ABSTRACT
Ammonia injury is an uncommon injury, but it is associated with high morbidity and mortality. This case report demonstrates the pathophysiology and treatment of both cutaneous burn wounds and inhalation injury caused by ammonia. Frequent bronchoscopy was used to attempt to avoid intubation and its associated morbidity. The patient remained extubated, but later he required skin grafts to close his wounds after healing of his pulmonary injury. A review of the management of inhalation injury is also discussed. Ammonia injury can cause a severe inhalation injury. Bronchoscopy can be a useful tool to avoid intubation.
Subject(s)
Ammonia/adverse effects , Burns, Chemical/etiology , Burns, Inhalation/etiology , Inhalation Exposure/adverse effects , Adult , Burns, Chemical/physiopathology , Burns, Chemical/therapy , Humans , MaleABSTRACT
Thrombospondin-1 (TSP-1), an adhesive glycoprotein, plays an important role in platelet adhesion, inflammation, cell-to-cell interaction, and angiogenesis. TSP-1 is expressed by endothelial cells, fibroblasts, and macrophages. TSP-1's unique cysteine-serine-valine-threonine-cysteine-glycine (CSVTCG) specific receptor plays an important role in the binding and modulation of cellular adhesion and invasion. This article histologically and quantitatively evaluates TSP-1 and its CSVTCG receptor in adult burn wounds over time. Tissue was obtained from burn wounds on several days and samples that were 5 microns thick were placed on slides. Expression of TSP-1 and its CSVTCG receptor were evaluated immunohistochemically and quantitated by computer image analysis in units of absorbance. Immunoglobin G (IgG) (negative) controls were performed and subtracted from the TSP-1 sample to eliminate background absorbance readings. Serum (negative) control was used for the CSVTCG receptor. Platelet concentrates were used as the positive control. A quantitative examination of the results yielded the following information, expressed as absorbance +/- standard error of the mean: TSP-1: day 1, 62.0 +/- 10.13; day 3, 76.2 +/- 6.90; day 5, 36.0 +/- 3.96; day 7, 60.4 +/- 5.67; and day 9, 29.5 +/- 2.91. TSP-1 displays an early peak, followed by a steep decrease over the time period studied. The readings for the CSVTCG receptor are as follows: day 1, 33.8 +/- 1.87; day 3, 34.5 +/- 5.39; day 7, 39.1 +/- 1.93; day 21, 39.1 +/- 1.93; day 28, 34.8 +/- 3.67. In contrast, the CVSTCG receptor continues to be present in the wound over time. Histologic findings are reported, and photographs and a histopathologic analysis are included. The information presented in this article leads to the conclusion that temporal and histologic differences exist in the localization and expression of TSP-1 and its CSVTCG receptor. TSP-1 is up-regulated in injured tissues immediately after the injury; it is rapidly down-regulated as the tissue heals. In contrast, the levels of the CSVTCG receptor remain relatively constant during the healing process. These data are consistent with TSP-1's known role in cell-to-cell interaction, including the modulation of the growth factor and protease activity.
Subject(s)
Burns/pathology , Receptors, Cell Surface/analysis , Thrombospondin 1/analysis , Adult , Awards and Prizes , Burns/physiopathology , Cell Division/physiology , Culture Techniques , Humans , Image Interpretation, Computer-Assisted , Immunohistochemistry , Injury Severity Score , Netherlands , Receptors, Cell Surface/metabolism , Reference Values , Skin/pathology , Thrombospondin 1/metabolism , Wound Healing/physiologyABSTRACT
After a burn injury, the hemodynamics of a patient is changed. There is usually a fall in hematocrit. In addition to this, there is the loss of blood during the grafting procedure. Some patients cannot tolerate this loss of blood. The method we use to help decrease the loss of blood during skin grafting is an injection of epinephrine intradermally before the graft and eschar are excised. We have found this method to be useful in a select group of patients.
