Poly(3-hydroxybutyrate) production using supplemented corn-processing byproducts through Cupriavidus necator via solid-state fermentation: Cultivation on flask and bioreactor scale.

The widespread adoption of Poly(3-hydroxybutyrate) (PHB) encounters challenges due to its higher production costs compared to conventional plastics. To overcome this obstacle, this study investigates the use of low-cost raw materials and optimized production methods. Specifically, food processing byproducts such as corn germ and corn bran were utilized as solid substrates through solid-state fermentation, enriched with molasses and cheese whey. Employing the One Factor at a Time technique, we examined the effects of substrate composition, temperature, initial substrate moisture, molasses, and cheese whey on PHB production at the flask scale. Subsequently, experiments were conducted at the bioreactor scale to evaluate the influence of aeration. In flask-scale experiments, the highest PHB yield, reaching 4.1 (g/kg Initial Dry Weight Substrate) (IDWS) after 72 hours, was achieved using a substrate comprising a 1:1 mass ratio of corn germ to corn bran supplemented with 20 % (v/w) cheese whey. Furthermore, PHB production in a 0.5-L packed-bed bioreactor yielded a maximum of 8.4 (g/kg IDWS), indicating a more than 100 % increase in yield after 72 hours, with optimal results achieved at an aeration rate of 0.5 l/(kg IDWS. h).

Enhanced biodegradation of n-Hexadecane in solid-phase of soil by employing immobilized Pseudomonas Aeruginosa on size-optimized coconut fibers.

In this research, biodegradation of hexadecane as a model contaminant in solid soil using both free and immobilized Pseudomonas Aeruginosa, capable of producing biosurfactant, was investigated. Coconut fibers in three mesh sizes were used as a cellulosic biocarrier for immobilization procedure. Bioremediation experiments were monitored for 60 days after incubation at 27 °C in small columns, containing contaminated solid soil, with the capability of aeration from bottom to top. The difference in the number of immobilized bacteria cells on the fibers with different particle sizes, emphasizes the importance of choosing an optimized carrier size. Enhancement in hexadecane degradation up to 50 % at the end of experiments was achieved by immobilized Pseudomonas Aeruginosa on the fibers with a mesh size between 8 and 16 compared to inoculation of free bacteria cells into the soil. Effect of mixing the pretreated fibers with soil and inoculating free cells into this mixture was also investigated compared to free cell experiments without fiber, which led to 28 % decrease in hexadecane degradation. Obtained kinetic equations for experiments confirm the impact of immobilization of bacteria on the enhancement of biodegradation rate and reduction of the half-life of the contaminant is soil.

Metal affinity immobilization of cellulase on Fe3O4 nanoparticles with copper as ligand for biocatalytic applications.

The immobilization of cellulase on amine-functionalized Fe3O4 magnetic nanoparticles (MNPs), via metal affinity immobilization, as a nano-biocatalyst was investigated. Copper was chosen as ligand and loaded onto MNPs in a buffering environment without adding any intermediates. Immobilization conditions were optimized by a 23 full factorial design method. Under optimized working conditions (Cu/MNPs = 1, E/MNPs = 0.11, pH = 6), the relative enzyme activity and the amount of enzyme immobilization were 91% and 164 (mg enzyme/g MNPs), respectively. The immobilized cellulase (tested by carboxymethyl cellulose hydrolysis at 1% concentration) was found to be more stable than the free enzyme. Also, the immobilized enzyme still retained 73% of its initial activity after five cycles of usage. Furthermore, the free and immobilized cellulases retained 70 and 84% of their initial activity after eight days storage at 4 °C, respectively. Immobilization of enzymes, using this method, could be a good and economic option for various industries.

Optimization of cellulase production under solid-state fermentation by a new mutant strain of Trichoderma reesei.

Nowadays, the use of agricultural by-products, as the cheap substrate for the production of value-added products, is of high interest for the researchers and practitioners. Cellulase is a relatively expensive and a very important industrial enzyme where in this study was produced form rice by-products under solid-state fermentation. A new mutant of Trichoderma reesei was used for cellulase production. The effective variables were initially screened by "Plackett and Burman design." Afterward, the main variables including moisture content, P source, incubation temperature, and incubation time were optimized by "one factor at a time design." Finally, the resulting variables including 74% for moisture content, 2 g/L for K2 HPO 4, 30°C of incubation temperature, and 4 days of incubation time were reported as the ultimate optimal condition for cellulase production.

