ABSTRACT
Russet on apple fruits was caused by inoculation with isolates of Aureobasidium pullulans and Rhodotorula glutinis but not with Metschnikowia pulcherrima or two other unidentified yeasts, YT2 and YT17. Another unidentified yeast, YT5, caused russet in 1 of 2 years. Epiphytic survival of isolates on fruit was measured 3 months after inoculation. All isolates that caused russet survived epiphytically on fruit at harvest. With the exception of M. pulcherrima, nonrusseting isolates were not detected on the fruit at harvest. Conidia, hyphae, and chlamydospores characteristic of A. pullulans were observed in naturally russeted tissues of 14 different apple cultivars and Bartlett and Bosc pear collected from 13 orchards in New York state. A. pullulans was the most commonly isolated microorganism, and all A. pullulans isolates caused apple russet but varied in the severity of russet they caused. Isolates were identified using standard identification schemes. In addition, A. pullulans isolates were compared by generating fingerprints of restriction fragments of a PCR-amplified region of ribosomal DNA consisting of ITS-1, the 5.8S gene, and ITS-2.
