[Monoclonal antibodies which recognize herpes simplex virus and their possible diagnostic application]. / Anticuerpos monoclonales (AcM) que reconocen el virus herpes simple (HSV) y su posible aplicación al diagnóstico.

It is reported the obtention of monoclonal antibodies, which are capable of recognizing HSV-1 and HSV-2 viruses, by the conventional technology of cellular fusion. The monoclonal antibodies of highest positivity according to the indirect immunofluorescence technique were partially characterized. It was determined the neutralizing capacity of 2 of them (278-F7 and 70-G10), which were used to identify isolations of clinical specimens by indirect immunofluorescence. These monoclonal antibodies recognized HSV-1 up to a dilution of 1:2 560.

[Use of a dengue anti-complex monoclonal antibody in viral purification]. / Empleo de un anticuerpo monoclonal anticomplejo dengue en la purificación viral.

The purification of different serotypes of dengue virus from a monoclonal antibody which recognizes an epitope present in the four serotypes bond to a Sepharose 4B matrix activated with cyanogen bromide, is reported. Results evidence that the method employed makes quicker the process of purification with a high degree of purity.

[Characterization of monoclonal antibodies that recognize the thermolabile toxin of Vibrio cholerae]. / Caracterización de anticuerpos monoclonales que reconocen la toxina termolábil de Vibrio cholerae.

The obtention of two monoclonal antibodies which recognize a single epitope present in the subunit B of the thermolabile toxin of Vibrio cholerae and another which shows a cross-reaction with those produced by certain enteropathogenic toxins, is reported. The standardization of a solid phase indirect immunoenzymatic assay (ELISA) for the primary screening and selection of hybrids was performed; in addition, the isotype was determined.