Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters











Publication year range
1.
R. Inst. Adolfo Lutz ; 67(2): 106-112, 2008.
Article in Portuguese | VETINDEX | ID: vti-453037

ABSTRACT

The influence of the plating medium used for the recovery of survivors, on the D-values for L. monocytogenes, in formulations for beef hamburger was investigated: 10% fat (F1), 20% fat (F2), 10% fat and 1.5% sodium chloride (F3) and 10% fat and 10.5% hydrated texturized soy protein (F4) were prepared and inoculated with a mixture of five strains of L. monocytogenes. Using tryptose phosphate agar containing 1% sodium pyruvate (TPAP) D-values (min) ranged from 36.11 (F1) to 62.76 (F3) at 55.0C, from 2.55 (F2) to 4.32 (F3) at 60.0C and from 0.34 (F1 e F2) to 0.52 (F3) at 65.0C. The D-values, using lithium chloride phenylethanol moxalactam medium (LPM) for enumeration, ranged from 23.05 (F1) to 38.54 (F3) at 55.0C; from 1.81 (F2) to 3.06 (F3) at 60.0C and from 0.25 (F2) to 0.37 (F3) at 65.0C. The z-values (C), using the TPAP recovery data, ranged from 4.81 (F3) to 4.95 (F4) and from 4.95 (F3) to 5.21 (F4) using the LPM recovery data. The authors concluded that the heat resistance of L. monocytogenes in formulations for beef hamburger can be underestimated when LPM is used as the recovery medium.


A influência do meio de cultura na recuperação da população sobrevivente sobre os valores D para L. monocytogenes foi avaliada em formulações de hambúrguer bovino: 10% de gordura (F1), 20% de gordura (F2), 10% de gordura e 1,5% de cloreto de sódio (F3) e 10% de gordura e 10,5% de proteína de soja texturizada hidratada (F4), inoculadas com suspensão mista de 5 cepas de L. monocytogenes. Empregando-se ágar triptose fosfato + 1% de piruvato de sódio (ATFP) os valores D (min) variaram de 36,11 (F1) a 62,76 (F3) a 55,0C, de 2,55 (F2) a 4,32 (F3) a 60,0C e de 0,34 (F1 e F2) a 0,52 (F3) a 65,0C. Os valores D calculados utilizando-se ágar cloreto de lítio feniletanol moxalactam (LFM) variaram de 23,05 (F1) a 38,54 (F3) a 55,0C; 1,81 (F2) a 3,06 (F3) a 60,0C e de 0,25 (F2) a 0,37 (F3) a 65,0C. Os valores z (C) variaram de 4,81 (F3) a 4,95 (F4) com ATFP e 5,08 (F2) a 5,21 (F4) com LFM. Os autores concluíram que a resistência térmica de L. monocytogenes em formulações para hambúrguer bovino foi subestimada até 1,6-1,7 vezes quando o meio LFM foi utilizado.

SELECTION OF CITATIONS
SEARCH DETAIL