Human-animal interactions and safety during dairy cattle handling--Comparing moving cows to milking and hoof trimming.

Cattle handling is a dangerous activity on dairy farms, and cows are a major cause of injuries to livestock handlers. Even if dairy cows are generally tranquil and docile, when situations occur that they perceive or remember as aversive, they may become agitated and hazardous to handle. This study aimed to compare human-animal interactions, cow behavior, and handler safety when moving cows to daily milking and moving cows to more rarely occurring and possibly aversive hoof trimming. These processes were observed on 12 Swedish commercial dairy farms. The study included behavioral observations of handler and cows and cow heart rate recordings, as well as recording frequencies of situations and incidents related to an increased injury risk to the handler. At milking, cows were quite easily moved using few interactions. As expected, the cows showed no behavioral signs of stress, fear, or resistance and their heart rate only rose slightly from the baseline (i.e., the average heart rate during an undisturbed period before handling). Moving cows to hoof trimming involved more forceful and gentle interactions compared with moving cows to milking. Furthermore, the cows showed much higher frequencies of behaviors indicative of aversion and fear (e.g., freezing, balking, and resistance), as well as a higher increase in heart rate. The risk of injury to which handlers were exposed also increased when moving cows to hoof trimming rather than to routine milking. Some interactions (such as forceful tactile interactions with an object and pulling a neck strap or halter) appeared to be related to potentially dangerous incidents where the handler was being kicked, head-butted, or run over by a cow. In conclusion, moving cows to hoof trimming resulted in higher frequencies of behaviors indicating fear, more forceful interactions, and increased injury risks to the handler than moving cows to milking. Improving potentially stressful handling procedures (e.g., by better animal handling practices and preparation of cows to cope with such procedures) can increase handler safety, animal welfare, ease of handling, and efficiency.

Development of automatic surveillance of animal behaviour and welfare using image analysis and machine learned segmentation technique.

In this paper the feasibility to extract the proportion of pigs located in different areas of a pig pen by advanced image analysis technique is explored and discussed for possible applications. For example, pigs generally locate themselves in the wet dunging area at high ambient temperatures in order to avoid heat stress, as wetting the body surface is the major path to dissipate the heat by evaporation. Thus, the portion of pigs in the dunging area and resting area, respectively, could be used as an indicator of failure of controlling the climate in the pig environment as pigs are not supposed to rest in the dunging area. The computer vision methodology utilizes a learning based segmentation approach using several features extracted from the image. The learning based approach applied is based on extended state-of-the-art features in combination with a structured prediction framework based on a logistic regression solver using elastic net regularization. In addition, the method is able to produce a probability per pixel rather than form a hard decision. This overcomes some of the limitations found in a setup using grey-scale information only. The pig pen is a difficult imaging environment because of challenging lighting conditions like shadows, poor lighting and poor contrast between pig and background. In order to test practical conditions, a pen containing nine young pigs was filmed from a top view perspective by an Axis M3006 camera with a resolution of 640 × 480 in three, 10-min sessions under different lighting conditions. The results indicate that a learning based method improves, in comparison with greyscale methods, the possibility to reliable identify proportions of pigs in different areas of the pen. Pigs with a changed behaviour (location) in the pen may indicate changed climate conditions. Changed individual behaviour may also indicate inferior health or acute illness.

Short communication: effect of alley floor cleanliness on free-stall and udder hygiene.

The objective was to evaluate the effect of alley floor cleanliness on the hygiene of the free-stalls and udder and teats of cows. Mechanical scrapers were used on rubber-slatted floors to improve the cleanliness of the alley floor. Two sections containing 21 cows each were evaluated once weekly for a period of 3 wk. One section had scrapers on the rubber-slatted floor in the alleys and the other section did not. The scrapers ran 12 and 7 times/d in the free-stall alley and feed alley, respectively. Manure accumulation in the alleys at approximately 2 h after the scrapers had run was less in the section with the scraper than in that without the scraper. Differences between sections with and without scrapers were found in the sites close to the wall in the feed alley, and immediately behind the free-stalls in the free-stall alley. In the section without scrapers, manure accumulation was greater in the free-stall alley than in the feed alley. Manure contamination of the area in the free-stalls where the udder normally comes in contact with the free-stall floor was significantly reduced from 29.1 to 17.9 +/- 2.4 g of dry matter when the alley floor was kept clean by the use of scrapers. The hygiene scores of the dirtiness of udders were reduced from 25.8 +/- 1.7 to 18.9 +/- 1.6, and those of the teats from 37.9 +/- 2.3 to 24.2 +/- 2.3 in the section with scrapers and cleaner alley floor compared with the section without scrapers. The scrapers greatly improved the hygiene on the rubber-slatted alley floor. The cleaner alley floor had a positive effect on the cleanliness of the free-stalls and the udder and teats of the cows.

Investigating locomotion of dairy cows by use of high speed cinematography.

