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2.
Laryngorhinootologie ; 88(2): 116-8, 2009 Feb.
Article in German | MEDLINE | ID: mdl-19034826

ABSTRACT

The effects of an incorporated button battery as a nasal foreign body with regard to the endonasal structures are serious. The following case report will illustrate this on the case of a 4?-year old boy. The acute treatment consisted of the removal of the battery and the debridement of the necroses. Already these measures required considerable medical expenditure. Reconstructive measures which may be needed in the future, will also require a lot of resources. Button batteries as incorporated nasal foreign bodies can lead to severe damage to the endonasal mucoperichondrium. It can lead to necrosis of the nasal septum cartilage and to consecutive nasal septum perforation. Based on these experiences and a review of literature, the conclusions should be drawn that an incorporated button battery should be considered as an ENT emergency case and the immediate removal of the foreign body should be striven for.


Subject(s)
Burns, Chemical/diagnostic imaging , Electric Power Supplies , Foreign Bodies/diagnostic imaging , Nasal Cavity , Nasal Obstruction/diagnostic imaging , Biopsy , Burns, Chemical/pathology , Burns, Chemical/surgery , Child, Preschool , Debridement , Diagnosis, Differential , Foreign Bodies/pathology , Foreign Bodies/surgery , Humans , Male , Nasal Cavity/diagnostic imaging , Nasal Cavity/injuries , Nasal Cavity/pathology , Nasal Cavity/surgery , Nasal Mucosa/diagnostic imaging , Nasal Mucosa/injuries , Nasal Mucosa/pathology , Nasal Obstruction/pathology , Nasal Obstruction/surgery , Nasal Septum/diagnostic imaging , Nasal Septum/injuries , Nasal Septum/pathology , Necrosis , Radiography
3.
Calcif Tissue Int ; 80(4): 268-74, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17401694

ABSTRACT

Aseptic loosening is the major cause of total joint replacement failure. Substance P (SP) is a neurotransmitter richly distributed in sensory nerve fibers, bone, and bone-related tissue. The purpose of this study was to investigate the potential impact of SP on bone metabolism in polyethylene particle-induced osteolysis. We utilized the murine calvarial osteolysis model based on ultrahigh molecular weight polyethylene (UHMWPE) particles in 14 wild-type mice (C57BL/J6) and 14 SP-deficient mice. Group 1 (C57BL/J 6) and group 3 (SP-knockout) received sham surgery, and group 2 (C57BL/J6) and group 4 (SP-knockout) were treated with polyethylene particles. Analytical methods included three-dimensional micro-computed tomographic (micro-CT) analysis and histomorphometry. Bone resorption was measured within the midline suture. The number of osteoclasts was determined by counting the tartrate-resistant acid phosphatase-positive cells. UHMWPE-particle treated SP-deficient mice showed significantly reduced osteolysis compared to wild-type mice, as confirmed by histomorphometry (P < 0.001) and micro-CT (P = 0.035). Osteoclast numbers were significantly reduced in groups 3 and 4 compared to groups 1 and 2 (P < 0.001). Unexpectedly, SP-deficient mice (group 3) showed a significantly increased absolute bone mass compared to wild-type mice (group 1) (P = 0.02). The findings of our murine calvaria model lead to the assumption that SP is a promoter in particle-induced osteolysis. The pathophysiology of aseptic loosening is complex, and neuropeptides are not solely responsible for the progress of implant loosening; however, we conclude that there could be coherence between neurotransmitters and particle-induced osteolysis in patients with aseptic loosening.


