Collective migration of an epithelial monolayer in response to a model wound.

Using an original microfabrication-based technique, we experimentally study situations in which a virgin surface is presented to a confluent epithelium with no damage made to the cells. Although inspired by wound-healing experiments, the situation is markedly different from classical scratch wounding because it focuses on the influence of the free surface and uncouples it from the other possible contributions such as cell damage and/or permeabilization. Dealing with Madin-Darby canine kidney cells on various surfaces, we found that a sudden release of the available surface is sufficient to trigger collective motility. This migration is independent of the proliferation of the cells that mainly takes place on the fraction of the surface initially covered. We find that this motility is characterized by a duality between collective and individual behaviors. On the one hand, the velocity fields within the monolayer are very long range and involve many cells in a coordinated way. On the other hand, we have identified very active "leader cells" that precede a small cohort and destabilize the border by a fingering instability. The sides of the fingers reveal a pluricellular actin "belt" that may be at the origin of a mechanical signaling between the leader and the followers. Experiments performed with autocrine cells constitutively expressing hepatocyte growth factor (HGF) or in the presence of exogenous HGF show a higher average velocity of the border and no leader.

Rayleigh-Mie Doppler wind lidar for atmospheric measurements. I. Instrumental setup, validation, and first climatological results.

Since 1989 Service d'Aéronomie du Centre National de la Recherche Scientifique has used an incoherent Doppler lidar technique for wind measurements in the atmosphere. A new-generation Rayleigh-Mie Doppler lidar has been designed and is currently operated at the Observatoire de Haute Provence (France). We give a detailed description of this instrument and highlight two important upgrades leading to quasi-simultaneous and absolute measurements of the wind from approximately 8 to 50 km altitude. The possible sources of error are identified and quantified in terms of accuracy in the wind determination. Experimental results are given in detail, and a validation of the measurements is performed with the help of ancillary data. A first climatological description of the mean wind is briefly reported.

Rayleigh-Mie Doppler wind lidar for atmospheric measurements. II. Mie scattering effect, theory, and calibration.

A theoretical and experimental study is conducted for the direct-detection Doppler Lidar developed by the Service d'Aéronomie du Centre National de la Recherche Scientifique. Thanks to a specific design, the double-edge technique that applies primarily to Rayleigh scattering can also be employed in presence of aerosols backscatter. We focus on a careful estimate of the particle-induced error on the wind measurements. With a theoretical model for the Fabry-Perot interferometer and two sets of calibration measurements, the true spectral properties of the interferometer and the calibration curves are recovered. Furthermore, the particle-induced error is estimated for varying values of the scattering ratio at 532 nm. When applied to real atmospheric signals, this error is shown to be negligible. A comparison between ancillary data and the wind and backscatter ratio as retrieved from the Doppler lidar signals confirms our estimate.