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2.
Biochem Biophys Res Commun ; 284(2): 326-30, 2001 Jun 08.
Article in English | MEDLINE | ID: mdl-11394880

ABSTRACT

It remains controversial whether deficiency of the Niemann-Pick C1 (npc1) protein results in altered cholesterol signaling at the endoplasmic reticulum (ER). In this report, we have measured the processed, nuclear form of sterol regulatory element binding protein (SREBP)-1 in livers of npc1 wild-type, heterozygous, and homozygous deficient mice, alone, and in combination with deficiencies of the low density lipoprotein receptor (LDLR) or the multiple drug resistant (mdr)1a, P-glycoprotein. Cleavage of SREBPs to activated forms normally occurs when the ER is deficient in cholesterol. A large decrease in processed SREBP-1 was evident in fasted npc1(-/-) mice and npc1(-/-), mdr1a(-/-) mice, with no decrease evident in npc1(-/-), LDLR(-/-) mice. These results suggest that the increase in cellular cholesterol which occurs in npc1(-/-) and in npc1(-/-), mdr1a(-/-) mice includes the sites responsible for cholesterol signaling, while the similar increase in cholesterol found in npc1(-/-), LDLR(-/-) mice does not.


Subject(s)
CCAAT-Enhancer-Binding Proteins/metabolism , Cholesterol/metabolism , DNA-Binding Proteins/metabolism , Endoplasmic Reticulum/metabolism , Proteins/metabolism , Receptors, LDL/deficiency , Transcription Factors , ATP Binding Cassette Transporter, Subfamily B/deficiency , ATP Binding Cassette Transporter, Subfamily B/genetics , ATP-Binding Cassette Transporters/genetics , Animals , Female , Heterozygote , Homozygote , Intracellular Signaling Peptides and Proteins , Liver/metabolism , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Mutant Strains , Niemann-Pick C1 Protein , Proteins/genetics , Receptors, LDL/genetics , Sex Factors , Signal Transduction/physiology , Sterol Regulatory Element Binding Protein 1
3.
J Biol Chem ; 276(13): 10314-9, 2001 Mar 30.
Article in English | MEDLINE | ID: mdl-11152466

ABSTRACT

Niemann-Pick type C (NPC) disease is characterized by an accumulation of cholesterol in most tissues and progressive neurodegeneration with the formation of neurofibrillary tangles. Neurofibrillary tangles are composed of paired helical filaments (PHF), a major component of which is the hyperphosphorylated tau. In this study we used NPC heterozygous and NPC homozygous mouse brains to investigate the molecular mechanism responsible for tauopathy in NPC. Immunoblot analysis using anti-tau antibodies (Tau-1, PHF-1, AT-180, and AT-100) revealed site-specific phosphorylation of tau at Ser-396 and Ser-404 in the brains of NPC homozygous mice. Mitogen-activated protein kinase, a potential serine kinase known to phosphorylate tau, was activated, whereas other serine kinases such as glycogen synthase kinase-3beta and cyclin-dependent kinase 5 were inactive. Morphological examination demonstrated that a number of neurons, the perikarya of which strongly immunostained with PHF-1, exhibited polymorphorous cytoplasmic inclusion bodies and multi-concentric lamellar-like bodies. Importantly, the accumulation of intracellular cholesterol in NPC mouse brains was determined to be a function of age. From these results we conclude that abnormal cholesterol metabolism due to the genetic mutation in NPC1 may be responsible for activation of the mitogen-activated protein kinase-signaling pathway and site-specific phosphorylation of tau in vivo, leading to tauopathy in NPC.


Subject(s)
MAP Kinase Signaling System , Mitogen-Activated Protein Kinases/metabolism , tau Proteins/metabolism , Age Factors , Alkaline Phosphatase/metabolism , Animals , Brain/enzymology , Brain/metabolism , Calcium-Calmodulin-Dependent Protein Kinases/metabolism , Cell Nucleus/metabolism , Cerebellum/enzymology , Cholesterol/metabolism , Cyclin-Dependent Kinase 5 , Cyclin-Dependent Kinases/metabolism , Cytoplasm/metabolism , Disease Models, Animal , Enzyme Activation , Glycogen Synthase Kinase 3 , Glycogen Synthase Kinases , Heterozygote , Homozygote , Hot Temperature , Immunoblotting , Lipid Metabolism , Liver/enzymology , Mice , Mice, Inbred BALB C , Microscopy, Electron , Mutation , Niemann-Pick Diseases/genetics , Phosphorylation , Purkinje Cells/metabolism , Serine/chemistry , Signal Transduction , Telencephalon/enzymology , Time Factors
4.
Life Sci ; 70(2): 131-42, 2001 Nov 30.
Article in English | MEDLINE | ID: mdl-11787939