Subject(s)
Bone Resorption/chemically induced , Nanoparticles/therapeutic use , Osteonecrosis/drug therapy , Polyethylenes/therapeutic use , Prosthesis Failure , Substance P/genetics , Animals , Bone Resorption/diagnostic imaging , Bone and Bones/anatomy & histology , Bone and Bones/drug effects , Bone and Bones/metabolism , Male , Materials Testing , Mice , Mice, Inbred C57BL , Mice, Knockout , Osteonecrosis/pathology , Polyethylenes/pharmacology , Skull/diagnostic imaging , Skull/drug effects , Skull/pathology , Substance P/pharmacology , Tomography, X-Ray Computed
4.
Biomed Tech (Berl) ; 50(6): 195-200, 2005 Jun.
Article in German | MEDLINE | ID: mdl-16003921

ABSTRACT

An ongoing unraveling of the molecular mechanisms in aseptic loosening of hip arthroplasty has opened up novel potential pharmacological interventions. In this study the antiresorptive effects of the bisphosphonate zoledronate and the statin simvastatin on ultra high molecular weighted polyethylene (UHMWPE) particle-induced osteolysis were compared. Two previous studies of our group based on the murine calvarial model of UHWMPE particle-induced osteolysis were pooled to form four study groups. Animals in group I (n=14) underwent sham surgery only. In groups II (n=14), III (n=7) and IV (n=7) UHMWPE particles were implanted on the calvariae. Animals in groups III and IV were additionally treated with zoledronate (single 25 microg/kg s.c. injection) and simvastatin (120 mg/day p.o. for 14 days), respectively. After two weeks, calvaria were processed for undecalcified histomorphometry. Bone resorption was measured using Giemsa staining. Osteoclast numbers were determined using TRAP-staining. UHMWPE particle implantation resulted in a grossly pronounced osteolytic activity with significantly increased values of bone resorption (p < 0.001) and osteoclast numbers (p < 0.001). Additional treatment with zoledronate or simvastatin counteracted the particle-induced effects. A comparison of the two medical treatments revealed no statistically significant differences in bone resorption (p = 0.63) and osteoclast numbers (p = 0.41). A single dose of the bisphosphonate zoledronate decreased UHMWPE particle-induced osteolysis in a murine calvarial model as effectively as a daily treatment with simvastin. Both drug groups may have a preventive and therapeutic role as antiresorptive agents in wear particle-induced bone resorption following total joint replacement.


Subject(s)
Bone Resorption/pathology , Bone Resorption/prevention & control , Diphosphonates/therapeutic use , Imidazoles/therapeutic use , Osteolysis/pathology , Osteolysis/prevention & control , Polyethylenes/adverse effects , Simvastatin/therapeutic use , Animals , Bone Resorption/etiology , Female , Foreign-Body Reaction/etiology , Foreign-Body Reaction/pathology , Foreign-Body Reaction/prevention & control , Male , Mice , Mice, Inbred C57BL , Osteolysis/etiology , Particle Size , Skull/drug effects , Skull/pathology , Treatment Outcome , Zoledronic Acid
5.
Z Gastroenterol ; 39(7): 529-32, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11505334

ABSTRACT

Ulcers of the small bowel have repeatedly been described as a late complication of celiac disease and they are considered a signum mali ominis. We report a case of a 53-year-old woman presenting with diarrhea, epigastric pain and abdominal distensions for a period of few weeks. At upper GI endoscopy, biopsies were taken showing complete atrophy of the villi and colonization of the small bowel mucosa. Additionally, uncommon multilocular peptic ulcers were seen in the gastric antrum. These ulcers proved to be Helicobacter pylori-negative with no evidence of Zollinger-Ellison syndrome. Biopsies of gastric ulcers showed signs of a lymphocytic gastritis with an extensive infiltration of the lamina propria by almost exclusively CD3- and CD45R0-positive T-lymphocytes. Intraepithelial T-lymphocytes were found to be increased in the antral as well as the corpus mucosa. Typing the patient for human leukocyte antigens showed a DQA1*0501 and DQB1*0201 phenotype. According to the present report, gastric peptic ulcers seem to be another phenomenon associated with celiac disease. In the case presented here, ulcers were diagnosed together with celiac disease already at first presentation of the patient.


