1.
J Dairy Res
; 68(3): 429-36, 2001 Aug.
Article
in English
| MEDLINE
| ID: mdl-11694045
ABSTRACT
Specific PCR, amplifying a fragment of the mitochondrially encoded cytochrome b gene, are developed for discrimination of chicken, turkey, pig, cow and sheep. These PCR tests could be applied for detection and discrimination of animal food ingredients. For origin identification of milk, the bovine PCR is applied on DNA extracted with the DNeasy Tissue kit. A PCR of 30 cycles reached a sensitivity of 720 somatic cells in the PCR reaction corresponding to 29 x 10(4) ells per ml milk. A PCR of 40 cycles could detect 0.72 to 0.144 somatic cells in the PCR reaction corresponding to 29 to 2.9 cells per ml milk.
Subject(s)
Cytochrome b Group/genetics , Food Analysis , Milk/chemistry , Polymerase Chain Reaction/veterinary , Animals , Base Sequence , Cattle/genetics , Chickens/genetics , Cytochrome b Group/isolation & purification , DNA/analysis , DNA/chemistry , Food Analysis/methods , Molecular Sequence Data , Polymerase Chain Reaction/methods , Sensitivity and Specificity , Sequence Alignment , Sheep/genetics , Species Specificity , Swine/genetics , Turkeys/genetics
2.
J Lanc Cty Hist Soc
; 102(2-3): 114-34, 2000.
Article
in English
| MEDLINE
| ID: mdl-17183761
Subject(s)
Almshouses , Social Welfare , Almshouses/ethics , Almshouses/history , Almshouses/legislation & jurisprudence , Almshouses/methods , Almshouses/standards , Almshouses/statistics & numerical data , Architecture/ethics , Architecture/history , Architecture/legislation & jurisprudence , Architecture/methods , History, 18th Century , History, 19th Century , History, 20th Century , Pennsylvania , Social Welfare/classification , Social Welfare/economics , Social Welfare/history , Social Welfare/legislation & jurisprudence , Social Welfare/psychology , Workforce
3.
Pa Folklife
; 25(4): 16-25, 1976.
Article
in English
| MEDLINE
| ID: mdl-11632649
