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1.
J Egypt Public Health Assoc ; 73(5-6): 501-18, 1998.
Article in English | MEDLINE | ID: mdl-17217021

ABSTRACT

Recent researches are concerned with topics of newborn care giving, maternal knowledge and practices because it facilitates growth and development, protection against infection and detection of any abnormalities. The purpose of this study was to assess the mothers' knowledge and practices of basic newborn care given at home. The total sample included 55 primipara and multipara mothers with newborn babies. They were selected from Obstetric University Hospital in Tanta City. Interview questionnaire and observation checklists were designed to fulfill the aim of the study. The study revealed that mothers' knowledge and practices were within good and satisfactory average scores in most of the studied items related to newborn care giving at home except breast feeding. found between Significant differences were primipara and multipara mothers for most of the studied topics related to different topics of newborn care giving. practices were within good and satisfactory average scores in most of the studied items related to newborn care giving at home except breast feeding. Significant differences were found between primipara and multipara mothers for most of the studied topics related to different topics of newborn care giving.


Subject(s)
Attitude to Health , Health Knowledge, Attitudes, Practice , Infant Care , Mothers/education , Parity , Adult , Baths , Breast Feeding , Clothing , Delivery, Obstetric , Educational Measurement , Egypt , Female , Health Education , Health Services Needs and Demand , Hospitals, Maternity , Hospitals, University , Hospitals, Urban , Humans , Hygiene , Infant Care/methods , Infant Care/psychology , Infant, Newborn , Mothers/psychology , Mothers/statistics & numerical data , Occupations , Pregnancy , Surveys and Questionnaires
3.
J Egypt Public Health Assoc ; 70(5-6): 523-40, 1995.
Article in English | MEDLINE | ID: mdl-17214173

ABSTRACT

This study examined the relationship between the knowledge of nurses working in Tanta Fever Hospital (N = 54) and their compliance to universal precautions as a prevention of HBV. An interview questionnaire and observation checklist were designed to fulfill the aim of the study. The study revealed that nurses' knowledge was below the average score in half of the items studied, while their performance was below the average in all the items except for care of contaminated instruments. Although no significant relation was found between nurses' knowledge and their performance regarding the universal precautions, nurses having less than 5 years of experience had a higher score of performance than those having more than 5 years of experience in 6 items. The study reflects the need for in-service educational and training programs on infection control for nurses.


Subject(s)
Cross Infection/prevention & control , Guideline Adherence/standards , Hepatitis B/prevention & control , Nursing Staff, Hospital/education , Practice Guidelines as Topic , Universal Precautions , Attitude of Health Personnel , Clinical Competence/standards , Cross Infection/transmission , Education, Nursing, Continuing , Educational Measurement , Egypt , Equipment Contamination/prevention & control , Health Knowledge, Attitudes, Practice , Health Services Needs and Demand , Hepatitis B/transmission , Humans , Infection Control/methods , Infection Control/standards , Inservice Training , Medical Waste Disposal/standards , Nursing Audit , Nursing Education Research , Nursing Evaluation Research , Nursing Methodology Research , Nursing Staff, Hospital/psychology , Surveys and Questionnaires
4.
J Chromatogr ; 525(2): 339-47, 1990 Feb 23.
Article in English | MEDLINE | ID: mdl-1691743

ABSTRACT

A silica gel thin-layer chromatographic procedure is described for the study of the myelin lipid patterns in a small phylogenetic series of nerve tissue specimens. It involves the selective staining by the thiazine dye thionine and the interpretations were facilitated by a preceding primary alkylamine O-deacylation step. Glycolipids, including sulfatides, and ethanolamine plasmalogens were the principal characterizing lipids.


