ABSTRACT
The objective of this work is to synthesize and investigate the anticancer activity of a new series of sulfaquinoxaline derivatives by incorporating biologically active moieties (thiourethane, thiazole, imidazole, imidazopyrimidine, imidazopyrimido-pyrimidine, thienopyrimidine, benzopyrimidinone, benzothiazole, thiazole and pyridine moieties). All the newly synthesized compounds were evaluated for their in-vitro anticancer activity against human liver cell line (HEPG2). All the tested compounds showed comparable activity to that of the reference drug 5-fluorouracil (IC50=40 µM), and the most potent compounds were found to be compounds 4 and 17 (IC50=4.29 and 11.27 µM, respectively). On the other hand, the most potent compounds 4 and 17 were evaluated as radiosensitizing agents.
Subject(s)
Antineoplastic Agents/chemical synthesis , Radiation-Sensitizing Agents/chemical synthesis , Sulfonamides/chemical synthesis , Antineoplastic Agents/pharmacology , Cell Line, Tumor , Drug Screening Assays, Antitumor , Humans , Radiation-Sensitizing Agents/pharmacology , Sulfonamides/pharmacology , BenzenesulfonamidesSubject(s)
Oxazines/chemical synthesis , Quinolines/chemical synthesis , Alanine Transaminase/blood , Animals , Aspartate Aminotransferases/blood , Creatinine/blood , Liver Function Tests , Male , Oxazines/pharmacology , Quinolines/pharmacology , Rats , Rats, Wistar , Spectrophotometry, Infrared , Urea/bloodABSTRACT
Fatty acid compositions of twelve algal species from two different classes were determined. Three Chlorophyta species (Acetabularia calyculus, Cladophora sericoides and Dictyosphaeria cavernosa) and nine Phaeophyta species (Colpomenia sinuosa, Cystoseria trinodis, Dictyota cervicornis, Hormophysa triquetra, Padina gymnospora, Sargassum binderi, S. boveanum, S. denticulatum and S. heteromorpha) were investigated. Thirty-four fatty acids were identified. Myristic, palmitic, oleic, linoleic, eicosodienic and lignoceric acids were predominant in all examined algal species. Branched saturated fatty acids were also found in all investigated species except Cladophora sericoides.
Subject(s)
Eukaryota/chemistry , Fatty Acids/analysis , Chlorophyta/chemistry , Diatoms , Fatty Acids, Unsaturated/analysis , QatarABSTRACT
The fatty acid composition of eleven species of algae belonging to Rhodophyceae, collected from the coastal zones of Qatar, are reported. The major fatty acids identified were palmitic (16:0), myristic (14:0), oleic (18:1), eicosadienoic (20:2), linoleic (18:2), stearic (18:0) and hexadecaenoic acid (16:1). The fatty acids were characterized by the relatively high abundance of polyunsaturated acids, while the C18 unsaturated acids were appreciably more abundant than the C20 unsaturated acids.
Subject(s)
Eukaryota/analysis , Fatty Acids/analysis , QatarABSTRACT
Green onions (Allium cepa) were homogenized in a blender and extracted by normal extraction methods except that diethyl ether was used as the first extracting solvent. Different analytical procedures were used for the identification of the prostaglandins separated. TLC was applied using silica gel 60 F254 plates and a mixture of benzene, dioxane and acetic acid (20:10:1) as eluent, and the Rf values were compared with those of authentic samples. GC analysis on an SE 30 packed column and FID was applied; relative retention times of the onion extract components were measured and matched with authentic prostaglandin samples using cholesterol as an internal standard. GC-MS analyses using the same conditions adopted for GC analysis were conducted on a Finnigan MAT 112S instrument. Four peaks were identified. The prostaglandins identified were F1 alpha, E1, B1 and A2.