The longterm influence of management systems on the locomotion of 17 dairy cows was investigated by high speed cinematography (100 frames/s) and kinematic analysis. Angular patterns and hoof trajectories of the left fore- and hindlimbs are presented and statistics made of occurring minimum and maximum angles. At the recording, 3 cows had been kept in tie-stalls (TI) and 6 cows in cubicles (CI) for a consecutive time of about 2.5 years while 8 cows had been kept on grass for about 3 months. Four of the grazing cows had earlier been kept in cubicles (CG) and 4 in tie-stalls (TG) during earlier off grazing seasons together with TI and CI cows. The CI cows had a smaller maximum angle of the elbow joint compared to TI, TG and CG cows. The hock joint angle of the CI cows was less flexed during the stance phase than in TI and CG cows while the minimum angle during the swing phase was greater in the TI and CI cows compared to TG and CG cows. Pastured cows (TG and CG) had a less pronounced flexion of the fetlock joint angle during the stance compared to cows kept indoors (TI and CI). The results suggest that slatted floor and lack of exercise during summer grazing may affect locomotion. This is indicated by restrictions in the movements of the elbow and hock joints and in less fetlock joint flexion at full support.

Sole haemorrhages and heel horn erosion in dairy cows: the influence of housing system on their prevalence and severity.

Altogether 94% of the primiparous and 66% of the multiparous cows had haemorrhages of the sole at trimming 2-4 months after calving. Within cows, and independent of age and stall system, the lateral hind claws had higher scores of haemorrhages than the other claws. The primiparous cows had higher scores than the multiparous cows both in the tie-stalls and in the cubicles. The palmar/plantar region of the claw capsule was divided into zones ("white line zones" and "sole zones"). For the multiparous cows the haemorrhage scores of the "white line zones" were higher among the cows housed in the cubicles than among those housed in the tie stalls. There was no difference between stall systems for haemorrhages of the "sole zones". Heel horn erosion was absent or rare in the fore hooves of both the primiparous and multiparous cows in the tie-stalls. The cows in the cubicles had higher heel horn erosion scores in their fore hooves than the cows in the tie-stalls. The scores of the hind hooves were generally higher than those of the fore hooves in both systems. Heel horn erosions were reduced in association with the introduction of a footbath. A higher incidence of lameness was observed in the cubicle system.

Increased lithogenicity of the bile after jejunoileal bypass in the rat.

Increased formation of biliary calculi is a complication of jejunoileal bypass (JIB) in man. We investigated the biliary lipid composition and bile flow rate in the rat 42 days after a JIB or sham operation. In the bile of the bypassed rats, the percentage cholesterol saturation and the concentration of phospholipids were significantly increased, while the concentration of bile acids and the lithogenic index (bile acids + phospholipids/cholesterol) were markedly decreased. The concentrations of cholesterol in the bile and in the liver were unchanged. These results suggest an increase in lithogenicity of the bile with decreased bile flow after JIB in the rat.

Effect of jejunoileal bypass on plasma and brain amino acids in the rat.

Liver failure and coma are serious complications of jejunoileal bypass (JIB) in man. Rats underwent either a 90-95% JIB or a sham operation. Six weeks later all animals were sacrificed, and plasma and brain amino acids were determined. In the plasma of rats with JIB compared with sham operation, the concentrations of valine, leucine, isoleucine, lysine, tryptophan, and tyrosine were significantly lower, while in the brain, phenylalanine, tyrosine, histidine, and glutamine were significantly higher. These changes in the brain are similar to those resulting from portalsystemic shunting in the rat.

Circulating immunoreactivities of gastrin, glucagon and VIP after jejuno-ileal bypass.

Seven Sprague-Dawley rats (404-440 g) underwent a 90% jejuno-ileal bypass (JIB); the functional loop consisted of 1/3 ileum and 2/3 jejunum with the bypassed loop being anastomosed to the ascending colon. Seven control rats were sham-operated. After 35 days, the rats were fasted 18 hours and venous blood was collected. Immunoreactivity of gastrin, measured with an antibody binding equally to G17 and G34, was higher in the plasma of the JIB (256 +/- 55 SEM pg/ml) than control (85 +/- 9 pg/ml) rats. This agrees with recent human studies but is in conflict with results in less mature rats. VIP levels were not significantly different. Glucagon-like immunoreactivity measured with antibodies specific for the C- and N-terminal regions of the hormone, respectively, were also higher in the JIB (510 +/- 40 and 129 +/- 15 pg/ml) rats.

Kinetic study of serum penicillin concentrations after single doses of benzathine and benethamine penicillins in young and old people.

In a comparative kinetic study of the serum concentrations of two penicillin complexes--medium-long-acting (benethamine penicillin) and long-acting (benzathine bipenicillin)--after a single injection in young adults and elderly people, the following results were confirmed statistically: (a) age was a major factor in the variations in serum penicillin concentrations and in their persistence in the serum; (b) the penicillin was absorbed faster in young than in elderly subjects even when a long-acting complex was used; (c) serum concentrations below the level regarded as lethal for treponemes appeared much earlier and more frequently in young than in old people; and (d) the bioequivalence between penicillin preparations could not be estimated solely for the number of units of the agent used but from the bioavailability of the chosen formulation. Thus a uniform and standard penicillin dosage allowing no safety margin may help in the superficial healing of a syphilitic chancre or the resolution of a roseola but it will certainly be insufficient to kill Treponema pallidum. It seems essential therefore to provide an antibiotic cover at high dosage over a long period of time.