Non-TIR-NBS-LRR resistance gene analogs in apricot (Prunus armeniaca L.).

Genes encoding for proteins with nucleotide-binding site and leucine-rich repeat motifs (NBS-LRR) have been suggested to play a general role in plant defence mechanism. In Prunus species, many TIR (Toll / Interleukin-1 Receptor), and only very few non-TIR sequences were identified, which was explained either by the unequal distribution of TIR/non-TIR sequences in the Prunus genome or by the incapability of primers in the amplification of non-TIR RGAs. The objective of this work was to check whether a new semi-nested PCR strategy can be developed for the targeted isolation of non-TIR-NBS-LRR Resistance Gene Analog (RGA) sequences from apricot. Three primers (CUB-P-loop F, CUB-Kin2 F and CUB-HD R) were designed, from which CUB-Kin2 F and CUB-HD R were constructed to anneal selectively to the non-TIR sequences. A colony Polymerase Chain Reaction (PCR) indicated that out of the 96 clones tested 28 showed amplification using the newly developed primers, while no amplification occurred when using the formerly described primers. Half of the 28 positive clones were sequenced and they turned out to represent 11 different non-TIR RGA sequences. A phylogenetic analysis was carried out based on an alignment containing 293 Rosaceae and 21 non-Rosaceaa sequences. A significantly higher ratio (91%) of non-TIR sequences were arranged in multi-genera clades than that of (57%) the TIR groups confirming that non-TIR sequences might be of more ancient origin than TIR sequences.

Assessment and prevalence of depression in women 45-55 years of age visiting gynecological clinics in Poland: screening for depression among midlife gynecologic patients.

The aims of the Polish survey were to assess efficacy of screening for depression in gynecological practice and to estimate prevalence of depressive disorders in midlife women visiting gynecologists. The study included 2262 female outpatients aged 45-55, who were screened by 120 gynecologists throughout Poland. Patients completed the Beck's Depression Inventory (BDI) and were assessed by gynecologists to verify the presence of symptoms of a current Depressive Episode according to ICD-10 diagnostic criteria. Patients who obtained a score of 12 points or more on the BDI were referred for psychiatric evaluation, including the modified version of Mini International Neuropsychiatric Interview (MINI). The study showed that gynecologists in Poland are able to perform screenings for depression effectively in outpatient settings. Results also suggested that about 19% of women aged 45 to 55 years visiting gynecologists may suffer from depressive disorders.

Challenges encountered in measuring outcome for a rural psychiatric residential program.

Evaluation of outcome measures can provide policymakers with valuable information on the effectiveness of psychiatric rehabilitation. Two specific challenges in collecting outcome measures for psychiatric rehabilitation programs are heterogeneity of outcomes and difficulty with follow-up. These two challenges were illustrated in the process of evaluating Rose Hill Center, a rural residential psychiatric rehabilitation program. The original design was to conduct interviews with former residents and family members and verify healthcare utilization. The difficulty of locating people and their reluctance to participate conspired to lower the follow-up rates. The design was modified to improve the follow-up rate but decrease the details of specific outcomes. The results showed a high but biased follow-up rate, with more information obtained for people who graduated from the program. The residents with planned discharge showed excellent outcomes in terms of living situation, working situation, healthcare utilization, and low severity of current symptoms. High compliance with medication spoke to the program's philosophy of including the residents in the decision-making process. The dual challenges of heterogeneity of outcomes and difficulty in follow-up that limit efforts to document the value of psychiatric rehabilitation are discussed.

Comparative studies of H(2)O(2) detoxifying enzymes in green and greening barley seedlings under cadmium stress.

Cadmium(Cd) stress induced alterations in the activities of several representatives of the enzymatic antioxidant defense system such as guiacol peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were comparatively studied in green and greening barley seedlings that represent two different stages of plant development. Although roots were the main site of Cd accumulation, 1.5-3% of Cd was translocated into leaves and it induced oxidative damage which was indicated by the reduced chlorophyll and increased malondialdehyde content of the leaves. In roots of both types of seedlings exposed to various Cd concentrations, the APX activity was enhanced without any increase in the activity of POD. In leaves, however, elevated activities of both POD and APX could be observed. In roots of green seedlings at high concentration of Cd, the APX activity was reduced on the fourth day of culture but no inhibition was found in the POD activity. Leaf CAT which mainly represented the peroxisomal enzyme activity did not display any changes under Cd stress. Our results show that at both developmental stages barley seedlings exhibit a well-defined activity of the enzymatic antioxidant system, which operates differentially in roots and shoots subjected to Cd stress.

