ABSTRACT
Ozone oxidative preconditioning is a prophylactic approach, which favors the antioxidant-prooxidant balance for preservation of cell redox state by the increase of antioxidant endogenous systems in both in vivo and in vitro experimental models. Our aim is to analyze the effect of ozone oxidative preconditioning on serum TNF-¦Á levels and as a modulator of oxidative stress on hepatic tissue in entodoxic shock model (mice treated with lipopolysaccharide (LPS)). Ozone/oxygen gaseous mixture which was administered intraperitoneally (0.2, 0.4, and 1.2 mg/kg) once daily for five days before LPS (0.1 mg/kg, intraperitoneal). TNF-¦Á was measured by cytotoxicity on L-929 cells. Biochemical parameters such as thiobarbituric acid reactive substances (TBARS), enzymatic activity of catalase, glutathione peroxidase, and glutathione-S transferase were measured in hepatic tissue. One hour after LPS injection there was a significant increase in TNF-¦Á levels in mouse serum. Ozone/oxygen gaseous mixture reduced serum TNF-¦Á levels in a dose-dependent manner. Statistically significant decreases in TNF-¦Á levels after LPS injection were observed in mice pretreated with ozone intraperitoneal applications at 0.2 (78percent), 0.4 (98 percent), and 1.2 (99 percent). Also a significant increase in TBARS content was observed in the hepatic tissue of LPS-treated mice, whereas enzymatic activity of glutathion-S transferase and glutathione peroxidase was decreased. However in ozone-treated animals a significant decrease in TBARS content was appreciated as well as an increase in the activity of antioxidant enzymes. These results indicate that ozone oxidative preconditioning exerts inhibitory effects on TNF-¦Á production and on the other hand it exerts influence on the antioxidant-prooxidant balance for preservation of cell redox state by the increase of endogenous antioxidant systems(AU)
El ozono es un oxidante preacondicionamiento profil¨¢cticos, que favorece el equilibrio prooxidant antioxidantes para la preservaci¨®n de estado redox celular por el aumento de los sistemas antioxidantes end¨®genos en tanto in vivo como in vitro modelos experimentales. Nuestro objetivo es analizar el efecto oxidante del ozono preacondicionamiento en suero los niveles de TNF-¦Á y como modulador del estr¨¦s oxidativo en el tejido hep¨¢tico en entodoxic choque modelo (ratones tratados con lipopolisac¨¢rido (LPS)). Ozono y ox¨ªgeno gaseoso mezcla que se administr¨® por v¨ªa intraperitoneal (0,2, 0,4 y 1,2 mg / kg) una vez al d¨ªa durante cinco d¨ªas antes de LPS (0,1 mg / kg, intraperitoneal). TNF-¦Á se midi¨® por la citotoxicidad de las c¨¦lulas L-929. Par¨¢metros bioqu¨ªmicos, como las materias ¨¢cido tiobarbit¨²rico (TBARS), la actividad enzim¨¢tica de la catalasa, glutation peroxidasa y glutati¨®n S-transferasa se midieron en el tejido hep¨¢tico. Una hora despu¨¦s de la inyecci¨®n de LPS hubo un aumento significativo en los niveles de TNF-¦Á en suero de rat¨®n. Ozono y ox¨ªgeno gaseoso mezcla suero reducido los niveles de TNF-¦Á en una manera dosis-dependiente. Disminuciones estad¨ªsticamente significativas en los niveles de TNF-¦Á despu¨¦s de la inyecci¨®n de LPS se observaron en ratones pretratados con ozono intraperitoneal aplicaciones en 0,2 (78por ciento), 0,4 (98 por ciento) y 1,2 (99 por ciento). Tambi¨¦n un aumento significativo en el contenido de TBARS se observ¨® en el tejido hep¨¢tico de ratones tratados con LPS, mientras que la actividad enzim¨¢tica de la glutati¨®n S-transferasa y glutati¨®n peroxidasa disminuy¨®. Sin embargo, en la capa de ozono, los animales tratados una disminuci¨®n significativa en el contenido de TBARS se apreciaba, as¨ª como un aumento en la actividad de enzimas antioxidantes. Estos resultados indican que el ozono preacondicionamiento oxidativo ejerce efectos inhibidores sobre la producci¨®n de TNF..........................
