ABSTRACT
Leaf share, plant age and growth season are often overlooked as modifiers of the biomass quality in energy crops. The current work studied the effect of the given factors on the biomass yield and the biomass quality in Elephant grass CT115, intended for bioethanol production, in Veracruz, Mexico. Two seasons per year, 5 months each, were tracked on a 2-weeks basis. The climate is warm wet with summer rains, 1,142 mm of annual rainfall, and 26 °C monthly temperature. From day 56 of the wet season or from day 84 of the dry season, stems accumulated 12 or 6 Mg ha-1, respectively, while green leaves increased only 1 Mg. Higher biomass quality was recorded for the leaf fraction, or for the wet season regrowth. For instance, lignin contained in stems meant twice that of leaves, whereas stems recorded 20% less lignin in the wet season as compared to the dry season. Despite holocellulose being similar between fractions or seasons, hemicellulose and cellulose showed inverse correlation, while lignin and cellulose contents were directly correlated in stems. Increasing the annual harvest of green leaves will improve biomass quality, which is known to increase biodegradability and might improve the annual ethanol yield.
ABSTRACT
The Pelibuey sheep has adaptability to climatic variations, resistance to parasites, and good maternal ability, whereas some ewes present multiple births, which increases the litter size in farm sheep. The litter size in some wool sheep breeds is associated with the presence of mutations, mainly in the family of the transforming growth factor ß (TGF-ß) genes. To explore genetic mechanisms underlying the variation in litter size, we conducted a genome-wide association study in two groups of Pelibuey sheep (multiparous sheep with two lambs per birth vs. uniparous sheep with a single lamb at birth) using the OvineSNP50 BeadChip. We identified a total of 57 putative SNPs markers (p < 3.0 × 10-3, Bonferroni correction). The candidate genes that may be associated with litter size in Pelibuey sheep are CLSTN2, MTMR2, DLG1, CGA, ABCG5, TRPM6, and HTR1E. Genomic regions were also identified that contain three quantitative trait loci (QTLs) for aseasonal reproduction (ASREP), milk yield (MY), and body weight (BW). These results allowed us to identify SNPs associated with genes that could be involved in the reproductive process related to prolificacy.
ABSTRACT
The Pelibuey sheep (Ovis aries) is an indigenous breed distributed in the tropical regions of Mexico. The prolificacy of this sheep is on average from 1 to 1.5 lambs, being an important breeding characteristic that owners seek to increase with the purpose of economic improvements. New-generation RNA sequencing technology has been used to identify the genes that are expressed in the ovarian tissue of sheep that have two or more lambs per parturition, as well as to elucidate the metabolic pathways that are affected by the expression of these genes, with the purpose of better understanding the prolificacy in the sheep. In the present study, the transcriptional expression of multiparous and uniparous sheep was compared using RNA sequencing. Multiparous (M group) and uniparous (U group) sheep that had a genealogical record for three generations (M, n = 5 and U, n = 5) were selected. RNA was extracted from ovarian tissue and subsequently used to prepare the libraries that were sequenced using the Illumina NextSeq500 platform. A total of 31,575 genes were detected from the transcriptomic analysis of which 4908 were significantly expressed (p-value ≤ 0.001) in the ovary of sheep. Subsequently, a second filter was carried out to evaluate the false discovery rate (FDR) and select those genes with p-values ≤ 0.05 and values of expression ≥ 1 (log2), obtaining 354 differential expressed genes (DEG): 120 genes up-regulated and 234 genes down-regulated in the group M with respect to the group U. Through Gene Ontology (GO) and metabolic analysis, we obtained information on the function of differentially expressed genes, and its importance in the reproduction of multiparous sheep. This result suggest that genes identified in the present study participate in the development of the final stages of follicles.
