ABSTRACT
The micronemal protein MIC3 of Toxoplasma gondii plays a predominant role in the early phase of the invasion process. In this research, the expression and location of protein MIC3 and fusion protein EGFP-MIC3 were observed in host cells and in T. gondii respectively. Protective experiments of DNA vaccine pcDNA3-MIC3 (pMIC3i) in animals showed that the vaccine could significantly prolong the survival time of the mice challenged by virulent RH strains of T. gondii. All mice vaccinated with plasmid pcDNA3-MIC3 have been elicited specific humoral immunity and cellular immunity. The cellular immune response was associated with the increase of the CD4(+) and CD8(+) T lymphocytes and the decrease of the CD4(+)/CD8(+) T lymphocyte ratio evidently. It indicated that the mic3 DNA vaccine could stimulate the host resisting Toxoplasma mainly by the way of CD8(+)CTL cells. In conclusion, a potent DNA vaccine pcDNA3-MIC3 could elicit a strong specific immune response and induce effective protection against T. gondii challenge in Kunming mice, suggesting that mic3 is a potential vaccine candidate against toxoplasmosis.
Subject(s)
Protozoan Proteins/immunology , Protozoan Vaccines/immunology , Toxoplasma/immunology , Toxoplasmosis/prevention & control , Vaccines, DNA/immunology , Animals , COS Cells , Cell Line , Chlorocebus aethiops , Female , Gene Expression Regulation , HeLa Cells , Humans , Immunity, Cellular , Immunity, Humoral , Mice , Recombinant Proteins/immunology , Survival Analysis , Time Factors , Toxoplasmosis/immunology , Toxoplasmosis/parasitologyABSTRACT
OBJECTIVE@#To investigate the expressions of Survivin protein and nm23 protein and the relationship among the expressions and axillary lymph node metastasis in breast cancer.@*METHODS@#The expression of Survivin and nm23 in 80 cases of breast cancer tissues were detected by immunohistochemistry SP method, and their correlation with axillary lymph node metastasis and 5-year disease free survival (DFS) were analysed.@*RESULTS@#Survivin protein positive expression rate was 68.75% (55/80) in breast cancer tissues, which had positive correlation with the axillary lymph nodes metastasis but negative correlation with 5 years FS (P 0.05).@*CONCLUSION@#The anti-apoptosis effect of Survivin protein and the anti-metastasis effect of nm23 protein may be important in the occurrence and advancement of breast cancer, suggesting that it may be a new indicator of prognostic and judgement in breast cancer.