Subject(s)
Chromatography, Thin Layer , Lipids/analysis , Myelin Sheath/analysis , Phenothiazines , Phylogeny , Acylation , Animals , Ceramides/analysis , Decapodiformes/analysis , Ethylamines , Fishes , Glycolipids/analysis , Guinea Pigs , Lampreys , Methylamines , Necturus , Sharks , Staining and Labeling
6.
Comp Biochem Physiol B ; 89(1): 111-8, 1988.
Article in English | MEDLINE | ID: mdl-3356123

ABSTRACT

1. The plasmalogen profiles of a series of hearts from fish to mammals were obtained by various TLC analyses. 2. All specimens (ventricular) contained ethanolamine plasmalogen and some choline plasmalogen, as well. 3. The distribution of these two plasmalogen species was relatable, in part, to (a) phylogeny and (b) ontogeny. 4. There were exceptions. 5. The appearance of choline plasmalogen was preceded by its alkylacyl precursor, suggesting plasmalogenation by a base-specific delta 1-alkyl desaturase. 6. From the data, we have raised some questions as to the metabolic role played by the plasmalogens and precursors as occupants of myocardial mitchondrial membranes.


Subject(s)
Myocardium/metabolism , Phosphatidylcholines/metabolism , Plasmalogens/metabolism , Vertebrates/growth & development , Animals , Chromatography, Thin Layer , Reference Values , Species Specificity , Vertebrates/metabolism
7.
Comp Biochem Physiol B ; 86(1): 83-7, 1987.
Article in English | MEDLINE | ID: mdl-3829635

ABSTRACT

With the aid of a variety of TLC preparations the phospholipid and neutral lipid profiles of guinea-pig pancreas and small intestine has been established. Changes in these profiles, subsequent to incubation of these tissues and prior to lipid extraction, has provided evidence relating to their respective lipolytic enzyme capabilities, mainly in the phospholipase group.


Subject(s)
Intestine, Small/metabolism , Lipid Metabolism , Pancreas/metabolism , Animals , Female , Guinea Pigs , Lipolysis , Lysophospholipase/metabolism , Male , Phospholipases A/metabolism
8.
J Chromatogr ; 374(1): 61-72, 1986 Jan 10.
Article in English | MEDLINE | ID: mdl-3949936

ABSTRACT

We have isolated a minor phosphatidyl ethanolamine component from pancreas and a minor phosphatidyl choline component from retina, which were revealed by their separate thin-layer chromatographic properties on silica gel and aluminum oxide sheets, respectively. We have described in some detail a number of modifications in thin-layer chromatography methodology which enhances the opportunity for assessing the glycerophospholipid and neutral lipid composition of tissues, as attested by a diverse set of examples, and have pointed out some of the associated technical problems.


Subject(s)
Lipids/analysis , Phospholipids/analysis , Aluminum Oxide , Animals , Cats , Cattle , Chromatography, Thin Layer , Densitometry , Eye/analysis , Myocardium/analysis , Pancreas/analysis , Plasmalogens/analysis , Rabbits , Seeds/analysis , Species Specificity , Swine , Turtles
9.
Arch Androl ; 11(1): 45-51, 1983 Aug.
Article in English | MEDLINE | ID: mdl-6414393

ABSTRACT

Seventy-five diabetic male and female children and 75 matched controls were classified according to pubertal staging. Blood samples were assayed for gonadotropins and gonadal hormones. The ultimate adult height in diabetic patients was 5 cm less than that of controls. Almost all diabetic children had a retarded bone age. Levels of serum gonadotropins and gonadal hormones did not differ markedly between diabetic and healthy children. The delay in growth and maturation was not due to hormonal failure, but probably to chronic undernutrition of body cells and failure to utilize the amino acids for protein anabolism related to relative insulin deficiency.


Subject(s)
Diabetes Mellitus, Type 1/physiopathology , Ovary/physiopathology , Pituitary Gland/physiopathology , Testis/physiopathology , Adolescent , Adult , Child , Diabetes Mellitus, Type 1/complications , Female , Follicle Stimulating Hormone/blood , Humans , Luteinizing Hormone/blood , Male , Progesterone/blood , Puberty, Delayed/etiology , Sex Factors , Testosterone/blood
10.
Comp Biochem Physiol B ; 76(3): 399-407, 1983.
Article in English | MEDLINE | ID: mdl-6357624

ABSTRACT

An overview of the melanins and lipofuscin is presented, taking an integrating account of their morphological relationships and chemical characteristics wherever possible. This has required inclusion of the ommochromes, the schlerotizing process, and consideration of related neurotransmitters. A number of questions are raised and commented upon.


