ABSTRACT
OBJECTIVES: The effect of different media and composition on the in vitro activity of posaconazole, caspofungin and voriconazole against 59 zygomycetes species was determined. METHODS: The media tested were RPMI 1640 medium with and without 2% glucose, antibiotic medium 3 (AM3) with and without 2% glucose, and high resolution (HR) medium. RESULTS: Posaconazole was significantly more active than caspofungin and voriconazole, both in RPMI 1640 medium with 2% glucose and in HR medium. Adding glucose improved the determination of end points, but had only minor influence on the MICs. MICs evaluated in AM3 were lower than in RPMI 1640 medium or HR medium. CONCLUSIONS: The in vivo effect of posaconazole in zygomycosis needs further evaluation.
Subject(s)
Antifungal Agents/pharmacology , Mucorales/drug effects , Peptides, Cyclic/pharmacology , Pyrimidines/pharmacology , Triazoles/pharmacology , Absidia/drug effects , Absidia/growth & development , Caspofungin , Culture Media , Cunninghamella/drug effects , Cunninghamella/growth & development , Echinocandins , Lipopeptides , Microbial Sensitivity Tests , Mucor/drug effects , Mucor/growth & development , Mucorales/growth & development , Rhizopus/drug effects , Rhizopus/growth & development , VoriconazoleABSTRACT
We evaluated the effect of the combination of micafungin and polymorphonuclear leukocytes (PMN) against hyphae of Candida albicans and Candida dubliniensis. Micafungin enhanced the PMN oxidative burst dose dependently. The combination was synergistic (C. albicans) or additive (C. dubliniensis); when PMN were pretreated with granulocyte-macrophage colony-stimulating factor, the combination was more effective.