Subject(s)
Celiac Disease/complications , Helicobacter Infections/complications , Helicobacter pylori , Stomach Ulcer/complications , Biopsy , Celiac Disease/pathology , Female , Gastric Mucosa/pathology , Gastritis/complications , Gastritis/pathology , Helicobacter Infections/pathology , Humans , Intestinal Mucosa/pathology , Lymphocytosis/complications , Lymphocytosis/pathology , Middle Aged , Stomach Ulcer/pathology , T-Lymphocytes/pathology
6.
Exp Hematol ; 26(13): 1209-14, 1998 Dec.
Article in English | MEDLINE | ID: mdl-9845376

ABSTRACT

To extend the panel of monoclonal antibodies useful for immunophenotyping of acute leukemias, two new reagents, TC-12 and TH-111, were developed. TC-12 was found "unique," and TH-111 was assigned to the recently defined CD96 cluster. Both reagents show little reactivity with blood and bone marrow nucleated cells but define a major (TH-111: 78.3%) or an important (TC-12: 45.6%) subset of T-cell acute lymphoblastic leukemia (ALL). In addition, in acute myeloid leukemia (AML), the expression of TC-12 was found in 64 (20.2%) of 317 and TH-111 in 97 (29.1%) of 333 of these patients. TC-12 positivity in AML was virtually restricted to the Fab subtypes M0, M1, M2, and M6. In the group of immature AML characterized by the coexpression of CD7 as well as CD117 and CD34 positivity, leukemic blasts frequently disclosed the TC-12 and TH-111 antigen. Although the TC-12 antigen could not be determined, TH-111 immunoprecipitated the TACTILE (CD96) antigen and, when expressed, was found to be associated with the transferrin receptor. These reagents may help not only to define and dissect T-cell ALL, but also to characterize a subgroup of immature AML at the divergence of T-cell and myeloid lineage.


Subject(s)
Antibodies, Monoclonal , Antigens, CD/immunology , Immunophenotyping , Leukemia, Myeloid, Acute/immunology , Leukemia-Lymphoma, Adult T-Cell/immunology , Animals , Antibody Specificity , Antigens, CD/analysis , Antigens, Neoplasm/analysis , Antigens, Neoplasm/immunology , Female , Humans , Hybridomas/immunology , Immunosorbent Techniques , Indicators and Reagents , Mice , Mice, Inbred BALB C , Tumor Cells, Cultured
7.
Am J Med Genet ; 63(1): 50-4, 1996 May 03.
Article in English | MEDLINE | ID: mdl-8723086

ABSTRACT

Geleophysics dysplasia, a rare disorder with autosomal-recessive inheritance, is characterized by short stature with a "happy-looking" facial appearance. Nonskeletal findings, particularly in an advanced stage, include hepatosplenomegaly and valvular cardiopathy. Based on the clinical picture and the detection of lysosome-like inclusions in hepatocytes, the underlying cause of the condition is considered to be a storage defect in the metabolism of glycoproteins. The clinical course, with progressive worsening of the condition favors this hypothesis. We report on 3 further cases, in which light and electron microscopic studies of iliac crest biopsies and cultured skin fibroblasts provided additional evidence that geleophysic dysplasia represents a lysosomal storage disease. The additional discovery of storage vacuoles in chondrocytes and skin fibroblasts strongly suggests that the condition is a generalized storage defect. To date, it has not yet been possible to identify the presumed biochemical defect in the metabolic pathways of glycoproteins.


Subject(s)
Osteochondrodysplasias/genetics , Osteochondrodysplasias/pathology , Bone and Bones/diagnostic imaging , Child , Female , Genes, Recessive , Humans , Ilium/pathology , Ilium/ultrastructure , Male , Microscopy, Electron , Nuclear Family , Osteochondrodysplasias/diagnostic imaging , Radiography
8.
Cancer Genet Cytogenet ; 61(1): 6-10, 1992 Jul 01.
Article in English | MEDLINE | ID: mdl-1638481

ABSTRACT

Several subclones were identified in unstimulated peripheral blood cells from a patient with chronic myeloproliferative disease, which was classified as myelofibrosis by morphologic terms. These subclones were characterized by an unusual number of different karyotype anomalies. Some of the more complex chromosomal rearrangements could be clearly defined by fluorescence in situ hybridization. Chromosome arms involved in clonal aberrations were 1q, 3p, 6p, 7q, 11q, 13q, 15q, 17q, 18p, and 20q. Reconstruction of karyotype evolution was attempted by karyotypic analysis of 100 metaphase spreads each in two separate investigations.


