Assessing Social Connectedness Among Persons with Schizophrenia: Psychometric Evaluation of the Perceived Social Connectedness Scale.

The purpose of this study was a psychometric evaluation of the 4-item perceived social connectedness (PSC) scale. The study analyzed secondary data from a project that assessed physical, behavioral, and social health characteristics of adults with serious mental illness receiving integrated services at community mental health centers (CMHs). The current sample comprised those diagnosed with schizophrenia attending these CMHs (N = 146). Most participants were African-American males who receive disability benefits with Medicaid as health insurance. The sample self-reported low-to-moderate levels of social connectedness, daily functioning, and symptom severity. Factor analysis of the PSC scale revealed one dimension, accounting for 66% of total variance, with strong item loadings. Reliability coefficients indicated sufficient scale internal consistency. Construct validity was suggested via the PSC scale's directional, significant convergence with daily functioning and symptom severity. Implications include the application of the PSC scale for this socioeconomically disadvantaged population that customarily lacks meaningful social networks.

Comparative sensitivity of solid phase versus PEG enhancement assays for detection and identification of RBC antibodies.

Blood banks require a sensitive, specific, and efficient method to detect clinically significant RBC antibodies. Solid phase antibody screening methods are popular due to high sensitivity and automation. However, the high degree of reactivity detects "false positive" antibodies of questionable clinical significance leading to additional testing. We studied positive rates of Capture-R vs. PEG methods and categorized RBC antibodies identified by initial test results of 33,564 consecutive samples by Capture-R method. Capture-R was positive in 1,084/33,564 (3.2%) of samples. Using PEG as our "gold standard", PEG confirmed true positivity (i.e., > or = 1 cell reacting) in 710 Capture-R positive samples (65.5%); 374 Capture-R positive samples (34.5%) did not react in PEG (i.e., false positives). Of the 710 samples with true positivity, only 510 showed clinically significant alloantibodies. Using PEG as our "gold standard", only 2/3 of reactions by Capture-R were considered true positives. Because of ease and automation, Capture-R is popular as a screening test, but a more specific method may be helpful in order to identify truly significant alloantibodies.

A red blood cell autoantibody with mimicking anti-E specificity.

BACKGROUND: Uncommonly, antibodies that appear to exhibit antigenic specificity on red blood cell (RBC) panels fail to maintain specificity following alloadsorption (i.e., they mimic antigenic specificity). Understanding both the pitfalls and the proper pathways to establish the diagnosis and to interpret the clinical significance of these mimicking antibodies is important for patient management. CASE REPORT: A 68-year-old woman was admitted with dyspnea, anemia, bilateral pulmonary emboli, and metastatic ovarian cancer. Blood bank evaluation identified anti-E reactivity in the patient's plasma sample and a positive direct antiglobulin test (DAT). RESULTS: The DAT was positive for immunoglobulin G and negative for C3b. An eluate of the RBCs showed E-antigen specificity on a RBC antibody panel. Repeat serologic testing with RBC antibody panels with adsorbed patient plasma showed removal of apparent anti-E reactivity with either E-antigen-positive or E-antigen-negative RBC stroma. CONCLUSION: A mimicking autoantibody with apparent E-antigen specificity was identified in the plasma sample of a woman with newly diagnosed ovarian cancer. Despite their relative low frequency, mimicking antibodies, whether auto- or alloantibodies, may interfere with the timely issuance of compatible blood products and may confuse laboratory and clinical staff. Determining the clinical significance of the antibody, by taking into account the RBC phenotype of the patient and the antigen prevalence in the general population, guides the extent of workup required to best utilize resources while assuring patient safety.