ABSTRACT

Niemann-Pick type C (NPC) is a neurodegenerative disorder characterized by greatly altered somatic cholesterol metabolism. The NPC1 gene has recently been cloned and shown to have sequence homology to other sterol-sensing proteins. We have used a mouse model with a disrupted npc1 gene to study the effects of the cholesterol-mobilizing compound, 2-hydroxypropyl-beta-cyclodextrins (HPBCD), on the clinical course of this disorder. Treatment with two HPBCDs, with varying levels of 2-hydroxypropyl substitution, had effects in delaying neurological symptoms and in decreasing liver cholesterol storage while a third HPBCD was without effect. The ameliorating effect was not improved by longer exposure times (commencement of exposure in utero), however, it is not known if there is transplacental transfer of HPBCDs. The combination of HPBCD with probucol or nifedipine (which have previously been shown to lower liver cholesterol in this animal model) markedly decreased liver storage of unesterified cholesterol without altering the depressed levels of esterified cholesterol. The slight effects of the HPBCDs on neurological symptoms may be partially due to their apparent non-permeation of the blood-brain barrier (BBB). This non-permeation was assayed with radioactive tracers and was also present in the mdr1a knockout mice which have greatly increased BBB permeability for many drugs. Intrathecal delivery of HPBCD by an Alzet osmotic minipump did not improve its efficacy in ameliorating neurological symptoms.


Subject(s)
Anticholesteremic Agents/therapeutic use , Cyclodextrins/therapeutic use , Niemann-Pick Diseases/drug therapy , beta-Cyclodextrins , 2-Hydroxypropyl-beta-cyclodextrin , Animals , Anticholesteremic Agents/administration & dosage , Anticholesteremic Agents/pharmacokinetics , Ataxia/drug therapy , Ataxia/physiopathology , Blood-Brain Barrier/drug effects , Blood-Brain Barrier/physiology , Brain/drug effects , Brain/metabolism , Cholesterol/analysis , Cyclodextrins/administration & dosage , Cyclodextrins/pharmacokinetics , Disease Models, Animal , Dose-Response Relationship, Drug , Drug Therapy, Combination , Female , Injections, Intraperitoneal , Injections, Spinal , Intracellular Signaling Peptides and Proteins , Liver/drug effects , Liver/metabolism , Male , Mice , Mice, Knockout , Niemann-Pick C1 Protein , Niemann-Pick Diseases/genetics , Niemann-Pick Diseases/physiopathology , Nifedipine/therapeutic use , Probucol/therapeutic use , Proteins/genetics , Tremor/drug therapy , Tremor/physiopathology
5.
Am J Med Genet ; 95(3): 204-7, 2000 Nov 27.
Article in English | MEDLINE | ID: mdl-11102924

ABSTRACT

The recent finding that a subset of patients with Rothmund-Thomson syndrome (RTS) have mutations of a helicase gene has prompted reexamination of the phenotypes of individuals diagnosed with this disorder. We report on two patients with variable presentations of RTS. Initial presenting symptoms included growth deficiency and absent thumbs in one patient and osteogenic sarcoma and poikiloderma in the second patient. The growth-deficient patient was diagnosed with growth hormone deficiency and had a subnormal response to growth hormone supplementation. Neither malformations nor growth deficiency were present in the patient with osteogenic sarcoma, and her only other manifestation of RTS was poikiloderma. The diagnosis of RTS should be considered in all patients with osteogenic sarcoma, particularly if associated with skin changes.