Subject(s)
Chromosome Aberrations , Primary Myelofibrosis/genetics , Aged , Chromosome Banding , Female , Humans , Karyotyping , Primary Myelofibrosis/pathology
9.
Ann Hematol ; 63(1): 20-6, 1991 Jul.
Article in English | MEDLINE | ID: mdl-1908707

ABSTRACT

Two new monoclonal antibodies, EBU-65 and EBU-141, were raised by immunization with plasma cell line U-266. Both antibodies strongly react with B lymphocytes in immunofluorescent staining as well as on paraffin-embedded sections. More than 200 leukemias and lymphomas were tested, and for both antibodies reactivity was found only with "mature" B-cell tumors but not with precursor B-cell leukemias. None of the non-B-lineage hematolymphatic tumors tested was stained by EBU-141 or EBU-65. A subpopulation of T lymphocytes particularly present in nonmalignant pleural effusions was detected by EBU-65 additionally. Although EBU-141 was clustered as CDw75 and EBU-65 as "unique," a close relationship of the staining pattern was found and both antibodies react with a sialyltransferase. In particular, CDw75 antibody EBU-141 was demonstrated to be very useful for immunophenotyping of B-cell neoplasias, while EBU-65 reacted with most multiple myelomas and a subgroup of "activated"-appearing T cells.


Subject(s)
Antibodies, Monoclonal/immunology , B-Lymphocytes/immunology , Leukemia/immunology , Lymphoma/immunology , Animals , Antigens/immunology , Callitrichinae , Cell Line , Female , Fluorescent Antibody Technique , Haplorhini , Humans , Hylobates , Immunophenotyping , Mice , Mice, Inbred BALB C , Plasma Cells/immunology , Saimiri
10.
Z Rheumatol ; 50(2): 103-8, 1991.
Article in German | MEDLINE | ID: mdl-1872041

ABSTRACT

The concentration of 6-methoxy-2-naphthyl acetic acid (6-MNA) in plasma, synovial fluid, synovial tissue and fibrous capsule tissue was determined in an open study with 20 patients scheduled for knee joint surgery after oral treatment with nabumetone (Arthaxan) under steady state conditions. 6-MNA is the principal metabolite of the prodrug nabumetone arising from an extensive first-pass metabolism in the liver. The patients suffering from rheumatoid arthritis (n = 12) or osteoarthritis stage III or IV (n = 8) received a daily dose of 1 g nabumetone nocte starting 4 days prior to surgery. On day 1 an additional loading dose of 1 g nabumetone was given in the morning. At the time of surgery (day 5) simultaneously blood and synovial fluid was aspirated and after medial opening of the knee joint biopsies of synovial tissue and fibrous capsule tissue were taken. The samples were analysed employing HPLC. After 4 days of treatment mean 6-MNA concentration in plasma was 40.76 micrograms/ml, in synovial fluid 34.79 micrograms/ml, in synovial tissue 19.33 micrograms/g and in fibrous capsule tissue 11.43 micrograms/g. Under steady state conditions mean synovial fluid levels of 6-MNA were higher than after application of a single dose.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal , Arthritis, Rheumatoid/drug therapy , Butanones/therapeutic use , Knee Joint , Naphthaleneacetic Acids/pharmacokinetics , Osteoarthritis/drug therapy , Synovial Fluid/metabolism , Adult , Aged , Arthritis, Rheumatoid/blood , Biological Availability , Biotransformation , Butanones/pharmacokinetics , Drug Administration Schedule , Female , Humans , Knee Joint/drug effects , Knee Joint/metabolism , Male , Middle Aged , Nabumetone , Osteoarthritis/blood
12.
Drugs ; 40 Suppl 5: 57-61, 1990.
Article in English | MEDLINE | ID: mdl-2081495

