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1.
Cloning and characterization of 1116NS19.9 heavy and light chain cDNAs and expression of antibody fragments in Escherichia coli.
Tonge, D W; Hennam, J F; Greene, A R; Lee, I D; Edge, M D.
Year Immunol ; 7: 56-62, 1993.
Article in English | MEDLINE | ID: mdl-8372513

Subject(s)
Antibodies, Monoclonal/genetics , Antibodies, Neoplasm/genetics , Escherichia coli/genetics , Genes, Immunoglobulin , Immunoglobulin Heavy Chains/genetics , Immunoglobulin Light Chains/genetics , Peptide Fragments/biosynthesis , Protein Engineering/methods , Recombinant Fusion Proteins/biosynthesis , Amino Acid Sequence , Antibodies, Monoclonal/biosynthesis , Antibodies, Neoplasm/biosynthesis , Base Sequence , Cloning, Molecular , DNA/genetics , Humans , Immunoglobulin Fab Fragments/genetics , Immunoglobulin Fab Fragments/immunology , Immunoglobulin Heavy Chains/biosynthesis , Immunoglobulin Light Chains/biosynthesis , Molecular Sequence Data , Peptide Fragments/genetics , Peptide Fragments/immunology , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/immunology
2.
Expression of eukaryotic coding sequences in Methylophilus methylotrophus.
Hennam, J F; Cunningham, A E; Sharpe, G S; Atherton, K T.
Nature ; 297(5861): 80-2, 1982 May 06.
Article in English | MEDLINE | ID: mdl-6803176

Subject(s)
Cloning, Molecular/methods , Methylococcaceae/genetics , Animals , Chickens , DNA, Bacterial/genetics , DNA, Recombinant , Gene Expression Regulation , Ovalbumin/genetics , Plasmids , Tetrahydrofolate Dehydrogenase/genetics
3.
The concentration of prostaglandin f2alpha in maternal plasma, foetal plasma and amniotic fluid during pregnancy in women.
Johnson, D A; Manning, P A; Hennam, J F; Newton, J R; Collins, W P.
Acta Endocrinol (Copenh) ; 79(3): 589-97, 1975 Jul.
Article in English | MEDLINE | ID: mdl-1173509

ABSTRACT

The concentration of prostaglandin F2alpha has been determined in serial samples of peripheral venous plasma from women at defined times during labour, and studied in detail throughout two consecutive uterine contractions. In addition, the same compound has been measured in single samples of uterine venous plasma, cord venous plasma, and amniotic fluid in groups of patients during early and late pregnancy, labour and at delivery of the baby.


Subject(s)
Amniotic Fluid/analysis , Prostaglandins F/analysis , Female , Fetal Blood , Humans , Labor, Obstetric , Maternal-Fetal Exchange , Pregnancy , Pregnancy Trimester, First , Pregnancy Trimester, Second , Pregnancy Trimester, Third , Progesterone/blood , Prostaglandins F/blood , Uterine Contraction
4.
Further studies on radioimmunoassay systems for plasma oestradiol.
Barnard, G J; Hennam, J F; Collins, W P.
J Steroid Biochem ; 6(2): 107-16, 1975 Feb.
Article in English | MEDLINE | ID: mdl-239274

Subject(s)
Estradiol/blood , Adolescent , Adult , Animals , Antibodies, Anti-Idiotypic , Antibody Specificity , Binding Sites, Antibody , Chemical Precipitation , Estradiol/analogs & derivatives , Estradiol/immunology , Estrogens, Conjugated (USP)/immunology , Female , Humans , Hydrogen-Ion Concentration , Iodine Radioisotopes , Male , Oximes/immunology , Perissodactyla/immunology , Pregnancy , Protein Binding , Rabbits/immunology , Radioimmunoassay/methods , Serum Albumin, Bovine , Succinates/immunology , Temperature , Tritium
5.
A cost reductive method for the radioimmunoassay of plasma androstenedione. A comparison of antisera and the effects of chromatography.
Hennam, J F; Collins, W P.
Acta Endocrinol (Copenh) ; 76(3): 597-607, 1974 Jul.
Article in English | MEDLINE | ID: mdl-4406761

