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1.
Hum Genet ; 141(5): 1017-1026, 2022 May.
Article in English | MEDLINE | ID: mdl-34426855

ABSTRACT

Due to a number of recent achievements, the field of prenatal medicine is now on the verge of a profound transformation into prenatal genomic medicine. This transformation is expected to not only substantially expand the spectrum of prenatal diagnostic and screening possibilities, but finally also to advance fetal care and the prenatal management of certain fetal diseases and malformations. It will come along with new and profound challenges for the normative framework and clinical care pathways in prenatal (and reproductive) medicine. To adequately address the potential ethically challenging aspects without discarding the obvious benefits, several agents are required to engage in different debates. The permissibility of the sequencing of the whole fetal exome or genome will have to be examined from a philosophical and legal point of view, in particular with regard to conflicts with potential rights of future children. A second requirement is a societal debate on the question of priority setting and justice in relation to prenatal genomic testing. Third, a professional-ethical debate and positioning on the goal of prenatal genomic testing and a consequential re-structuring of clinical care pathways seems to be important. In all these efforts, it might be helpful to envisage the unborn rather not as a fetus, not as a separate moral subject and a second "patient", but in its unique physical connection with the pregnant woman, and to accept the moral quandaries implicitly given in this situation.


Subject(s)
Genetic Testing , Prenatal Diagnosis , Child , Female , Fetus , Genomics , Humans , Pregnancy , Pregnant Women
2.
J Pediatr ; 232: 214-219, 2021 05.
Article in English | MEDLINE | ID: mdl-33450221

ABSTRACT

OBJECTIVE: To examine the general cognitive and psychosocial development in children and adolescents having a co-twin with Down syndrome. STUDY DESIGN: A case control study with an individually matched control group was conducted. Participants included families with twins discordant for Down syndrome as well as with typically developing twins. The group of unaffected co-twins aged 4-16 years was compared with a control group of typically developing twins in terms of general cognitive abilities, behavioral problems, and prosocial behavior. The age and sex and the sex composition of the twins were individually matched. The Sijnders-Oomen nonverbal intelligence test was applied to assess children's IQ, and parents completed the Strength and Difficulties Questionnaire. RESULTS: The unaffected co-twins did not differ from typically developing twins with respect to their IQ. Concerning the psychosocial development, significantly heightened values in unaffected co-twins twins were only obtained for the conduct problems scale (P = .01; r = 0.45), neither for the total difficulties score nor for the other behavioral problem scales significant differences were found. CONCLUSIONS: The general cognitive development of the unaffected co-twin of children with Down syndrome is not affected by the presence of their Down syndrome twin. Unaffected co-twins showed increased conduct problems, which is most pronounced in the younger children.


Subject(s)
Adolescent Development , Altruism , Child Development , Cognition , Diseases in Twins , Down Syndrome , Problem Behavior , Adolescent , Case-Control Studies , Child , Child, Preschool , Female , Humans , Intelligence , Male , Psychological Tests , Twins/psychology
5.
Am J Med Genet A ; 176(11): 2284-2291, 2018 11.
Article in English | MEDLINE | ID: mdl-30070765

ABSTRACT

Triple-X syndrome is a common sex chromosome aneuploidy, which appears in 1 out of 1,000 females. The aim of our study was to describe the behavioral features of a large group of girls and women with triple-X in comparison to a control group. A total of 72 subjects with triple-X and 69 subjects of an age-matched control group were included. Psychological and behavioral questionnaires were allocated to three age groups, representing a range of ages from young childhood to adulthood. Regarding the females between 4 and 7 years of age, we found significant differences for social problems, attention problems, and school performance. For the age group 8-17 years, we found larger significant differences for the majority of the scales listed in the child behavior checklist. The most significant differences (p < .001) were from total behavior problems, internalizing problems, and four other scales. Young females with triple-X have significantly lower general self-esteem, especially concerning school and family. In the adults, there were significant differences concerning psychological symptoms and distress, with higher scores in the triple-X subjects. Regardless, their mean scores were still in the normal range. We did not find clinical evidence for more than 50% of the triple-X females in any age group, indicating that approximately half of them do not have behavioral problems, and that more than 60% do not differ in their competence from the control group. However, our findings suggest that triple-X influences mental health and the overall well-being of the individuals across their whole life spans.