Dynamic mathematical modeling of heat and mass transfer incorporating with the local nutrient and biomass limitation of growth in a packed-bed solid-state bioreactor.

This research develops on our previous semi-mechanistic model that describes the dynamic physical and biochemical processes taking place in a packed-bed bioreactor to analyze the relationship of nutrient limitation, biomass accumulation, metabolic heat generation, and mathematical description of packed-bed porous media. The experimental and simulation data proved that glucose concentration gradients in the biofilm could be neglected due to small biofilm thickness and high diffusivity of glucose in the biofilm. The prediction results also showed that an increase in the initial substrate concentration leads to a rise in the temperature gradient in the bed. The model proposes that if the diameter of substrate particle is too large (r > 0.1 cm), the growth rate will decrease significantly due to the high biomass accumulation in the biofilm, and temperature gradients decrease in the column. This can be used as a strategy to control the overheating problem in the bed.

Statistical factor-screening and optimization in slurry phase bioremediation of 2,4,6-trinitrotoluene contaminated soil.

Since slurry phase bioremediation is a promising treatment for recalcitrant compounds such as 2,4,6-trinitrotoluene (TNT), a statistical study was conducted for the first time to optimize TNT removal (TR) in slurry phase. Fractional factorial design method, 2(IV)(7-3), was firstly adopted and four out of the seven examined factors were screened as effective. Subsequently, central composite design and response surface methodology were employed to model and optimize TR within 15 days. A quadratic model (R(2) = 0.9415) was obtained, by which the optimal values of 6.25 g/L glucose, 4.92 g/L Tween 80, 20.23% (w/v) slurry concentration and 5.75% (v/v) inoculum size were estimated. Validation experiments at optimal factor levels resulted in 95.2% TR, showing a good agreement with model prediction of 96.1%. Additionally, the effect of aeration rate (0-4 vvm) on TR was investigated in a 1-liter bioreactor. Maximum TR of 95% was achieved at 3 vvm within 9 days, while reaching the same removal level in flasks needed 15 days. This reveals that improved oxygen supply in bioreactor significantly reduces bioremediation time in comparison with shake flasks.

Carbon content reduction in a model reluctant clayey soil: slurry phase n-hexadecane bioremediation.

Clayey soils contaminated with organic pollutants are nowadays one of the important environmental issues as they are highly reluctant to conventional bioremediation techniques. In this study, biodegradability of n-hexadecane as a model contaminant in oil polluted clayey soil by an indigenous bacterium was investigated. Maximal bacterial growth was achieved at 8% (v/v) n-hexadecane as sole carbon and energy sources in aqueous phase. The predominant n-hexadecane uptake mechanism was identified to be biosurfactant-mediated using bacterial adhesion to hydrocarbon (BATH) test and surface tension measurements. The effect of n-hexadecane concentration, soil to water ratio, inoculum concentration and pH on total organic carbon (TOC) reduction from kaolin soil in slurry phase was investigated at two levels in shake flasks using full factorial experimental design method where 10,000 (mg n-hexadecane)(kg soil)(-1), soil-water ratio of 1:3, 10% (v/w) inoculum and pH of 7 resulted in the highest TOC reduction of 70% within 20 days. Additionally, slurry bioreactor experiments were performed to study the effect of various aeration rates on n-hexadecane biodegradation during 9 days where 2.5 vvm was found as an appropriate aeration rate leading to 54% TOC reduction. Slurry phase bioremediation is shown to be a successful method for remediation of clayey reluctant soils.

Supercritical fluid disruption of Ralstonia eutropha for poly(beta-hydroxybutyrate) recovery.

A new method for disruption of Gram-negative bacterium Ralstonia eutropha by supercritical CO(2) for poly(beta-hydroxybutyrate) (PHB) recovery is proposed. The effects of different parameters such as exposure time, pressure, temperature, volume of methanol as a modifier, and culture history on cell disruption efficiency were investigated using Taguchi's statistical approach to determine optimum conditions. The optimum conditions for cell disruption and PHB recovery were as follows: exposure time, 100 min; pressure, 200 atm; temperature, 40 degrees C; volume of methanol, 0.2 mL. The cell culture time was less significant. At optimum conditions, the maximum efficiency of PHB recovery was found to be 89%. The proposed method is comparable with other recovery methods in terms of the percentage of PHB recovery, while it is environmentally more benign.