ABSTRACT
In recent years, oxidative stress and free radicals have been implicated in impaired wound healing. Grape (Vitis vinifera) seed extract (GSE) possesses anti-inflammatory and antioxidant properties. The present study was undertaken to assess the potential activity of grape seed hydroalcoholic extract in wound healing in rabbits. Rabbits of either sex were subjected to a 20 × 20 mm square excision made over the skin of the back. The animals were randomly divided into seven experimental groups, as negative and positive control, eucerin and treatments. Negative control group did not receive any treatment. Positive control and eucerin groups received phenytoin cream (1%) and topical eucerin, respectively, twice a day from the beginning of experiments to complete wound closure. Treatment groups were treated topically by cream of GSE (2, 5, 10 and 70% w/w) in eucerin base, twice daily. For evaluation of the percentage of wound healing, area of the wound was measured daily. Histological studies were performed on the 7th and 15th days of treatments. After complete healing, hydroxyproline content and tensile strength measurement of tissue samples were done. Results showed that there were statistically significant differences between GSE treatments groups and eucerin animals (P < 0·05) in most of the days. Rabbits treated with 2% GSE had best results (completed healing in 13 days, higher hydroxyproline content and higher tissue resistance). We concluded that the extract of 2% GSE administered topically has a good potential to promote wound healing in wound model of rabbits.
Subject(s)
Granulation Tissue/drug effects , Grape Seed Extract/administration & dosage , Wound Healing/drug effects , Wounds and Injuries/drug therapy , Administration, Topical , Animals , Disease Models, Animal , Female , Follow-Up Studies , Granulation Tissue/pathology , Male , Rabbits , Treatment Outcome , Vitis , Wounds and Injuries/pathologyABSTRACT
Due to the production of reactive oxygen species (ROS), oxidative stress has been implicated in the pathogenesis of silica-induced lung fibrosis. So it is hypothesized that grape seed extract (GSE) or vitamin E (Vit E) as antioxidants may ameliorate some symptoms of the disease. Male Wistar albino rats were divided into 7 groups: rats in group I instilled intratracheally (IT) with a single dose of silica suspension (50mg/rat) as positive control (PC). Treatment groups (II-IV) received Vit E (20 IU/kg/day), GSE (150 mg/kg/day), or Vit E+GSE simultaneously orally 1 day after instillation of silica. Groups V and VI were given oral GSE or Vit E after instillation of the equivalent volume of saline (IT) as controls for GSE or Vit E. Rats of group VII only instilled saline (IT) as negative control. After 90 days animals were sacrificed and plasma-malondialdehyde (p-MDA) and lung tissue hydroxyproline (HP) were quantified. The lungs were also investigated for histopathological changes. The mean concentrations of p-MDA and HP in studied groups (I-VII) were 1.95, 2.77, 0.72, 0.81, 0.64, 0.94, 1.02 micromolMDA/L(plasma) and 28.476, 27.85, 22.83, 22.64, 15.40, 18.31, 18.51 mgHP/g(tissue), respectively. Silica caused a significant increase in HP content of lungs and MDA levels in the plasma except in GSE-treated groups (III and IV). According to the results of this study GSE could reduce the fibrogenic effect of silica. However; no synergistic effect was observed after co-administration of GSE and Vit E.