Subject(s)
Behavior , Sex Chromosome Disorders of Sex Development/psychology , Adolescent , Adult , Case-Control Studies , Checklist , Child , Child Behavior , Child, Preschool , Chromosomes, Human, X , Female , Humans , Self Concept , Sex Chromosome Aberrations , Trisomy , Young Adult
6.
PLoS One ; 10(4): e0124112, 2015.
Article in English | MEDLINE | ID: mdl-25901601

ABSTRACT

BACKGROUND: In asymptomatic Marfan syndrome (MFS) patients we evaluated the relationship between the types of fibrillin-1 (FBN1) gene mutation and possible altered left ventricular (LV) function as assessed by three-dimensional speckle tracking echocardiography (3D-STE). METHODS AND RESULTS: Forty-five MFS patients (mean age 24 ± 15 years) and 40 age-matched healthy controls were studied. Genetic evaluation for the FBN1 gene was carried on 32 MFS patients. Gene mutation (n = 15, 47%) was classified as mild when the mutation resulted in nearly normally functioning protein, while mutations resulting in abnormally function protein were considered to be severe (n = 17, 53%). All patients and controls underwent 3D-STE for evaluation of LV function by an echocardiographer blinded to the results of the genetic testing. Compared to controls, MFS patients had significantly lower 3D-STE derived LV ejection fraction (EF, 57.43 ± 7.51 vs. 62.69 ± 4.76%, p = 0.0001), global LV longitudinal strain (LS, 14.85 ± 2.89 vs. 17.90 ± 2.01%, p = 0.0001), global LV circumferential strain (CS, 13.93 ± 2.81 vs. 16.82 ± 2.17%, p = 0.0001) and global LV area strain (AS, 25.76 ± 4.43 vs. 30.51 ± 2.61%, p = 0.0001). Apart from the global LV LS all these parameters were significantly lower in patients with severe gene mutation than in those with mild mutation (p < 0.05). In the multivariate linear regression analysis only the type of mutation had a significant influence on the 3D-STE derived LVEF (p = 0.017), global CS (p = 0.005) and global AS (p = 0.03). CONCLUSIONS: In asymptomatic MFS patients latent LV dysfunction can be detected using 3D STE. The LV dysfunction is mainly related to the severity of gene mutation, suggesting possible primary cardiomyopathy in MFS patients.


Subject(s)
Cardiomyopathies/genetics , Marfan Syndrome/genetics , Microfilament Proteins/genetics , Mutation , Ventricular Dysfunction, Left/genetics , Adolescent , Adult , Asymptomatic Diseases , Cardiomyopathies/diagnostic imaging , Cardiomyopathies/pathology , Case-Control Studies , Child , Echocardiography, Three-Dimensional , Female , Fibrillin-1 , Fibrillins , Gene Expression , Humans , Male , Marfan Syndrome/diagnostic imaging , Marfan Syndrome/pathology , Microfilament Proteins/metabolism , Middle Aged , Regression Analysis , Stroke Volume , Ventricular Dysfunction, Left/diagnostic imaging , Ventricular Dysfunction, Left/pathology
7.
Oncotarget ; 6(8): 5918-31, 2015 Mar 20.
Article in English | MEDLINE | ID: mdl-25537509

ABSTRACT

Glioblastoma multiforme (GBM) is the most aggressive and malignant subtype of human brain tumors. While a family clustering of GBM has long been acknowledged, relevant hereditary factors still remained elusive. Exome sequencing of families offers the option to discover respective genetic factors.We sequenced blood samples of one of the rare affected families: while both parents were healthy, both children were diagnosed with GBM. We report 85 homozygous non-synonymous single nucleotide variations (SNVs) in both siblings that were heterozygous in the parents. Beyond known key players for GBM such as ERBB2, PMS2, or CHI3L1, we identified over 50 genes that have not been associated to GBM so far. We also discovered three accumulative effects potentially adding to the tumorigenesis in the siblings: a clustering of multiple variants in single genes (e.g., PTPRB, CROCC), the aggregation of affected genes on specific molecular pathways (e.g., Focal adhesion or ECM receptor interaction) and genomic proximity (e.g., chr22.q12.2, chr1.p36.33). We found a striking accumulation of SNVs in specific genes for the daughter, who developed not only a GBM at the age of 12 years but was subsequently diagnosed with a pilocytic astrocytoma, a common acute lymphatic leukemia and a diffuse pontine glioma.The reported variants underline the relevance of genetic predisposition and cancer development in this family and demonstrate that GBM has a complex and heterogeneous genetic background. Sequencing of other affected families will help to further narrow down the driving genetic causes for this disease.


