Study of the role of tumor necrosis factor-α (-308 G/A) and interleukin-10 (-1082 G/A) polymorphisms as potential risk factors to acute kidney injury in patients with severe sepsis using high-resolution melting curve analysis.

RATIONAL: Septic acute kidney injury (AKI) is a prevalent complication in intensive care units with an increased incidence of complications. OBJECTIVE: The aim of the present study was to assess the use of high-resolution melting curve (HRM) analysis in investigating whether the genetic polymorphisms; -308 G/A of tumor necrosis factor-α (TNF-α), and -1082 G /A of Interleukin-10 (IL-10) genes may predispose patients diagnosed with severe sepsis to the development of AKI. METHODS: One hundred and fifty patients with severe sepsis participated in the present study; only sixty-six developed AKI. Both polymorphisms were studied using HRM analysis. MAIN FINDINGS: The low producer genotype of both studied polymorphism of TNF-α and IL-10 genes was associated with AKI. Using logistic regression analysis, the low producer genotypes remained an independent risk factor for AKI. A statistically significant difference was detected between both studied groups as regards the low producer genotype in both TNF-α (-308 G/A) and interleukin-10 (IL-10) (-1082 G/A) polymorphisms being prevalent in patients developing AKI. Principle conclusions: The low producer genotypes of both TNF-α (-308 G/A) and IL-10 (-1082 G/A) polymorphisms could be considered a risk factor for the development of AKI in critically ill patients with severe sepsis, thus management technique implemented for this category should be modulated rescuing this sector of patients from the grave deterioration to acute kidney injury. Using HRM for genotyping proved to be a highly efficient, simple, cost-effective genotyping technique that is most appropriate for the routine study of large-scale samples.

Usefulness of mitral annular plane systolic excursion in assessment of left ventricular systolic function in mechanically ventilated obese patients.

BACKGROUND: The management of mechanically ventilated patients depends on a combination of clinical skills and measurement of physiological parameters. OBJECTIVES: We evaluated the role of mitral annular plane systolic excursion (MAPSE) in the determination of left ventricular systolic function (LVSF) in mechanically ventilated obese patients. METHODS: A convenient sample of 60 of 68 obese, mechanically ventilated patients in the intensive care unit were included in the study. Transthoracic echocardiogram MAPSE measurement (averaged from 3 consecutive cycles) on 2 separate days, vs the eyeball ejection fraction (classic clinical method) on 2 separate days, and vs the biplane Simpson (classic research and consultative echocardiogram method) on 2 separate days was obtained. In patients with non sinus rhythm, measurements were collected over 5 to 10 heartbeats. RESULTS: There was a significant positive correlation between MAPSE and LVSF assessment using the eyeball method and Simpson method (r= 0.89 and 0.87, respectively; P< .001). Mitral annular plane systolic excursion was significantly shorter in duration (P< .001). CONCLUSIONS: Mitral annular plane systolic excursion is useful, an easier technique, and shorter in duration compared with the eyeball method in the determination of LVSF in mechanically ventilated obese patients.