Subject(s)
Androstenedione/blood , Radioimmunoassay , Ammonium Sulfate , Azo Compounds , Calcium , Chromatography, Gel , Cross Reactions , Ethers , Evaluation Studies as Topic , Humans , Immune Sera , Methods , Serum Albumin, Bovine , Succinates , Sulfates , Tritium
6.
The role of prostaglandins in human reproductive function.
Collins, W P; Elias, J A; Hennam, J F; Johnson, D A.
Acta Eur Fertil ; 5(2): 119-36, 1974 Jun.
Article in English | MEDLINE | ID: mdl-4213478

Subject(s)
Prostaglandins/physiology , Reproduction , Administration, Oral , Administration, Topical , Binding Sites , Female , Humans , Injections, Intramuscular , Injections, Intravenous , Male , Pituitary Gland/metabolism , Pregnancy , Prostaglandin Antagonists , Prostaglandins/administration & dosage , Prostaglandins/biosynthesis , Prostaglandins/blood , Prostaglandins/pharmacology , Radioimmunoassay , Reproduction/drug effects
7.
Radioimmunoassay of prostaglandin F-2-alpha in peripheral venous plasma from men and women.
Hennam, J F; Johnson, D A; Newton, J R; Collins, W P.
Prostaglandins ; 5(6): 531-42, 1974 Mar 25.
Article in English | MEDLINE | ID: mdl-4856639

Subject(s)
Prostaglandins/blood , Adult , Animals , Antibodies , Buffers , Chemical Precipitation , Cross Reactions , Evaluation Studies as Topic , Female , Humans , Hydrogen-Ion Concentration , Immune Sera , Labor, Obstetric , Male , Methods , Pregnancy , Rabbits/immunology , Radioimmunoassay , Serum Albumin, Bovine , Solutions , Temperature , Tritium
8.
Radioimmunoassay of plasma testosterone without chromatography: a comparison of four antisera, and the evaluation of a novel approach to liquid scintillation counting.
Tyler, J P; Hennam, J F; Newton, J R; Collins, W P.
Steroids ; 22(6): 871-89, 1973 Dec.
Article in English | MEDLINE | ID: mdl-4769080

Subject(s)
Testosterone/blood , Ammonium Sulfate , Antibody Specificity , Cross Reactions , Humans , Hypogonadism/blood , Immune Sera , Male , Methods , Radioimmunoassay , Serum Albumin, Bovine , Time Factors , Tritium
9.
Proceedings: A cost reductive method for the radioimmunoassay of steroids and prostaglandins.
Hennam, J F; Tyler, J.
J Reprod Fertil ; 35(3): 603-4, 1973 Dec.
Article in English | MEDLINE | ID: mdl-4760172

Subject(s)
Androgens/analysis , Estrogens/analysis , Hydrocortisone/analysis , Progestins/analysis , Prostaglandins/analysis , Ammonium Sulfate , Charcoal , Costs and Cost Analysis , Dextrans , Methods , Radioimmunoassay
10.
Radioimmunoassay of urinary testosterone glucuronoside.
Hennam, J F; Collins, W P; Sommerville, I F.
Steroids ; 21(2): 285-306, 1973 Feb.
Article in English | MEDLINE | ID: mdl-4734715

Subject(s)
Glucuronates/urine , Radioimmunoassay , Testosterone/urine , Adult , Animals , Antibody Specificity , Binding Sites, Antibody , Buffers , Cattle , Chromatography, Gas , Female , Humans , Male , Mathematics , Menstruation , Methods , Nickel , Rabbits/immunology , Radioisotopes , Serum Albumin, Bovine , Time Factors , Tritium
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