Subject(s)
Rothmund-Thomson Syndrome/complications , Abnormalities, Multiple/pathology , Child , Erythema/etiology , Erythema/pathology , Follow-Up Studies , Humans , Infant, Newborn , Male , Neoplasms, Second Primary , Osteosarcoma/etiology , Rothmund-Thomson Syndrome/pathology , Skin Diseases, Genetic/etiology , Skin Diseases, Genetic/pathology
6.
Mamm Genome ; 11(9): 774-8, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10967137

ABSTRACT

Mouse Niemann-Pick disease type C1 (npc1), formerly designated spm (sphingomyelinosis), is an autosomal recessive lipid storage disorder. We generated a high-resolution linkage map in the 2.24-cM npc1 critical region by typing eight polymorphic markers in 2322 meioses (948 of these were previously reported). A minimal set of overlapping yeast artificial chromosomes (YACs) had previously been assembled (Hsu and Erickson 2000). The YAC 313-B-8, which covered this whole region, has been used to construct cosmid libraries. Three cosmid contigs were built, and one of them contained the npc1 locus. Two (CA)(n) microsatellites were identified, and the one new one was characterized, from the YAC-derived cosmids. The most proximal cosmid contig overlaps with markers near twirler (Tw). Both the physical map and genetic linkage map have been integrated to study the recombination frequencies in this particular region of the mouse genome, and recombination suppression due to the heterozygous insertion of DNA was suggested.


Subject(s)
Chromosome Mapping , Proteins/genetics , Recombination, Genetic/genetics , Retroelements , Animals , Chromosomes, Artificial, Yeast , Contig Mapping , Cosmids , Dinucleotide Repeats , Female , Intracellular Signaling Peptides and Proteins , Male , Mice , Mice, Inbred BALB C , Mice, Inbred DBA , Microsatellite Repeats , Mutagenesis, Insertional , Mutation , Niemann-Pick C1 Protein , Physical Chromosome Mapping , Polymorphism, Genetic , Sequence Tagged Sites
7.
Ann Neurol ; 47(5): 583-8, 2000 May.
Article in English | MEDLINE | ID: mdl-10805328

ABSTRACT

Fumaric aciduria (fumaric acidemia, fumarase deficiency) is a rare inborn error of metabolism caused by deficient activity of fumarate hydratase, one of the constituent enzymes of the Krebs tricarboxylic acid cycle. We describe the clinical and imaging features of this disease arising from a consanguineous pedigree in 8 patients in the southwestern United States. Thirteen patients have been previously described in the medical literature. Our patients presented with an early infantile encephalopathy with profound developmental retardation and hypotonia, and most experienced seizures. Previously unreported characteristics described here include structural brain malformations, dysmorphic facial features, and neonatal polycythemia. Magnetic resonance imaging showed multiple abnormalities, including diffuse polymicrogyria, decreased cerebral white matter, large ventricles, and open opercula. Fumaric aciduria should be included in the differential diagnosis of inborn errors of metabolism that cause cerebral malformations and dysmorphic features. The possibility that inborn errors of energy metabolism may cause structural malformations deserves increased recognition.


Subject(s)
Fumarate Hydratase/deficiency , Metabolism, Inborn Errors/diagnosis , Metabolism, Inborn Errors/enzymology , Abnormalities, Multiple , Brain/abnormalities , Child , Child, Preschool , Consanguinity , Female , Fumarate Hydratase/urine , Humans , Infant , Intellectual Disability/complications , Magnetic Resonance Imaging , Male , Metabolism, Inborn Errors/epidemiology , Muscle Hypotonia/complications , Pedigree , Polycythemia/complications , Seizures/complications , United States/epidemiology
8.
J Lipid Res ; 41(5): 673-87, 2000 May.
Article in English | MEDLINE | ID: mdl-10787428

ABSTRACT

Niemann-Pick type C (NPC) disease is characterized by an accumulation of cholesterol and other lipids in the lysosomal compartment. In this report, we use subcellular fractionation and microscopy to determine the localization of the murine Niemann-Pick C1 (NPC1) protein. Fractionation of mouse liver homogenates indicates that some NPC1 cosediments with lysosome-associated membrane protein 1 (LAMP1)-containing membranes. However, a significant amount of NPC1 is also found in membranes that do not contain LAMP1. Moreover, fractionation of liver membranes and fibroblasts in the presence of a nonionic detergent showed that a fraction of NPC1 cosediments with caveolin-1 in rafts. Immunofluorescence microscopy of cultured mouse fibroblasts showed that NPC1 is found in two morphologically distinct structures. The first population is characterized by large punctate structures that do not colocalize with major organelle protein markers, but do colocalize with filipin and a small fraction of caveolin-1. Examination of these large NPC1-containing compartments using electron microscopy shows that these structures contain extensive internal membranes. The second population is represented by smaller, more diffuse structures, a fraction of which colocalize with LAMP1-positive compartments. Incubation of fibroblasts with low density lipoprotein (LDL) increases colocalization of NPC1 with LAMP1-containing compartments. This colocalization can be further enhanced by treating fibroblasts with progesterone or chloroquine. The results indicate that NPC1 is associated with an unique vesicular compartment enriched with cholesterol and containing caveolin-1, and that NPC1 cycles to LAMP1-positive compartments, presumably to facilitate the processing of LDL-derived cholesterol.


