening of all current candidate genes. Thus, deep phenotyping combined with whole exome sequencing is an effective tool for early identification of SRNS etiology, yielding an evidence-based algorithm for clinical management.


Subject(s)
Genomics/methods , Mutation , Nephrotic Syndrome/congenital , Precision Medicine , Adolescent , Age of Onset , Child , Child, Preschool , Cohort Studies , Disease Progression , Exome , Female , Genetic Association Studies , Genetic Predisposition to Disease , Heredity , High-Throughput Nucleotide Sequencing , Humans , Infant , Intracellular Signaling Peptides and Proteins/genetics , Kaplan-Meier Estimate , Kidney/pathology , Kidney Failure, Chronic/diagnosis , Kidney Failure, Chronic/genetics , Kidney Failure, Chronic/therapy , Male , Membrane Proteins/genetics , Nephrotic Syndrome/diagnosis , Nephrotic Syndrome/genetics , Nephrotic Syndrome/therapy , Pedigree , Phenotype , Predictive Value of Tests , Prognosis , Registries , Risk Factors , United Kingdom , WT1 Proteins/genetics , Young Adult
4.
Trials ; 15: 147, 2014 Apr 27.
Article in English | MEDLINE | ID: mdl-24767719

ABSTRACT

BACKGROUND: Relapses of childhood steroid-sensitive nephrotic syndrome (SSNS) are treated with a 4- to 8-week course of high-dose oral prednisolone, which may be associated with significant adverse effects. There is a clear association between upper respiratory tract infection (URTI) and relapse development. Previous studies in developing nations have suggested that introducing a 5- to 7-day course of daily prednisolone during an URTI may prevent a relapse developing and the need for a treatment course of high-dose prednisolone. The aim of PREDNOS 2 is to evaluate the effectiveness of a 6-day course of daily prednisolone therapy during an URTI in reducing the development of a subsequent relapse in a developed nation. METHODS/DESIGN: The subjects will be 300 children with relapsing SSNS (≥2 relapses in preceding year), who will be randomised to receive either a 6-day course of daily prednisolone or no change to their current therapy (with the use of placebo to double blind) each time they develop an URTI over 12 months. A strict definition for URTI will be used. Subjects will be reviewed at 3, 6, 9 and 12 months to capture data regarding relapse history, ongoing therapy and adverse effect profile, including behavioural problems and quality of life. A formal health economic analysis will also be performed. The primary end point of the study will be the incidence of URTI-related relapse (3 days of Albustix +++) following the first infection during the 12-month follow-up period. DNA and RNA samples will be collected to identify a potential genetic cause for the disease. Subjects will be recruited from over 100 UK centres with the assistance of the Medicines for Children Research Network.PREDNOS 2 is funded by the National Institute for Health Research Health Technology Assessment Programme (11/129/261). DISCUSSION: We propose that PREDNOS 2 will be a pivotal study that will inform the future standard of care for children with SSNS. If it is possible to reduce the disease relapse rate effectively and safely, this will reduce the morbidity and cost associated with drug treatment, notwithstanding hospital admission and parental absence from employment. TRIAL REGISTRATION: Current Controlled Trials (ISRCTN10900733).


Subject(s)
Glucocorticoids/administration & dosage , Nephrotic Syndrome/drug therapy , Prednisolone/administration & dosage , Research Design , Respiratory Tract Infections/drug therapy , Adolescent , Child , Child, Preschool , Clinical Protocols , Cost-Benefit Analysis , Double-Blind Method , Drug Administration Schedule , Drug Costs , Glucocorticoids/economics , Humans , Nephrotic Syndrome/diagnosis , Nephrotic Syndrome/economics , Prednisolone/economics , Recurrence , Respiratory Tract Infections/diagnosis , Respiratory Tract Infections/economics , Time Factors , Treatment Outcome , Young Adult
5.
Nephron Physiol ; 114(2): p19-24, 2010.
Article in English | MEDLINE | ID: mdl-20068363

ABSTRACT

AIM: To describe the clinical features and genetic basis of distal renal tubular acidosis (dRTA) in Filipino children. METHODS: Clinical description and gene analysis of affected members of 7 families. RESULTS: In all affected children, the disease was associated with mutations of the SLC4A1 gene that codes for the bicarbonate/chloride anion-exchanger 1 (AE1, band 3) protein situated in the red cell membrane and the alpha-intercalated (proton-secreting) cell of the renal collecting duct. In 2 families, affected children were homozygous for a substitution of aspartic acid for glycine in residue 701 of the AE1 protein (G701D); in the other 5 families, affected children were compound heterozygotes of this mutation with the AE1 mutation (Delta400-408) that causes Southeast Asian ovalocytosis (SAO). All affected children had morphological red cell changes that closely resembled SAO, including the children who were homozygous for G701D and did not have the SAO mutation. Homozygous G701D thus produces morphological red cell changes that are not readily distinguishable from SAO. The parents of all 7 families were originally domiciled in the islands of the Visayas group in the central part of the Philippine archipelago. CONCLUSION: Recessive renal tubular acidosis in Filipinos is usually caused by SLC4A1 mutations, commonly G701D.


Subject(s)
Acidosis, Renal Tubular/genetics , Anion Exchange Protein 1, Erythrocyte/genetics , Acidosis, Renal Tubular/diagnosis , Asian People/genetics , Child , Elliptocytosis, Hereditary/diagnosis , Elliptocytosis, Hereditary/genetics , Erythrocyte Membrane/genetics , Erythrocyte Membrane/physiology , Female , Genes, Recessive , Humans , Kidney Tubules, Collecting/physiology , Male , Mutation , Philippines/epidemiology
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