ABSTRACT
The effect of sodium fluoride and sodium monofluorophosphate at concentrations of 1, 5, 10, 50, 100 and 1000 mg/l in phosphate buffer (pH 6.5) as well as in UHT milk were studied on cultures and suspensions of Streptococcus mutans, Lactobacillus acidophilus and Candida albicans. Using serial tenfold dilutions up to 10(-7) of 24-48 hour cultures, a subsequent 0, 60 and 120 min incubation caused no decrease in the number of CFUs. Growth kinetic studies in the Bioscreen biophotometer (Labsystem, Finland) revealed that sodium fluoride in different concentrations (from 0.875 mg/l up to 500 mg/l) influenced the growth dynamics of S. mutans and C. albicans: the exponential phase flattened out at the highest fluoride concentrations (500 mg/l) present in the growth media. The lag phase of C. albicans became longer. The results of these experiments indicate that sodium fluoride administered at higher concentrations than the usual caries preventive dosage made the generation time of cariogenic oral bacteria and fungi longer, slowing down their multiplication.
Subject(s)
Candida albicans/drug effects , Fluorides/pharmacology , Lactobacillus acidophilus/drug effects , Phosphates/pharmacology , Sodium Fluoride/pharmacology , Streptococcus mutans/drug effects , Animals , Buffers , Candida albicans/growth & development , Colony Count, Microbial , Dental Caries/microbiology , Humans , Lactobacillus acidophilus/growth & development , Microbial Sensitivity Tests/methods , Milk , Streptococcus mutans/growth & developmentABSTRACT
The development of oral microbiology at the Semmelweis University of Medicine started around 1980 in the Institute of Microbiology. The teaching of oral microbiology was introduced to the dental educational program, basic research was initiated, appropriate methods learned and used. An intensive collaboration with the Department of Conservative Dentistry developed, which resulted between 1983 and 1995 in about 23 publications (the majority in international journals) and eight lectures, mainly on international forums.
Subject(s)
Mouth Diseases/microbiology , Research , Schools, Medical , Humans , HungaryABSTRACT
There are no significant differences between the microflora of root surface caries and enamel caries. However, intact root surfaces and active lesions may show differences in the proportion of microorganisms. S. mutants together with Lactobacillus on the same surface indicate a higher risk for root surface caries.
Subject(s)
Dental Caries/microbiology , Tooth Root/microbiology , Dental Caries Susceptibility , Dental Enamel/injuries , Dental Enamel/microbiology , Female , Humans , Male , Toothbrushing/adverse effectsABSTRACT
AmF and SnF2 containing toothpastes (equally 750 ppm F-) and mouthrinses (equally 125 ppm F-) from the GABA International Agency were tested. After a five month treatment period (daily two times tooth cleaning), in the saliva of the examined persons the germ counts of the S. mutans and Lactobacillus showed a decreasing tendency, though the change was not statistically significant. The control group used NaF containing cleaners (toothpaste; 1500 ppm F-, mouthrinse; 250 ppm F-) for also a five month treatment period. Within this group the germ counts of the microorganisms of the examined saliva did not change markedly.
Subject(s)
Fluorides/pharmacology , Mouth Mucosa/microbiology , Mouthwashes/pharmacology , Tin Fluorides/pharmacology , Toothpastes/pharmacology , Amines , Humans , Mouth Mucosa/drug effectsABSTRACT
In the course of the study the photopolimerizating filling materials (Visiomolar, Heliomolar, Heliosit, Silux, P-50, Prismatic) and the glass ionomer cements (Ketacfil, Ketac-silver) served as supposed sources of carbon for Ps. aeruginosa. All the examined materials have shown a suitable resistance to the studied bacterium species. If sunflower oil has been absorbed onto the surface of the examined materials, then a limited increase of the living germ counts has been observed. Ketac-silver was proved to be bactericidal in every case.