Subject(s)
Brain Neoplasms/genetics , Exome , Glioblastoma/genetics , Aged , Amino Acid Sequence , Brain Neoplasms/blood , Brain Neoplasms/pathology , Cell Transformation, Neoplastic/genetics , Child , DNA, Neoplasm/blood , DNA, Neoplasm/genetics , Female , Genetic Predisposition to Disease , Glioblastoma/blood , Glioblastoma/pathology , Humans , Male , Middle Aged , Molecular Sequence Data , Pedigree
8.
Mol Genet Genomic Med ; 2(2): 176-85, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24689081

ABSTRACT

Cerebral cavernous malformations (CCM) are prevalent vascular malformations occurring in familial autosomal dominantly inherited or isolated forms. Once CCM are diagnosed by magnetic resonance imaging, the indication for genetic testing requires either a positive family history of cavernous lesions or clinical symptoms such as chronic headaches, epilepsy, neurological deficits, and hemorrhagic stroke or the occurrence of multiple lesions in an isolated case. Following these inclusion criteria, the mutation detection rates in a consecutive series of 105 probands were 87% for familial and 57% for isolated cases. Thirty-one novel mutations were identified with a slight shift towards proportionally more CCM3 mutations carriers than previously published (CCM1: 60%, CCM2: 18%, CCM3: 22%). In-frame deletions and exonic missense variants requiring functional analyses to establish their pathogenicity were rare: An in-frame deletion within the C-terminal FERM domain of CCM1 resulted in decreased protein expression and impaired binding to the transmembrane protein heart of glass (HEG1). Notably, 20% of index cases carrying a CCM mutation were below age 10 and 33% below age 18 when referred for genetic testing. Since fulminant disease courses during the first years of life were observed in CCM1 and CCM3 mutation carriers, predictive testing of minor siblings became an issue.

9.
Mol Cytogenet ; 7(1): 12, 2014 Feb 05.
Article in English | MEDLINE | ID: mdl-24499596

ABSTRACT

BACKGROUND: Meningiomas are mostly benign tumors which arise from the meninges. They are among the cytogenetically best-studied solid tumors, mostly displaying a normal karyotype or, as a typical primary aberration, monosomy of chromosome 22. Further secondary chromosomal aberrations, especially the deletion of chromosome 1p, are correlated with increasing biological aggressiveness up to malignancy. These data are derived from the cytogenetical characterization of 661 meningiomas, from which the genetic progression score (GPS) has been developed. Due to the high expenditure of time and the expert knowledge for the cytogenetical characterization, the aim of this work was to establish an equally reliable yet more rapid clinical diagnosis based on fluorescence in situ hybridization (FISH) on meningiomas. Thus a comparison between the native tumor tissue and the primary culture of the same tumor was done in order to determine the most efficient method for a molecular cytogenetic characterization. The diagnostic procedure has to deliver fast and robust results, since they must enable the attending physician to plan the appropriate follow-up regimens for the patients. All in all, preparations of native tumor tissue as well as preparations of cell culture of 22 meningiomas were tested with FISH for aberrations concerning the prognostically relevant chromosome regions 1p and 9p, and the chromosomes 10, 14, 18 and 22 in comparison with the particular karyotypes revealed by conventional karyotyping using G-banding. RESULTS: The FISH examinations between native and cultured cells showed an accordance of 93.4%. The comparison of FISH data and karyotyping presented accordance to the greatest possible extent concerning the chromosomes 14, 18 and 22, but to detect the progression associated losses of 1p and 9p FISH is the most sensitive method. CONCLUSIONS: The raised data reveal that both methods can be used for a significant analysis of chromosome aberrations on meningiomas. As a result of that the complex primary culture could also be avoided. Therefore a clinical diagnosis based on FISH on meningiomas is at hand for the assignment of patients to a suitable follow-up regimen.

