ABSTRACT
To investigate the effect of a constant direct electrical current on the mineral deposition in an experimental osseous defect, 8 microamperes of current were applied across a circular osteotomy placed in the lateral cortex of an avian metatarsus. Mineral deposition was measured to quantitate bone formation in vivo using iodine-125 absorptiometry. The electrical current had no effect either on the initiation or the rate of mineral deposition. However, the quantity of mineralized callus was significantly greater in the treated than untreated bones.
Subject(s)
Electric Stimulation , Fractures, Bone/physiopathology , Metatarsus/injuries , Wound Healing , Animals , Bony Callus/analysis , Chickens , Male , Minerals/analysis , OsteotomyABSTRACT
An in vivo, quantitative, non-destructive method of measuring bone mineral deposition in a repairing osseous defect has been developed. A circular osteotomy, used as a controlled fracture, was found to be extremely reproducible, and could be introduced with a minimum of trauma. The rate and magnitude of mineralization of the osteotomy was directly related to the attenuation of a highly collimated photon beam produced by Iodine-125. Changes in bone mineral content of 4% can be detected using this system.
Subject(s)
Fractures, Bone , Animals , Calcification, Physiologic , Chickens , Iodine Radioisotopes , Methods , Wound HealingABSTRACT
Adult roosters with experimental circular osteotomy defects in the metatarsus were placed on chronic heparin therapy. In vivo measurement of bone mineral content changes of the osteotomy using Iodine-125 show no adverse affect of heparin treatment on remineralization. However, the weight of dry-defatted femurs was decreased and suggests that there was significant heparin-induced systemic bone demineralization.
Subject(s)
Bone Regeneration/drug effects , Heparin/pharmacology , Animals , Bone and Bones/analysis , Chickens , Male , Minerals/analysis , Osteolysis, Essential/chemically induced , OsteotomyABSTRACT
The effect of diethylstilbestrol on healing experimental osseous defects in adult roosters was studied by means of I-125 photon absorptiometry. Although the two dose levels utilized in this study both resulted in elevated plasma calcium and lipid levels and significantly increased the mineral and organic fractions of femoral bone, diethylstilbestrol treatment over a six-week period had no effect on the rate or magnitude of mineralization in the repairing osseous defect.