ABSTRACT

The concentration of 6-methoxy-2-naphthylacetic acid (6-MNA) in plasma, synovial fluid, synovial tissue and fibrous capsule tissue was determined in an open study with 20 patients scheduled for knee joint surgery after oral treatment with nabumetone under steady-state conditions. 6-MNA is the principle metabolite of the prodrug nabumetone arising from an extensive first-pass metabolism in the liver. Patients suffering from rheumatoid arthritis (n = 12) or osteoarthritis stage III or IV (n = 8) received a daily dose of nabumetone 1 g in the evening starting 4 days prior to surgery. On day 1 an additional loading dose of nabumetone 1 g was given in the morning. At the time of surgery (day 5), blood, synovial tissue and fibrous capsule tissue were taken simultaneously. The samples were analysed by high performance liquid chromatography. After 4 days of treatment mean 6-MNA concentration in plasma was 40.76 mg/L, in synovial fluid 34.79 mg/L, in synovial tissue 19.33 mg/g and in fibrous capsule tissue 11.43 mg/g. Under steady-state conditions mean synovial fluid levels of 6-MNA were higher than after administration of a single dose and, in common with levels in synovial tissue, persist in a range sufficient for in vitro cyclo-oxygenase inhibition.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/metabolism , Butanones/metabolism , Naphthaleneacetic Acids/metabolism , Administration, Oral , Adult , Aged , Anti-Inflammatory Agents, Non-Steroidal/analysis , Anti-Inflammatory Agents, Non-Steroidal/blood , Arthritis, Rheumatoid/drug therapy , Arthritis, Rheumatoid/metabolism , Butanones/analysis , Butanones/blood , Chromatography, High Pressure Liquid , Female , Humans , Knee Joint/surgery , Male , Middle Aged , Nabumetone , Naphthaleneacetic Acids/analysis , Naphthaleneacetic Acids/blood
14.
J Rheumatol ; 15(3): 500-4, 1988 Mar.
Article in English | MEDLINE | ID: mdl-3164075

ABSTRACT

Rheumatic symptoms in patients with leukemia are not uncommon. Often they delay correct diagnosis and therapy. We describe a patient presenting with oligoarticular joint disease in whom immunological analysis of synovial fluid (SF) led to the detection of leukemic cells in the joint. Moreover, analysis with a variety of monoclonal antibodies established the diagnosis of acute lymphoblastic leukemia of null cell type with phenotypically identical malignant cells present in the bone marrow, peripheral blood and SF. Our investigations demonstrate that analysis with monoclonal antibodies is helpful in characterizing joint involvement in patients with leukemia.


Subject(s)
Antibodies, Monoclonal , Leukemia, Lymphoid/pathology , Synovial Fluid/cytology , Acute Disease , Adolescent , Blood Cell Count , Bone Marrow/pathology , Humans , Immunohistochemistry , Joints/physiopathology , Leukemia, Lymphoid/diagnosis , Leukemia, Lymphoid/physiopathology , Male , Pain
15.
Dtsch Med Wochenschr ; 111(4): 139-41, 1986 Jan 24.
Article in German | MEDLINE | ID: mdl-3510849

ABSTRACT

In a 46-year-old female patient suffering from renal hypertension due to polycystic kidney degeneration with creatinine values between 3 and 5 mg/dl leucopenia developed 3 weeks after beginning antihypertensive therapy with the angiotensin-converting-enzyme inhibitor captopril. On continuation of treatment signs of haemorrhagic diathesis appeared 7 months later. Cytology and histology led to the diagnosis of panmyelopathy which, from the clinical findings and the close patient follow-up, was to be regarded as captopril-induced. Bone-marrow regeneration could be shown 6 months after discontinuing the drug. In renal insufficiency, captopril treatment should be monitored using regular haematological tests and the drug withdrawn when leuco- or thrombocytopenia occurs.


Subject(s)
Bone Marrow Diseases/chemically induced , Captopril/adverse effects , Bone Marrow/drug effects , Bone Marrow/physiology , Captopril/therapeutic use , Female , Humans , Hypertension, Renal/drug therapy , Middle Aged , Regeneration
16.
Morphol Med ; 3(3): 157-63, 1983 Oct.
Article in German | MEDLINE | ID: mdl-6664377

ABSTRACT

Computed tomography, a relatively young X-ray imaging modality, has so far been mainly employed for the differentiation and densitometry of soft tissues. By modification of the image reconstruction algorithm spatial resolution was improved significantly such that imaging of fine structures and of high contrast boundaries became possible. The image characteristics of the high-resolution computed tomograms were investigated on petrous bones and lumbar vertebral bodies. In the petrous bone, an exact anatomic reconstruction of the bony structures of the inner ear is achieved. In vertebral bodies, imaging of spongiosa structures with 2 mm slice thickness and thereby an early diagnosis of osteoporosis are possible.