Subject(s)
Niemann-Pick Diseases/metabolism , Proteins/metabolism , Amino Acid Sequence , Animals , Antibodies , Cell Compartmentation , Cell Fractionation , Centrifugation, Density Gradient , Cholesterol/metabolism , Female , Fibroblasts/metabolism , Intracellular Signaling Peptides and Proteins , Liver/metabolism , Liver/ultrastructure , Male , Mice , Mice, Inbred BALB C , Mice, Mutant Strains , Microscopy, Fluorescence , Microscopy, Immunoelectron , Molecular Sequence Data , Niemann-Pick C1 Protein , Niemann-Pick Diseases/genetics , Proteins/genetics , Proteins/immunology , Rabbits
9.
J Inherit Metab Dis ; 23(1): 54-62, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10682308

ABSTRACT

Niemann-Pick type C (NPC) is a neurodegenerative disorder with somatically altered cholesterol metabolism. The NPC1 gene has recently been cloned and shown to have sequences shared with known sterol-sensing proteins. We have used a mouse model with a disrupted Npc1 gene to study two cholesterol-lowering drugs (nifedipine and probucol) and the effects of introducing a null mutation in the low-density lipoprotein receptor (LDLR). Although these treatments significantly ameliorated liver cholesterol storage, little effect on the onset of neurological symptoms was found.


Subject(s)
Cholesterol/metabolism , Niemann-Pick Diseases/drug therapy , Nifedipine/therapeutic use , Probucol/therapeutic use , Receptors, LDL/physiology , Animals , Blood-Brain Barrier , Mice , Mice, Inbred BALB C , Mice, Knockout , Niemann-Pick Diseases/genetics
10.
Biochim Biophys Acta ; 1453(2): 193-206, 1999 Feb 24.
Article in English | MEDLINE | ID: mdl-10036317

ABSTRACT

We have previously demonstrated (1) an increased expression of caveolin-1 in murine heterozygous and homozygous Niemann-Pick type C (NPC) livers, and (2) an increased concentration of unesterified cholesterol in a detergent insoluble caveolae-enriched fraction from homozygous livers. To define further the relationship between caveolin-1 function and the cholesterol trafficking defect in NPC, we examined the expression and distribution of additional caveolar and signal transduction proteins. The expression of annexin II was significantly increased in homozygous liver homogenates and the Triton X-100 insoluble floating fraction (TIFF). Phosphoamino acid analysis of caveolin-1 and annexin II from the homozygous TIFF demonstrated an increase in serine and tyrosine phosphorylation, respectively. To determine the basis for increased phosphorylation of these proteins, the expression and distribution of several protein kinases was examined. The expression of PKCalpha, PKCzeta and pp60-src (protein kinases) were significantly increased in both heterozygous and homozygous liver homogenates, while PKCdelta was increased only in homozygous livers. Of the protein kinases analyzed, only CK IIalpha was significantly enriched in the heterozygous TIFF. Finally, the concentration of diacylglycerol in the homozygous TIFF was significantly increased and this elevation may modulate PKC distribution and function. These results provide additional evidence for involvement of a caveolin-1 containing cellular fraction in the pathophysiology of NPC and also suggest that the Npc1 gene product may directly or indirectly, regulate the expression and distribution of signaling molecules.


Subject(s)
Annexin A2/metabolism , Caveolins , Liver/metabolism , Membrane Proteins/metabolism , Protein Kinases/metabolism , Proteins/genetics , Animals , Annexin A2/biosynthesis , Caveolin 1 , Cholesterol/analysis , Diglycerides/analysis , Disease Models, Animal , Female , In Vitro Techniques , Intracellular Signaling Peptides and Proteins , Male , Membrane Proteins/biosynthesis , Mice , Mice, Inbred BALB C , Niemann-Pick C1 Protein , Niemann-Pick Diseases/genetics , Octoxynol , Phospholipids/analysis , Phosphorylation , Protein Kinases/biosynthesis , Signal Transduction/genetics , Solubility
11.
Hum Genet ; 103(2): 162-7, 1998 Aug.
Article in English | MEDLINE | ID: mdl-9760199