10.
Neurosurg Rev ; 37(1): 161-5, 2014 Jan.
Article in English | MEDLINE | ID: mdl-23722637

ABSTRACT

The mutation detection rate for familial cerebral cavernous malformations (CCM) is extremely high, being about 90 % if direct sequencing of the three genes, CCM1, CCM2, and CCM3, is used in conjunction with quantitative analyses to detect larger CCM1-3 deletions/duplications. We here report on an individual who had presented with more than 30 cerebral and spinal cavernous malformations, two intracranial meningiomas, and disease manifestation only in the mid-forties. A CCM1 missense variant of unclear relevance was found during the first sequencing step. Thereafter, direct sequencing of all three CCM genes revealed the typical pathogenic loss-of-function mutation c.598C > T/p.Q200* in the CCM3 gene. Our results demonstrate that mutation analyses of all three CCM genes in the index patient regardless of previous identification of an unclassified CCM1 variant is crucial for reliable predictive testing of at-risk relatives.


Subject(s)
Hemangioma, Cavernous, Central Nervous System/genetics , Microtubule-Associated Proteins/genetics , Proto-Oncogene Proteins/genetics , Apoptosis Regulatory Proteins/genetics , Carrier Proteins/genetics , DNA Mutational Analysis , Female , Genetic Testing , Genetic Variation , Hemangioma, Cavernous, Central Nervous System/diagnosis , Humans , KRIT1 Protein , Magnetic Resonance Imaging , Membrane Proteins/genetics , Middle Aged , Mutation, Missense , Predictive Value of Tests , Risk Assessment
11.
Muscle Nerve ; 47(1): 127-34, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23169582

ABSTRACT

INTRODUCTION: Reducing body myopathy is a rare X-linked myopathy. It is characterized by intracytoplasmic inclusions that stain with menadione-nitroblue tetrazolium. It is caused by mutations in the FHL1 gene, which encodes the four-and-a-half LIM domain 1 protein (FHL1). METHODS: We performed a clinical, muscle MRI, and histopathological characterization and immunoblot and genetic analysis of the FHL1 protein in a family with 4 individuals affected by reducing body myopathy. RESULTS: We identified a novel missense mutation in FHL1 (c.449G>C; p.C150S). The patients presented with asymmetric proximal weakness and scoliosis. Both of the boys had a more severe course with earlier onset, contractures, and death due to heart failure at 14 and 18 years of age, respectively. MRI revealed fatty infiltration of posteromedial thigh and paraspinal muscles. Histopathological findings showed FHL1-immunoreactive inclusions. Immunoblot analysis revealed a 50% reduction of FHL1 protein. CONCLUSION: In this study we highlighted diagnostic clues in this myopathy and compared our data with the literature.


Subject(s)
Genetic Diseases, X-Linked/genetics , Intracellular Signaling Peptides and Proteins/genetics , LIM Domain Proteins/genetics , Muscle Proteins/genetics , Muscle, Skeletal/pathology , Muscular Diseases/genetics , Adolescent , Genetic Diseases, X-Linked/metabolism , Genetic Diseases, X-Linked/pathology , Genetic Testing , Humans , Intracellular Signaling Peptides and Proteins/metabolism , LIM Domain Proteins/metabolism , Male , Muscle Proteins/metabolism , Muscle, Skeletal/metabolism , Muscular Diseases/metabolism , Muscular Diseases/pathology , Mutation, Missense , Pedigree
13.
Int J Oncol ; 39(6): 1601-8, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21922133

ABSTRACT

Meningiomas arise from the coverings of the brain or the spinal cord. They are mostly benign and can be surgically cured. However, in approximately 5% of the cases, they turn into malignant forms with aggressive clinical behavior and increased risk of tumor recurrence. Cytogenetically meningiomas are well characterized, with normal karyotype or monosomy of chromosome 22 in most tumors and clinically relevant secondary losses of other autosomes and sex chromosomes in a subset of anaplastic tumors. Statistical analyses were performed for 1064 karyotypes derived from 661 meningiomas with respect to progression, and recurrence of the tumor. The order of accumulating genetic aberrations has previously been biostatistically estimated with oncogenetic tree models, and a genetic progression score derived from these models was shown to be predictive for tumor recurrence. Although more homogeneous than other cancer types, meningiomas show considerable intratumoral cytogenetic heterogeneity, particularly in their anaplastic form. We observed different cytogenetic patterns in tumor cells of 224 out of 661 (33.4%) meningiomas. The present study demonstrates that it is not sufficient to consider only the most frequent cytogenetic pattern observed in a sufficient set of cells derived from the same tumor. Even a single cell with more advanced genetic progression may start a clone and indicates also clinical progression. Cox regression analysis reveals that the clone with most advanced progression is a leading marker for recurrence in meningiomas. The aim of this study was the analysis of genetic heterogeneity on single cell basis. Further we investigated if there is a substantial correlation between the intratumoral heterogeneity of a given meningioma and its recurrence risk. We were able to show that the selection of single genetically advanced cells improves the prediction of clinical meningioma progression in a more precise manner.