Traumas and other adverse life events in adolescents with alcohol abuse and dependence.

OBJECTIVE: Clinical observation suggests that adolescents with alcohol use disorders often have complex histories that include childhood maltreatment and other traumas. The aim of this study was to determine the relationships among adolescent alcohol use disorders and a broad range of traumas and adverse life events. METHOD: The subjects were 132 adolescents with alcohol dependence, 51 adolescents with alcohol abuse, and 73 adolescents recruited from the community as a control group. Trauma history was assessed by a semistructured interview and other adverse life events by questionnaire. RESULTS: Traumatic events reflecting interpersonal violence had occurred in many of the adolescents with alcohol dependence and abuse and few of the control adolescents. Adolescents with alcohol abuse or dependence, compared with control subjects, were 6 to 12 times more likely to have a physical abuse history and 18 to 21 times more likely to have a sexual abuse history. Sexual abuse was more common in females, and victimization by other violent acts was more common in males. Many other adverse life events were also significantly more common in the alcohol use disorder groups than in the control group, including having a close friend die, arguments within the family, and legal difficulties. CONCLUSIONS: These results demonstrate that trauma and other adverse life events are strongly associated with alcohol use disorders in adolescents. Clinical screening of adolescents with alcohol use disorders for a range of traumatic events is recommended.

Heterogeneity of adolescent alcoholism.

Adolescent alcoholics in treatment were classified into two broad clusters. The larger group consisted of youth who demonstrated behavioral dyscontrol and hypophoria, whereas the smaller group consisted of individuals with primarily negative affect. These two clusters differed with respect to age of first drug use, age of first substance abuse diagnosis, severity of substance use, behavior disturbance, and psychiatric disorder. Difficult temperament loaded on the negative affect factor. In a second study, it was observed that difficult childhood temperament led to a number of different first diagnoses in the adolescents. No specific psychiatric outcome followed a difficult temperament. These results illustrate the heterogeneity of the adolescent alcoholic population and suggest that there are many developmental pathways to this outcome where a predisposing risk factor is a difficult temperament makeup.

Neurobehavioral correlates of cholestatic and hepatocellular disease: differentiation according to disease specific characteristics and severity of the identified cerebral dysfunction.

Subjects with three different types of hepatic cirrhosis were compared on a battery of neuropsychological tests to determine if cerebral dysfunction varied according to the type of liver disease. It was found that the manifest cognitive impairments varied according to the type and etiology of the liver disease. These findings underscore the sensitivity of neuropsychological tests in the detection of hepatic encephalopathy, particularly in cases where there are no overt clinical signs or symptoms of neurologic disturbance. They also suggest that the nature and severity of the measurable neuropsychological abnormalities may vary within patient populations depending upon the etiology of the individual's liver disease and the specific pathophysiological mechanisms involved in its progression.

Cerebral CT scan abnormalities in cholestatic and hepatocellular disease and their relationship to neuropsychologic test performance.

Forty-nine nonalcoholic cirrhotic patients, on whom cranial CT scans were available, were administered a battery of neuropsychological tests. Although none of the subjects exhibited clinical signs or symptoms of hepatic encephalopathy, quantification of the CT scan image implicated cerebral edema and cortical atrophy. In addition numerous significant correlations were observed between the CT variables and neuropsychological test performance. The findings are conjectured to reflect previously unrecognized cerebral morphologic changes associated with chronic subclinical portal-systemic encephalopathy.

Perceptual field dependency: predisposing trait or consequence of alcoholism?

Perceptual field dependency often has been reported in alcoholics and frequently implicated as a predisposing trait in alcoholism. Comparing sons of alcoholics who are at high risk for alcoholism to sons of depressed fathers and control fathers (nonalcoholics without history of depression) revealed, however, no differences on the rod and frame test. These results do not support the hypothesis that field dependency is a risk factor in alcoholism.

Prevalence of behavior problems and mental health utilization among encopretic children: implications for behavioral pediatrics.

The psychiatric literature traditionally has viewed encopretic children as having severe and characteristic behavior problems. Current psychiatric views as expressed in the Diagnostic and Statistical Manual of Mental Disorders, 3rd Edition (DSM-III), and in the pediatric literature, however, tend to regard the behavior problems of encopretic children as being rather mild or secondary to the consequences of the encopresis itself. Using a behavioral checklist with empirically derived factors (the Child Behavior Checklist), 55 children presenting for medical treatment of their encopresis have been assessed. Numerous behavior problems are reported on parental ratings, but the overall severity of behavior problems in this group of children falls below that found in children with behavior problems who are referred for mental health services. Most children presenting for medical management of encopresis have not been seen or evaluated by mental health services. Encopresis is a disorder in which psychological evaluation and intervention by the pediatrician may be advisable.

