ABSTRACT
The role of lysosomal enzyme released by macrophages was examined in relation to the toxic effect caused by food yeast. Mouse peritoneal macrophages exposed to yeast in culture showed marked release of N-acetyl glucosaminidase, beta-galactosaminidase and beta-glucuronidase below the median lethal dose (LD50). LD50 was measured from the dose response curves of the cytoplasmic lactate dehydrogenase enzyme. Saccharomyces cerevisiae showed the highest LD50 followed by Kluyveromyces fragilis and Candida utilis yeast. LD50 values obtained as well as the in vitro lysosomal release by mouse peritoneal macrophages may be relevant to assess the toxic capacity of food yeast intended for human consumption.
Subject(s)
Lysosomes/enzymology , Macrophages/enzymology , Animals , Calcium/pharmacology , Candida/physiology , Cells, Cultured , Cytochalasin B/pharmacology , Hydrolases/metabolism , Lethal Dose 50 , Lysosomes/metabolism , Mice , Mice, Inbred BALB C , Saccharomyces cerevisiae/physiology , Yeast, Dried/toxicityABSTRACT
PIP: The results of a comparative study of 3 techniques for tubal occlusion are presented. These techniques were silastic ring, staple with spring, and electrofulguration. The incidence of operative complications was greater with electrofulguration, mainly tubal bleeding (18.2%). The use of the staple with rings had a very high incidence of operative difficulties (37.6%) due to the misplacement of the staple. During the postoperative period there was abdominal pain, with greater incidence in the group with the silastic ring and staple with spring (47.8%). Gynecologic problems during the late postoperative period were infrequent and unrelated to the sterilization. (author's modified)^ieng
Subject(s)
Postoperative Complications/epidemiology , Sterilization, Tubal/methods , Adult , Double-Blind Method , Electrocoagulation/adverse effects , Female , Humans , Laparoscopy/methods , Mexico , Middle Aged , Prospective Studies , Prostheses and Implants/adverse effects , Sterilization, Tubal/adverse effectsABSTRACT
Se presentan los resultados de un estudio comparativo, de tres tecnicas oclusivas tubaricas bajo control laparoscopico: anillo de silastic, grapa con resorte y electrofulguracion. Se encontro que la frecuencia de complicaciones operatorias fue mas alta con el uso de electrofulguracion, predominando los problemas de hemorragia tubarica (18.2 por ciento). El uso de grapas de resorte presento una frecuencia muy alta de dificultades operatorias (37.6 por ciento) debido a colocaciones defectuosas de las mismas.Durante el periodo postoperatorio inmediato y mediato, se presento dolor abdominal, con mayor frecuencia en el grupo de grapas de resorte y anillos de silastic, predominando en este ultimo (47.8 por ciento). Los problemas ginecologicos durante el periodo postoperatorio tardio fueron escasos y no relacionados con la esterilizacion