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1.
Gene ; 506(2): 423-8, 2012 Sep 15.
Article in English | MEDLINE | ID: mdl-22759518

ABSTRACT

Australian marsupials are unique fauna that have evolved and adapted to unique environments and thus it is likely that their detoxification systems differ considerably from those of well-studied eutherian mammals. Knowledge of these processes in marsupials is therefore vital to understanding the consequences of exposure to xenobiotics. Cytochromes P450 (CYPs) are critically important in the oxidative metabolism of a diverse array of both xenobiotics and endogenous substrates. In this study we have cloned and characterized CYP3A70, the first identified member of the CYP3A gene subfamily from Eastern gray kangaroo (Macropus giganteus). A 1665 base pair kangaroo hepatic CYP3A complete cDNA, designated CYP3A70, was cloned by reverse transcription-polymerase chain reaction approaches, which encodes a protein of 506 amino acids. The CYP3A70 cDNA shares approximately 71% nucleotide and 65% amino acid sequence homology to human CYP3A4 and displays high sequence similarity to other published mammalian CYP3As from human, monkey, cow, pig, dog, rat, rabbit, mouse, hamster, and guinea pig. Transfection of the CYP3A70 cDNAs into 293T cells resulted in stable cell lines expressing a CYP3A immuno-reactive protein that was recognized by a goat anti-human CYP3A4 polyclonal antibody. The anti-human CYP3A4 antibody also detected immunoreactive proteins in liver microsomes from all test marsupials, including the kangaroo, koala, wallaby, and wombat, with multiple CYP3A immunoreactive bands observed in kangaroo and wallaby tissues. Relatively, very low CYP catalytic activity was detected for the kangaroo CYP3A70 cDNA-expressed proteins (19.6 relative luminescent units/µg protein), which may be due to low protein expression levels. Collectively, this study provides primary molecular data regarding the Eastern kangaroo hepatic CYP3A70 gene and enables further functional analyses of CYP3A enzymes in marsupials.


Subject(s)
Cytochrome P-450 CYP3A/chemistry , Cytochrome P-450 CYP3A/genetics , Gene Expression Regulation , Animals , Cell Line , Cloning, Molecular , DNA Primers/genetics , DNA, Complementary/metabolism , Female , Humans , Liver/metabolism , Macropodidae , Male , Marsupialia/genetics , Models, Genetic , Protein Isoforms , Rats , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction/methods , Species Specificity
2.
Gene ; 2011 Aug 18.
Article in English | MEDLINE | ID: mdl-21888957

ABSTRACT

This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy.

3.
Article in English | MEDLINE | ID: mdl-20826229

ABSTRACT

Cytochromes P450 (CYPs) are critically important in the oxidative metabolism of a diverse array of xenobiotics and endogenous substrates. We have previously reported the cloning and characterisation of the koala CYP4A15, the first reported member of the CYP4 family from marsupials, and have demonstrated important species differences in CYP4A activity and tissue expression. In the present study, the cloning of CYP4B1 in the wallaby (Macropus eugenii) and their expression across marsupials is described. Rabbit anti-mouse CYP4B1 antibody detected immunoreactive proteins in lung and liver microsomes from all test marsupials, with relative weak signal detected from the koala, suggesting a species-specific expression. Microsomal 2-aminofluorene bio-activation (a CYP4B1 marker) in wallaby lung was comparable to that of rabbit, with significant higher activities detected in wallaby liver and kidneys compared to rabbit. A 1548bp wallaby lung CYP4B complete cDNA, designated CYP4B1, which encodes a protein of 510 amino acids and shares 72% nucleotide and 69% amino acid sequence identity to human CYP4B1, was cloned by polymerase chain reaction approaches. The results demonstrate the presence of wallaby CYP4B1 that shares several common features with other published CYP4Bs; however the wallaby CYP4B1 cDNA contains four extra amino acid residues at the NH2-terminal, a fundamentally conserved transmembrane anchor of all eukaryote CYPs.


Subject(s)
Aryl Hydrocarbon Hydroxylases/metabolism , Lung/enzymology , Macropodidae/metabolism , Amino Acid Sequence , Animals , Aryl Hydrocarbon Hydroxylases/genetics , DNA, Complementary/isolation & purification , Fluorenes/metabolism , Immunoblotting , Kidney/enzymology , Liver/enzymology , Microsomes/enzymology , Molecular Sequence Data , Rabbits
4.
Hum Mutat ; 24(2): 185, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15241802

ABSTRACT

Two novel mutations of the human CLCN1 chloride channel gene, c.592C>G (p.L198V) and c.2255A>G (p.K752R), are described, occurring coincidentally in the one myotonic patient. These individual mutations and a construct with both mutations in the one cDNA were transcribed and expressed in Xenopus oocytes where channel gating parameters were extracted from chloride currents recorded under voltage clamp. We found that the p.L198V mutation has its major effects on the common (or slow) gate of the chloride channel, as do other dominant ClC-1 mutations, and may therefore be causative of the patient's symptoms (when co-expressed with wild-type human ClC-1, the p.L198V mutation exerts a dominant negative effect on common gating) but the p.K752R mutation appears to be innocuous and may be a benign polymorphism. A third mutant, the recently described c.2795C>T (p.P932L), was expressed in HEK 293 cells. Despite the severity of the disease associated with this mutation, chloride currents in cells expressing p.P932L were not significantly different from those of cells expressing wild-type ClC-1.


Subject(s)
Chloride Channels/genetics , Mutation/genetics , Myotonia Congenita/genetics , Black or African American/genetics , Animals , Child , Child, Preschool , Chloride Channels/physiology , Cloning, Molecular/methods , Female , Gene Expression Regulation/genetics , Humans , Ion Channel Gating/genetics , Kidney/chemistry , Kidney/cytology , Kidney/embryology , Kidney/metabolism , Male , Myotonia Congenita/pathology , Oocytes/chemistry , Oocytes/metabolism , Patch-Clamp Techniques/methods , Xenopus/genetics
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