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1.
Thromb Haemost ; 89(6): 1004-15, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12783113

ABSTRACT

Platelet activation is reported to correlate with acute coronary syndromes. A platelet analysis method on the ADVIA 120 Hematology System provides rapid analysis of platelet density, reported as mean platelet component (MPC) concentration, utilizes routine hematology specimens, requires no pre-treatment, and thirty seconds to generate results. Sub-populations of platelets separated by density gradients showed excellent correlation with the ADVIA 120 MPC parameter (r = 0.997). Platelet activation induced by thrombin treatment resulted in a shift of platelets into the lowest density fraction (d

Subject(s)
Cell Degranulation , Flow Cytometry/methods , Platelet Activation , Automation , Calibration , Flow Cytometry/instrumentation , Humans , P-Selectin/analysis , Platelet Function Tests/instrumentation , Platelet Function Tests/methods , Thrombin
2.
Vet Clin Pathol ; 27(1): 2-9, 1998.
Article in English | MEDLINE | ID: mdl-12075552

ABSTRACT

A new whole-blood flow cytometric method has been developed for counting and sizing platelets in samples from cats, a species in which platelet and red blood cell sizes overlap significantly. The method is a modified version of the two-angle laser light scattering technology used by Bayer H*System hematology analyzers. The new method provided accurate platelet counts and mean platelet volumes (MPV, fl) for cats. The method also measured mean platelet component concentration (MPC, g/dl), a parameter which was shown to be sensitive to platelet activation state, and which decreased in value as activation progressed.

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