ABSTRACT

Four patients with primapterinuria, postulated to be due to pterin-4alpha-carbinolamine dehydratase (PCD) deficiency, were diagnosed by biochemical and DNA analysis. All four patients presented in the neonatal period with hyperphenylalaninemia, and elevated neopterin and decreased biopterin levels in the urine. These symptoms are common to 6-pyruvoyltetrahydropterin synthase deficiency and thus there is a danger of misdiagnosis. In addition, all four patients had elevated urinary excretion of primapterin (7-biopterin), the only persistent biochemical abnormality. Analysis of fibroblast DNA from the patients identified the following mutations in the PCBD gene: one patient homozygous for the missense mutation E96K and one homozygous for the nonsense mutation Q97X, both in exon 4; one compound heterozygote with the mutations E96K and Q97X; and one patient with two different homozygous mutations: E26X in exon 2 and R87Q in exon 4. In two families, the parents were investigated and found to be obligate heterozygotes for particular mutations. One sibling was found to be unaffected. These results further substantiate the idea that primapterinuria is associated with mutations in the PCBD gene.


Subject(s)
Amino Acid Metabolism, Inborn Errors/enzymology , Hydro-Lyases/genetics , Mutation , Phenylalanine/metabolism , Phenylketonurias/enzymology , Amino Acid Metabolism, Inborn Errors/genetics , Female , Humans , Hydro-Lyases/metabolism , Infant, Newborn , Male , Phenylketonurias/genetics , Pterins/urine
12.
Biochim Biophys Acta ; 1361(3): 272-80, 1997 Oct 24.
Article in English | MEDLINE | ID: mdl-9375801

ABSTRACT

Niemann-Pick type C (NPC) is an autosomal recessive disease characterized by impaired cholesterol homeostasis due to a defect in the intracellular transport of unesterified cholesterol. While accumulation of lysosomal cholesterol is the most apparent microscopic finding, cholesterol has also been shown to accumulate in the trans-cisternae of the Golgi apparatus. Caveolin-1, a cholesterol-binding protein that cycles between the Golgi apparatus and the plasma membrane, has been hypothesized to participate in cholesterol transport. Using the BALB/c murine model for NPC disease, we found that the expression of caveolin-1 in total liver homogenates from heterozygous and homozygous affected animals was altered. Immunoblot analysis of liver homogenates from heterozygous mice revealed that caveolin-1 is significantly (p < 0.005) elevated, 4.9 fold, compared to normal mice. Total liver homogenates from homozygous affected mice also had a significant (p < 0.05) increase in caveolin-1 expression, 1.6 fold, compared to normal mice. Immunohistochemical staining of liver cross-sections revealed that the increased caveolin-1 was localized to sinusoidal endothelial cells in heterozygous mice. The Triton insoluble floating fraction (TIFF) was isolated using liver from each genotype and analyzed for caveolin-1 expression. Caveolin-1 in the TIFF from heterozygous mice was significantly (p < 0.01) elevated, 1.8 fold, compared to normal and homozygous affected animals; normal and homozygous affected animals, however, were not significantly different from each other. The TIFF isolated from homozygous affected mice revealed a 15 fold increase in unesterified cholesterol compared to the TIFF isolated from heterozygous and normal mice. In summary, these findings demonstrate an altered expression of caveolin-1 in liver from heterozygous and homozygous NPC mice and increased concentration of cholesterol from TIFF in homozygous affected NPC mice. The identification of these alterations in the TIFF suggests involvement of detergent insoluble membrane structures, possibly caveolae and/or detergent insoluble glycosphingolipid-enriched complexes (DIGs), in the cholesterol trafficking defect in this disorder.


Subject(s)
Caveolins , Cholesterol/metabolism , Liver/metabolism , Membrane Proteins/genetics , Niemann-Pick Diseases/metabolism , Animals , Biological Transport , Caveolin 1 , Immunohistochemistry , Mice , Mice, Inbred BALB C
13.
Biochem Biophys Res Commun ; 236(1): 189-93, 1997 Jul 09.
Article in English | MEDLINE | ID: mdl-9223450