Subject(s)
Meningeal Neoplasms/diagnosis , Meningeal Neoplasms/genetics , Meningioma/diagnosis , Meningioma/genetics , Neoplasm Recurrence, Local/diagnosis , Adult , Aged , Chromosome Aberrations , Cytogenetic Analysis , Disease Progression , Female , Humans , Kaplan-Meier Estimate , Male , Meningeal Neoplasms/mortality , Meningioma/mortality , Middle Aged , Neoplasm Recurrence, Local/genetics , Prognosis
14.
Eur J Hum Genet ; 19(6): 717-20, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21267006

ABSTRACT

X-linked intellectual disability (XLID), also known as X-linked mental retardation, is a highly genetically heterogeneous condition for which mutations in >90 different genes have been identified. In this study, we used a custom-made sequencing array based on the Affymetrix 50k platform for mutation screening in 17 known XLID genes in patients from 135 families and found eight single-nucleotide changes that were absent in controls. For four mutations affecting ATRX (p.1761M>T), PQBP1 (p.155R>X) and SLC6A8 (p.390P>L and p.477S>L), we provide evidence for a functional involvement of these changes in the aetiology of intellectual disability.


Subject(s)
Carrier Proteins/genetics , DNA Helicases/genetics , Mental Retardation, X-Linked/genetics , Nerve Tissue Proteins/genetics , Nuclear Proteins/genetics , Plasma Membrane Neurotransmitter Transport Proteins/genetics , Polymorphism, Single Nucleotide , Carrier Proteins/biosynthesis , Chromosomes, Human, X/chemistry , DNA Helicases/biosynthesis , DNA-Binding Proteins , Female , Genes, X-Linked , Genetic Association Studies , Genetic Testing , High-Throughput Nucleotide Sequencing , Humans , Hybridization, Genetic , Male , Nerve Tissue Proteins/biosynthesis , Nuclear Proteins/biosynthesis , Pedigree , Plasma Membrane Neurotransmitter Transport Proteins/biosynthesis , Reverse Transcriptase Polymerase Chain Reaction , X-linked Nuclear Protein
17.
Int J Cancer ; 124(2): 346-51, 2009 Jan 15.
Article in English | MEDLINE | ID: mdl-19003955

ABSTRACT

Genome-wide expression signatures improve the understanding of tumor biology. We performed expression profiling of 24 meningioma including 8 of each WHO grade and 2 dura controls analyzing 55.000 transcripts including 18.300 known genes. We compared expression in meningioma vs. dura, expression of low grade (WHO I) vs. higher-grade (WHO II and WHO III) tumors and expression of meningothelial and syncytial meningioma vs. fibroblastic meningioma. Overall expression was significantly decreased in meningioma compared to dura and in meningothelial and syncytial compared to fibroblastic meningioma. Gene expression was exemplarily confirmed by immunohistochemistry using independent samples. Applying our statistical gene set analysis toolkit "GeneTrail", we identified significantly deregulated biochemical pathways using Kyoto encyclopedia of genes and genomes and Transpath databases. Kyoto encyclopedia of genes and genomes pathways with decreased expression in meningioma included cell adhesion molecules (p<0.0001) and cytokine-cytokine receptor interactions (p<0.0001). Pathways with increased expression included several metabolic pathways. Extended expression profiling by a novel statistical gene set enrichment identified pathways that have previously not been associated with meningioma.