Subject(s)
Lipofuscin/metabolism , Melanins/metabolism , Pigments, Biological/metabolism , Animals , Humans , Kidney/physiology , Lipofuscin/analysis , Liver/physiology , Melanins/analysis , Ocular Physiological Phenomena , Phenylalanine/metabolism , Skin Physiological Phenomena , Species Specificity
12.
Comp Biochem Physiol B ; 73(4): 873-9, 1982.
Article in English | MEDLINE | ID: mdl-7151425

ABSTRACT

1. We have re-examined the lipids from myocardial infarcts of cat, dog, rabbit and man, mainly through TLC methods, and confirm the identity of cat and dog "infarct plasmalogen" as an N-acyl phosphatidyl ethanolamine (NAPE). This substance was not detected in infarcts of rabbit and man. 2. We have extended our observations on a similar phosphatide, as the plasmalogen form, naturally occurring in the brain and optic nerve of fish. 3. Supporting evidence for the NAPE identity was provided from non-plasmalogen forms isolated from peas and lentils. 4. NAPE in all of its forms was shown to be a reluctant substrate for the phospholipase A2 of snake venoms. 5. Co-chromatography problems involving NAPE, semi-lyso cardiolipin and bis-phosphatidic acid are documented and their relationship to the infarct phenomenon discussed.


Subject(s)
Myocardial Infarction/metabolism , Phosphatidic Acids/analysis , Phosphatidylethanolamines/analysis , Plasmalogens/analysis , Animals , Brain Chemistry , Cats , Chromatography, Thin Layer , Densitometry , Dogs , Myocardium/analysis , Optic Nerve/analysis , Rabbits , Species Specificity
13.
Comp Biochem Physiol B ; 71(1): 101-4, 1982.
Article in English | MEDLINE | ID: mdl-7060351

ABSTRACT

1. The phosphoglyceride and sphingolipid content of dog pancreas has been determined and their in vitro response to the endogenous lipolytic enzymes, initiated by trypsin, at pH 7.4 was examined by TLC technology. 2. The glyceryl ether phosphatides were found to consist primarily of PE and PC, each with alkyl and alk-1-enyl components both of which were hydrolyzed to their respective 1-radyl lyso derivatives under the described incubation conditions. 3. A new TLC visualizing reaction for lipids, OSPAS, is described.


Subject(s)
Lipid Metabolism , Pancreas/metabolism , Phospholipases A/metabolism , Phospholipases/metabolism , Trypsin/metabolism , Animals , Dogs , Female , Hydrogen-Ion Concentration , Hydrolysis , Lysophosphatidylcholines/metabolism , Male , Pancreas/enzymology , Phosphatidylcholines/metabolism , Phosphatidylethanolamines/metabolism , Phospholipases A2
19.
J Chromatogr ; 107(1): 155-66, 1975 Apr 09.
Article in English | MEDLINE | ID: mdl-237934

ABSTRACT

A method of analysis is described which permits the facile evaluation of the neutral glyceryl ether lipids (mild alkaline hydrolysis) and total glyceryl ether lipid (Vitride reduction) thereby allowing an assessment of the phosphatide contribution. The alkyl and alk-enyl glyceryl ethers are chromatographically resolvable on Gelman glass-fiber type SG and Whatman SG-81 and detected by the periodic acid-Schiff and plasmal reaction, respectively. The attending problems and interpretation are discussed with examples from a number of unicellular organisms and animal tissues.


Subject(s)
Chromatography , Ethers/analysis , Phosphatidylglycerols/analysis , Phospholipids/analysis , Amphibians , Animals , Cats , Female , Fishes , Glass , Humans , Hydrogen-Ion Concentration , Hydrolysis , Liver/analysis , Male , Methods , Myocardium/analysis , Pregnancy , Rats , Reptiles , Solvents , Species Specificity , Tissue Extracts/analysis
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