ABSTRACT

Human Niemann-Pick type II fibroblasts, which encompass the panethnic type C (NPC) and Nova Scotia Acadian type D (NPD) variants, exhibit altered expression of caveolin-1 protein when examined by immunoblotting using an anti-caveolin-1 monoclonal antibody. Unexpectedly, caveolin-1 in heterozygous fibroblasts was significantly elevated as much as 10-fold compared to caveolin-1 in normal and homozygous affected fibroblasts. Homozygous NPC fibroblasts expressed caveolin-1 levels similar to those in normal fibroblasts, while the expression of caveolin-1 in homozygous NPD fibroblasts was slightly elevated. Northern analysis indicates that normal fibroblasts and NPC heterozygous fibroblasts have similar amounts of caveolin-1 mRNA, while NPC homozygous fibroblasts have significantly less caveolin-1 mRNA. In contrast, heterozygous and homozygous NPD fibroblasts exhibit increased levels of caveolin-1 mRNA. These novel findings suggest that caveolin-1 containing subcellular structures are involved in the pathophysiology of Niemann-Pick type II disease. Furthermore, altered caveolin-1 protein expression may serve as a useful marker for the diagnosis of carriers of NPC or NPD.


Subject(s)
Caveolins , Membrane Proteins/biosynthesis , Niemann-Pick Diseases/metabolism , Caveolin 1 , Cells, Cultured , Fibroblasts/metabolism , Heterozygote , Humans , Membrane Proteins/genetics , Niemann-Pick Diseases/genetics
15.
Am J Med Genet ; 58(2): 125-7, 1995 Aug 28.
Article in English | MEDLINE | ID: mdl-8533802

ABSTRACT

Distal arthrogryposis IIB is characterized by contractures of the distal joints (especially of the fingers and toes) and ptosis. We recently encountered a father and son with these manifestations. The father was reported 54 years ago as a case of amyoplasia congenita (arthrogryposis multiplex congenita). Both father and son have distal joint contractures, most severe in the hands and feet, as well as ptosis and ophthalmoplegia. In addition, these patients have an unusual distribution of hair loss, and conical teeth. Whether these latter findings are related to the type of distal arthrogryposis present in this family is not known. In spite of their physical limitations both father and son have maintained an active life-style.


Subject(s)
Arthrogryposis/genetics , Adult , Follow-Up Studies , Hair/abnormalities , Humans , Male , Middle Aged , Tooth Abnormalities/genetics
16.
Am J Hum Genet ; 57(1): 34-9, 1995 Jul.
Article in English | MEDLINE | ID: mdl-7611293

ABSTRACT

Cystathionine beta-synthase (CBS) deficiency is an autosomal recessive disorder characterized by homocystinuria and multisystem clinical disease. Patients responsive to pyridoxine usually have a milder clinical phenotype than do nonresponsive patients, and we studied the molecular pathology of this disorder in an attempt to understand the molecular basis of the clinical variation. We previously reported a T833C transition in exon 8 causing a substitution of threonine for isoleucine at codon 278 (I278T). By PCR amplification and sequencing of exon 8 from genomic DNA we have now detected the I278T mutation in 7 of 11 patients with in vivo pyridoxine responsiveness and in 0 of 27 pyridoxine-nonresponsive patients. Two pyridoxine-responsive patients are homozygous and five are heterozygous for I278T. We have now observed the I278T mutation in 41% (9 of 22) of the independent alleles in pyridoxine-responsive patients of varied ethnic backgrounds. In two of the compound heterozygotes we identified a novel mutation (G139R and E144K) in the other allele. The finding that the two patients who are homozygous for I278T have only ectopia lentis and mild bone demineralization suggests that this mutation is associated with both in vivo pyridoxine responsiveness and mild clinical disease. Compound heterozygous patients who have one copy of this missense mutation are likely to retain some degree of pyridoxine responsiveness.


Subject(s)
Cystathionine beta-Synthase/genetics , Homocystinuria/genetics , Point Mutation , Pyridoxine/pharmacology , Adult , Base Sequence , Cell Line , Child , DNA Mutational Analysis , Exons , Female , Humans , Male , Middle Aged , Molecular Sequence Data , Phenotype , Polymerase Chain Reaction , Polymorphism, Single-Stranded Conformational
17.
Eur J Pediatr ; 154(7 Suppl 2): S28-32, 1995.
Article in English | MEDLINE | ID: mdl-7671960

ABSTRACT

Analysis of both the human and rat galactose-1-phosphate uridyltransferase (GALT) genes reveal 5' regulatory consensus sequences suggestive of a housekeeping gene. This is in accord with the finding of GALT activity in all tissues. However, the complications seen in galactosemia, in particular ovarian dysfunction and verbal dyspraxia, suggest organ-specific sensitivity to lack of GALT activity. Analysis of steady-state GALT mRNA and specific activity levels in adult rat organs reveals a marked difference between organs that correlates with the degree of organ dysfunction in humans with galactosemia. The organ variation in GALT mRNA and activity thus appears to be due to genetic regulation. Discernment of the pathophysiologic basis for the organ-specific complications requires an understanding of the basis for the differences in organ regulation. The present state of knowledge of the regulation of the Leloir enzymes in general, GALT in particular, from prokaryotes to mammals is discussed.