Hepatic dysfunction and neuropsychological test performance in alcoholics with cirrhosis.

Fifteen alcoholic inpatients who had had a liver biopsy for medical reasons were given a series of laboratory tests and a neuropsychological test battery. Results indicated significant correlations between the biochemical measures of hepatic dysfunction and different aspects of cognitive test performance.

Neuropsychological capacity of Prader-Willi children: general and specific aspects of impairment.

Although most Prader-Willi syndrome children perform in the mentally retarded ranges on standardized IQ tests, it is not known if their cognitive impairments are global in nature or if they exhibit a particular pattern of strengths and weaknesses in their psychological capacities. To examine this question, a cohort of children suffering from Prader-Willi syndrome was administered a battery of neuropsychological tests. The results indicated that, relative to other cognitive capacities assessed, particularly severe deficits were noted on tasks that involved information processing using the auditory modality. No differences in cognitive capacity were found between children with a number 15 chromosome defect and those with a normal karyotype configuration. Based on these initial findings, it appears that the clinical diagnosis of Prader-Willi syndrome is more important than a karyotype configuration in understanding these youngsters' manifest cognitive deficits.

Neurological mechanisms underlying inheritance of alcoholism vulnerability.

Evidence accrued during the past two decades points to a genetic predisposition for alcoholism. The biological expression of the genetic vulnerability, however, is unknown. This discussion addresses the neurological manifestation of alcoholism vulnerability, including the biochemical, physiological and behavioral characteristics which appear to be associated with an increased risk for alcoholism.

Neuropsychological functioning in children with immunodeficiency disorders.

A small group of children with various types of immunodeficiency disorders, examined on a battery of neuropsychological and intellectual tests, was found to be impaired on perceptual speed, visuospatial sequencing, and visual attention span capacities when compared to a control group of closest aged siblings and normal children matched for age, education, and IQ. The results suggest that cerebral dysfunction may be associated with immunodeficiency disorders in children, although these findings are tentative since other factors, such as the effects of chronic illness, could not be excluded in this small cohort of patients.

Hyperactivity in sons of alcoholics.

In a group of delinquent adolescent boys, the sons of alcoholic fathers (N = 16) did not differ from the sons of nonalcoholic fathers (N = 25) on either the number or pattern of features characterizing hyperactivity-minimal brain dysfunction. The findings suggest that childhood hyperactivity cis not a risk factor specific to alcoholism.

Intellectual profiles and violent behavior in juvenile delinquents.

Hemispheric laterality, measured by the Wechsler Verbal IQ-Performance IQ differential score, in a large group of delinquents (N = 101) was not associated with violent-nonviolent behavior. Delinquents were, however, likely to be relatively more impaired on verbal than on nonverbal intellectual capacities.

Portal-systemic encephalopathy: neuropsychiatric manifestations.

Portal-systemic encephalopathy, which occurs as a consequence of cirrhosis of the liver, is associated with a variety of neuropsychiatric symptoms. Problems related to the differential diagnosis and medical management of portal-systemic encephalopathy, as compared to other more usual psychiatric disorders, are discussed. Also addressed are issues of particular concern to psychiatry, such as the use of pharmacotherapeutics and the rehabilitation of alcoholism, which are commonly seen in individuals with portal-systemic encephalopathy and liver disease, as well as in the general psychiatric patient population.

Static ataxia: a possible marker for alcoholism.

Adolescent sons of alcoholic, depressed, and normal fathers were compared on a measure of static ataxia, or body sway. It was found that the young males at risk for developing alcoholism were more ataxic than the sons of depressed fathers and normal controls. The possibility that static ataxia may be a premorbid marker for detecting males at heightened risk for alcoholism is discussed.

Neuropsychiatric sequelae of portal-systemic encephalopathy: a review.

Portal-systemic encephalopathy (PSE) is a common condition affecting individuals with chronic hepatic dysfunction. The clinical presentation of PSE often mimics a number of psychiatric disorders and, as discussed in the text, complicates the treatment of persons who suffer from both liver dysfunction and psychiatric illness. This review of the literature addresses the etiology, natural history and treatment of PSE. Special attention is devoted to clarifying the cognitive and affective manifestations of chronic low grade PSE.