Manipulating redox conditions to enhance in situ bioremediation of RDX in groundwater at a contaminated site.

Surficial application of waste glycerol (WG) for enhanced bioremediation was tested in situ at an old military range site to address hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) contaminated groundwater. This treatment was effective in inducing strong reducing conditions (range: -4 to -205 mV) and increasing the concentrations of organic carbon (from 10 to 729 mg/L) and fatty acids (from 0 to 940 mg/L) concomitantly with a decrease in RDX concentrations (range: 17 to 143 µg/L) to below detection limits (0.1 µg/L) in 2 of the 3 monitoring wells (MWs) evaluated. None of these changes were observed in the control MW. RDX disappeared without the detection of any common anaerobic nitroso degradation intermediates, with the exception of one MW where the concentration of organics did not significantly increase (range: 10 to 20 mg/L), suggesting the conditions were not favourable for biodegradation. Ecotoxicological analysis suggested that the use of WG may have some dose-related deleterious effects on different soil and aquatic receptors. Analysis of the microbial community composition, using 16S rRNA gene amplicon sequences, which provided insight into whether the process design had selected for and stimulated the optimal microbial populations, indicated co-existence of numerous Operational Taxonomic Units (OTUs) belonging to groups known to be capable of RDX degradation under anaerobic conditions, with a positive link between Geobacter spp. enrichment and the presence of RDX nitroso metabolites. Overall, the results from this field test show that this treatment process can provide an effective long-term, semi-passive remediation option for RDX contaminated groundwater.

In situ pilot test for bioremediation of energetic compound-contaminated soil at a former military demolition range site.

Bioremediation was performed in situ at a former military range site to assess the performance of native bacteria in degrading hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and 2,4-dinitrotoluene (2,4-DNT). The fate of these pollutants in soil and soil pore water was investigated as influenced by waste glycerol amendment to the soil. Following waste glycerol application, there was an accumulation of organic carbon that promoted microbial activity, converting organic carbon into acetate and propionate, which are intermediate compounds in anaerobic processes. This augmentation of anaerobic activity strongly correlated to a noticeable reduction in RDX concentrations in the amended soil. Changes in concentrations of RDX in pore water were similar to those observed in the soil suggesting that RDX leaching from the soil matrix, and treatment with waste glycerol, contributed to the enhanced removal of RDX from the water and soil. This was not the case with 2,4-DNT, which was neither found in pore water nor affected by the waste glycerol treatment. Results from saturated conditions and Synthetic Precipitation Leaching Procedure testing, to investigate the environmental fate of 2,4-DNT, indicated that 2,4-DNT found on site was relatively inert and was likely to remain in its current state on the site.