Subject(s)
UTP-Hexose-1-Phosphate Uridylyltransferase/genetics , Animals , Gene Expression Regulation, Enzymologic , Humans , Rats
19.
Pediatr Res ; 34(4): 416-9, 1993 Oct.
Article in English | MEDLINE | ID: mdl-8255669

ABSTRACT

To assess the role of genetic regulation as a modulating factor in the variability of rat tissue galactose-1-phosphate uridyltransferase (GALT) sp act, we have determined steady state GALT mRNA and sp act in rat liver during postnatal development. Steady state GALT mRNA levels increase from birth to d 5 and subsequently decrease toward adult levels. GALT sp act mirrors the mRNA pattern. A survey of steady state mRNA and GALT sp act of several adult rat tissues revealed marked tissue differences with a good correlation of the two parameters. Liver had the highest GALT mRNA and sp act; kidney, ovary, and heart had similar but lower mRNA and sp act; skeletal muscle and testes had the least GALT mRNA and enzyme sp act. These findings suggest that genetic regulation is important in the variable expression of GALT tissue sp act.


Subject(s)
Aging/metabolism , Gene Expression Regulation, Enzymologic , Liver/enzymology , RNA, Messenger/metabolism , UTP-Hexose-1-Phosphate Uridylyltransferase/metabolism , Animals , Blotting, Northern , Female , Kidney/enzymology , Liver/growth & development , Lung/enzymology , Male , Muscles/enzymology , Myocardium/enzymology , Organ Specificity , Ovary/enzymology , RNA, Messenger/biosynthesis , Rats , Rats, Sprague-Dawley , Testis/enzymology , UTP-Hexose-1-Phosphate Uridylyltransferase/biosynthesis
20.
DNA Seq ; 3(5): 311-8, 1993.
Article in English | MEDLINE | ID: mdl-8400361

ABSTRACT

Galactosemia is an inborn error of metabolism in humans due to deficient activity of the enzyme galactose-1-phosphate uridyl-transferase (GALT). Using a 1.3 kb Bam HI restriction fragment of the human GALT cDNA as a probe, three cDNAs, 786 bp, 265 bp and 1.4 kb were isolated from a rat liver cDNA library. The total rat GALT coding sequence obtained from these three clones was 1051 nt. Repeat cDNA library screening failed to isolate cDNA clones with additional 5'-coding sequence. Using the 786 bp cDNA as a probe, three genomic clones, RGG1, RGG2, and RGG3 were isolated from a rat genomic library. RGG2 and RGG3 contained additional 5'-coding sequence, missing from the cDNA clones, which was identified by comparison with the human cDNA sequence. The rat GALT sequence, from transcription start site to the polyadenylation tail, is 1254 nt and is divided into 11 exons which span over 3.5 kb of genomic sequence. Primer extension identified the transcription start site as 17 nt 5' to the translation start site. Rat GALT is 379 amino acids long, identical to human GALT, with a derived molecular weight of 43,312 Da similar to the 43,360 Da weight for human GALT. The derived rat amino acid sequence is 90% identical to the human sequence. The isolation of rat GALT genomic sequence will facilitate future investigations of the regulation of expression of the rat GALT gene.


Subject(s)
UTP-Hexose-1-Phosphate Uridylyltransferase/biosynthesis , UTP-Hexose-1-Phosphate Uridylyltransferase/genetics , Amino Acid Sequence , Animals , Base Sequence , Blotting, Southern , Cloning, Molecular , Exons , Gene Library , Genetic Variation , Humans , Introns , Molecular Sequence Data , Oligodeoxyribonucleotides , Rats , Restriction Mapping , Sequence Deletion , Sequence Homology, Amino Acid , Sequence Homology, Nucleic Acid , Transcription, Genetic
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