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J Sci Food Agric ; 92(4): 764-71, 2012 Mar 15.
Article in English | MEDLINE | ID: mdl-21953440

ABSTRACT

BACKGROUND: Chapati preparation involves various processing steps such as mixing the flour into dough, sheeting and baking. During these processing steps, flour components are likely to undergo changes in their nutrient and polyphenol composition and their antioxidant properties due to phenol-mediated crosslinking of proteins and carbohydrates. Therefore, in the present study, changes in nutritional, nutraceutical and antioxidant properties of chapatis prepared from doughs treated with amylases and xylanase were determined. RESULTS: An increase in insoluble dietary fibre content and a decrease in soluble polyphenol content were observed during preparation of control chapatis from whole wheat flours. However, significant increases in soluble dietary fibre and soluble polyphenol contents were observed in chapatis prepared from amylase-treated doughs compared with control chapatis. Extracts of chapatis prepared from amylase- and xylanase-treated doughs showed better antioxidant properties than extracts of control chapatis. Among these enzyme treatments, chapatis prepared from amylase-treated doughs showed better antioxidant properties than chapatis prepared from xylanase-treated doughs. High-performance liquid chromatography analysis of extracts of chapatis prepared from doughs treated with amylases showed the presence of potential antioxidant phenolic acids such as caffeic, gentisic and syringic acids in addition to the phenolic acids present in control chapatis. CONCLUSION: Treatment of doughs with amylases increased the contents of soluble dietary fibre and soluble polyphenols as well as improving the antioxidant properties of chapatis.


Subject(s)
Antioxidants/analysis , Bread/analysis , Dietary Fiber/analysis , Endo-1,4-beta Xylanases/metabolism , Flour/analysis , Polyphenols/analysis , alpha-Amylases/metabolism , Antioxidants/chemistry , Antioxidants/isolation & purification , Antioxidants/metabolism , Aspergillus oryzae/enzymology , Bacillus subtilis/enzymology , Bacterial Proteins/metabolism , Caffeic Acids/analysis , Caffeic Acids/chemistry , Caffeic Acids/isolation & purification , Chromatography, High Pressure Liquid , Diet/ethnology , Dietary Fiber/metabolism , Food Handling , Fungal Proteins/metabolism , Gallic Acid/analogs & derivatives , Gallic Acid/analysis , Gallic Acid/chemistry , Gallic Acid/isolation & purification , Gentisates/analysis , Gentisates/chemistry , Gentisates/isolation & purification , India , Polyphenols/chemistry , Polyphenols/isolation & purification , Polyphenols/metabolism , Quality Control , Solubility
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