Pneumonia in patients with systemic lupus erythematosus: Epidemiology, microbiology and outcomes.

Background and objective Pneumonia remains the main cause of mortality in patients with systemic lupus erythematosus (SLE). The aim of the study was to establish the clinical characteristics, microbiology and risk factors for poor prognosis in patients with SLE and pneumonia. Methods We reviewed medical records of patients with SLE (American College of Rheumatology criteria) and pneumonia who attended the emergency room in a single tertiary care center (January 2010-March 2015). We collected demographics, treatment and disease activity (SLEDAI-2K) data. Severity scales of pneumonia (CURB-65 (acronym for risk factors measured: confusion, urea nitrogen, respiratory rate, blood pressure, 65 years of age and older) and Pneumonia Severity Index (PSI)) were obtained. A negative composite outcome was defined as need for mechanical ventilation, septic shock or death secondary to pneumonia up to 30 days after discharge. We conducted a univariate and multivariable analysis. Results We studied 158 patients (76% women) with 187 episodes of pneumonia. There were no differences in age, SLE duration, SLE activity, treatment or comorbidities between patients with negative composite outcome vs the other group. In 53 episodes, patients presented with a negative composite outcome. Of these, 46 (24.6%) required intubation, 13 (7%) developed shock and 12 (6.4%) died. The most common bacteria isolated was S. aureus, and we observed a high percentage of nonhabitual microorganisms. Fifteen percent of patients who presented with a negative outcome had low values on CURB-65 and PSI scales. Conclusion Patients with SLE and pneumonia have a high risk of complications and present with a high percentage of nonhabitual microorganisms. Severity scales for pneumonia can misclassify as low risk SLE patients with poor prognosis.

Ant Foraging As an Indicator of Tropical Dry Forest Restoration.

Variation in foraging behavior may indicate differences in food availability and allow assessment of restoration actions. Ants are prominent bioindicators used in assessing ecological responses to disturbance. However, behavioral data have been poorly incorporated as an index. The foraging performance of red harvester ants was quantified in order to evaluate the success of a restoration ecology experiment in the tropical dry forest of Sierra de Huautla, Morelos, in central Mexico. Foraging performance by granivorous, Pogonomyrmex barbatus, ants was diminished after 6 and 8 years of cattle grazing and wood harvest were excluded as part of a restoration experiment in a highly degraded biome. Despite investing more time in foraging, ant colonies in exclusion plots showed lower foraging success and acquired less seed biomass than colonies in control plots. In line with the predictions of optimal foraging theory, in restored plots where ant foraging performance was poor, ants harvested a higher diversity of seeds. Reduced foraging success and increased harvest of non-preferred foods in exclusion plots were likely due to the growth of herbaceous vegetation, which impedes travel by foragers. Moreover, by 8 years of exclusion, 37% of nests in exclusion plots had disappeared compared to 0% of nests in control plots. Ants' foraging success and behavior were sensitive to changes in habitat quality due to the plant successional process triggered by a restoration intervention. This study spotlights on the utility of animal foraging behavior in the evaluation of habitat restoration programs.

Effect of recombinant prophenin 2 on the integrity and viability of Trichomonas vaginalis.

Trichomonas vaginalis is the causal agent of trichomoniasis, which is associated with preterm child delivery, low birth weight, and an increased risk of infection by human papilloma virus and human immunodeficiency virus following exposure. Several reports have established increasing numbers of trichomoniasis cases resistant to metronidazole, the agent used for treatment, and it is therefore important to identify new therapeutic alternatives. Previously, our group reported the effect of tritrpticin, a synthetic peptide derived from porcine prophenin, on T. vaginalis; however, the hemolytic activity of this small peptide complicates its possible use as a therapeutic agent. In this study, we report that the propeptide and the processed peptide of prophenin 2 (cleaved with hydroxylamine) affected the integrity and growth of T. vaginalis and that pro-prophenin 2 displays some resistance to proteolysis by T. vaginalis proteinases at 1 h. Its effect on T. vaginalis as well as its low hemolytic activity and short-time stability to parasite proteinases makes prophenin 2 an interesting candidate for synergistic or alternative treatment against T. vaginalis.