Subject(s)
Bone and Bones/diagnostic imaging , Tomography, X-Ray Computed , Humans , Lumbar Vertebrae/diagnostic imaging , Mastoiditis/diagnostic imaging , Osteoporosis/diagnostic imaging , Petrous Bone/diagnostic imaging
18.
Pathol Res Pract ; 173(4): 359-68, 1982.
Article in German | MEDLINE | ID: mdl-7122327

ABSTRACT

In two healthy and two diseased whitefish (Coregonus Wartmanni) taken from Lake Constance (FRG), ankylosis of the vertebral column was investigated both roentgenologically and histologically. Subsequent to the collapse and necrosis of the "residual" spinal cord within the intervertebral spaces, the outside edges of the vertebral bodies come into direct contact. The compression and tensile forces that occur to an increased extent as a result of the instability, lead not only to a remodelling of the vertebral bodies, but also to the formation of spondylotic osteophytes at the edges of the vertebrae and, as a result of periosteal stimulation, to the development of cellular hyaline cartilage, which fills the intervertebral spaces. Finally, as a result of perichondral ossification, a bony ankylosis develops. The humping of the spine of the fish due to the stiffening and shortening of the vertebral column, is accompanied by a restriction in the animal's freedom of movement. Muscular atrophic processes and disordered food uptake give rise to poor growth and a reduction in the weight of the diseased fish. These remodelling processes in the spine resulting from instability are specific to the periosteum and may be equated with the changes seen in man in spondylosis deformans. The possible cause of this vertebral column ankylosis is cadmium poisoning. The accumulation of this heavy metal obviously leads primarily to an irreversible toxic degeneration of the cells of the chorda dorsalis.


Subject(s)
Ankylosis/veterinary , Cadmium Poisoning/veterinary , Fish Diseases/pathology , Salmonidae , Spinal Diseases/veterinary , Animals , Ankylosis/pathology , Cadmium Poisoning/pathology , Female , Spinal Diseases/pathology , Spine/pathology
20.
Microsc Acta Suppl ; Suppl 4: 124-8, 1980.
Article in German | MEDLINE | ID: mdl-6995796

ABSTRACT

In 114 autopsy cases of both sexes, aged between 31 and 97 years and without bone disease, the influence of mechanical forces on the age-dependent remodelling of the spongy bone was determined in the 3rd and 5th lumbar vertebrae and the neck of the femur. For this purpose contrasty X-ray images of 100 mu thick polished bone sections were analysed using the LEITZ texture analysis system. The volumetric density and the surface density are highest in the neck of the femur, lowest in the 3rd lumbar vertebra, and almost as low in the 5th lumbar vertebra. The volumetric density decreases with increasing age by about one-third in all three bones. Correspondingly, the surface density also decreases in the lumbar vertebrae by one-third, but only by 18% in the neck of the femur. The specific surface reveals no age differences in the lumbar vertebrae, but increases by 19% in the neck of the femur. The constancy of the specific surface in the lumbar vertebrae can be explained by the fact that compensatory hypertrophy of the remaining trabeculae takes place. This compensatory growth of bone does not occur in the neck of the femur. The age-dependent decrease in spongy bone manifests itself in a residual spongiosa structure that depends on compressive forces in the case of the lumbar vertebrae and bending forces in the neck of the femur.


Subject(s)
Aging , Femur/anatomy & histology , Histological Techniques , Lumbar Vertebrae/anatomy & histology , Adult , Aged , Female , Femur/diagnostic imaging , Femur/physiology , Humans , Lumbar Vertebrae/diagnostic imaging , Lumbar Vertebrae/physiology , Male , Middle Aged , Radiography
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