Subject(s)
Gene Expression Profiling , Gene Expression Regulation, Neoplastic , Genome , Meningeal Neoplasms/metabolism , Meningioma/metabolism , Cell Adhesion , Computational Biology , Cytokines/metabolism , Expressed Sequence Tags , Humans , Immunohistochemistry/methods , Meningeal Neoplasms/genetics , Meningioma/genetics , Models, Statistical , Oligonucleotide Array Sequence Analysis
19.
Neurosurgery ; 62(1): 61-9; discussion 69-70, 2008 Jan.
Article in English | MEDLINE | ID: mdl-18300892

ABSTRACT

OBJECTIVE: Meningiomas are mostly benign tumors that originate from the coverings of the brain and spinal cord. Cytogenetically, they reveal a normal karyotype or, typically, monosomy of chromosome 22. Progression of meningiomas is associated with a non-random pattern of secondary losses of other autosomes. Deletion of the short arm of one chromosome 1 is a decisive step to anaplastic growth in meningiomas. METHODS: Statistical analyses were performed for the karyotypes of 661 meningiomas with respect to localization, progression, and recurrence of the tumor. A mathematical mixture model estimates typical pathogenetic routes in terms of the accumulation of somatic chromosome changes in tumor cells. The model generates a genetic progression score (GPS) that estimates the prognosis as related to the cytogenetic properties of a given tumor. RESULTS: In 53 patients, one or several recurrences were documented over the period of observation. This corresponds to a total rate of recurrence of 8.0% after macroscopically complete tumor extirpation. Higher GPS values were shown to be strongly correlated with tumor recurrence (P = 2.9 x 10(-7)). High-risk tumors, both in terms of histology and cytogenetics, are localized much more frequently at the brain surface than at the cranial base (P = 1.2 x 10(-5) for World Health Organization grade and P = 3.3 x 10(-12) for GPS categorization). CONCLUSION: The tendency of cranial base meningiomas to recur seems to depend on surgical rather than biological reasons. As a quantitative measure, the GPS allows for a more precise assessment of the prognosis of meningiomas than the established categorical cytogenetic markers.


Subject(s)
Chromosome Aberrations , Meningeal Neoplasms/genetics , Meningeal Neoplasms/pathology , Meningioma/genetics , Meningioma/pathology , Adult , Aged , Cytogenetics , Disease Progression , Female , Follow-Up Studies , Humans , Karyotyping , Male , Meningeal Neoplasms/epidemiology , Meningioma/epidemiology , Middle Aged , Models, Theoretical , Neoplasm Recurrence, Local , Proportional Hazards Models , Retrospective Studies
20.
Eur J Hum Genet ; 16(1): 62-72, 2008 Jan.
Article in English | MEDLINE | ID: mdl-17851451

ABSTRACT

Heterozygous germline mutations in mismatch repair (MMR) genes MLH1, PMS2, MSH2, and MSH6 cause Lynch syndrome. New studies have indicated that biallelic mutations lead to a distinctive syndrome, childhood cancer syndrome (CCS), with haematological malignancies and tumours of brain and bowel early in childhood, often associated with signs of neurofibromatosis type 1. We provide further evidence for CCS reporting on six children from two consanguineous families carrying homozygous PMS2 germline mutations. In family 1, all four children had the homozygous p.I590Xfs mutation. Two had a glioblastoma at the age of 6 years and one of them had three additional Lynch-syndrome associated tumours at 15. Another sibling suffered from a glioblastoma at age 9, and the fourth sibling had infantile myofibromatosis at 1. In family 2, two of four siblings were homozygous for the p.G271V mutation. One had two colorectal cancers diagnosed at ages 13 and 14, the other had a Non-Hodgkin's lymphoma and a colorectal cancer at ages 10 and 11, respectively. All children with malignancies had multiple café-au-lait spots. After reviewing published cases of biallelic MMR gene mutations, we provide a concise description of CCS, revealing similarities in age distribution with carriers of heterozygous MMR gene mutations.


Subject(s)
Adenosine Triphosphatases/genetics , Brain Neoplasms/genetics , Colorectal Neoplasms, Hereditary Nonpolyposis/genetics , DNA Repair Enzymes/genetics , DNA-Binding Proteins/genetics , Germ-Line Mutation , Hematologic Neoplasms/genetics , Neoplastic Syndromes, Hereditary/genetics , Neurofibromatosis 1/genetics , Adolescent , Age of Onset , Child , Consanguinity , DNA Mismatch Repair , Female , Germany , Glioblastoma/genetics , Homozygote , Humans , Infant , Male , Mismatch Repair Endonuclease PMS2 , Pedigree , Syndrome , Turkey/ethnology
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