Long-term and resegmentation precision of quantitative cartilage MR imaging (qMRI).

OBJECTIVE: Follow up of osteoarthritis (OA) and evaluation of structure modifying OA drugs require longitudinal data on cartilage structure. The aim of this study was to analyse the long term and resegmentation precision of quantitative cartilage analysis with magnetic resonance imaging (qMRI) in vivo, and to relate precision errors to the estimated cartilage loss in OA. METHOD: Sagittal MR images of the knee were obtained in 14 individuals, four datasets being acquired in a first imaging session. In 12 subjects, two further datasets were acquired over the next months. Image analysis was performed in the same session for image data obtained under short-term and long-term imaging conditions, and in three different sessions (months apart) for the first data set (resegmentation precision). RESULTS: Long-term precision errors ranged from 1.4% (total knee) to 3.9% (total femur) for cartilage volume and thickness and were only marginally higher than those under short term conditions. In the medial tibia, the error was 84 mm(3) compared with an estimated loss of >1,200 mm(3) in varus OA. Precision errors for resegmentation were somewhat higher, but considerably smaller than the intersubject variability. CONCLUSIONS: Scanner drift and changes in imaging or patient conditions appear not to represent a critical problem in quantitative cartilage analysis with magnetic resonance imaging (qMRI). In longitudinal studies, image analysis of sequential data should be performed within the same post-processing session. Under these conditions, qMRI promises to be a very powerful method to assess structural change of cartilage in OA.

Optimization and validation of a rapid high-resolution T1-w 3D FLASH water excitation MRI sequence for the quantitative assessment of articular cartilage volume and thickness.

In view of follow up, survey and development of therapeutic strategies for osteoarthritis where cartilage deterioration plays an important role, a non invasive, reliable and quantitative assessment of the articular cartilage is desirable. The currently available high resolution T(1)-weighted (T1-w) 3D FLASH pulse sequences with frequency selective fat suppression are very time consuming. We have 1) optimized a high resolution T1-w 3D FLASH water excitation (WE) sequence for short acquisition time and cartilage visualization, and 2) validated this sequence for cartilage volume and thickness quantification. The spectral fat presaturation was replaced by selective water excitation. The flip angle of the WE sequence was optimized for the contrast to noise (C/N(cart)) ratio of cartilage. Sagittal datasets (voxel size: 0.31 x 0.31 x 2 mm(3)) of the knees of nine healthy volunteers were acquired both, with the 3D FLASH WE (17.2/6.6/30 degrees ) sequence (WE) and a previously validated 3D FLASH fat saturated (42/11/30 degrees ) sequence (FS). For validation of the WE sequence, cartilage volume, mean and maximal cartilage thickness of the two sequences were compared. Reproducibility was assessed by calculating the coefficient of variation (COV %) of 4 consecutive WE data sets in the volunteers. The acquisition time was reduced from 16'30" (FS) down to 7'14" for the WE sequence. Image contrast and visualization of the cartilage was very similar, but delineation of the basal layer of the cartilage was slightly improved with the WE sequence. A flip angle of 30 degrees provided the best C/N(cart) ratios (WE). Reproducibility (COV) was between 1.9 and 5.9%. Cartilage volume and thickness agreed within 4% between FS and WE sequence. The WE sequence allows for rapid, valid and reproducible quantification of articular cartilage volume and thickness, prerequisites for follow-up examinations. The reduced acquisition time (50% of FS) enables routine clinical application and thus may contribute to a broader assessment of osteoarthritis.

[Precision MRI-based joint surface and cartilage density analysis of the knee joint using rapid water-excitation sequence and semi-automatic segmentation algorithm]. / Präzision MRT-basierter Gelenkflächen- und Knorpeldickenanalysen im Kniegelenk bei Verwendung einer schnellen Wasseranregungs-Sequenz und eines semiautomatischen Segmentierungs-Algorithmus.

The aim of this study was to analyse the precision of three-dimensional joint surface and cartilage thickness measurements in the knee, using a fast, high-resolution water-excitation sequence and a semiautomated segmentation algorithm. The knee joint of 8 healthy volunteers, aged 22 to 29 years, were examined at a resolution of 1.5 mm x 0.31 mm x 0.31 mm, with four sagittal data sets being acquired after repositioning the joint. After semiautomated segmentation with a B-spline Snake algorithm and 3D reconstruction of the patellar, femoral and tibial cartilages, the joint surface areas (triangulation), cartilage volume, and mean and maximum thickness (Euclidean distance transformation) were analysed, independently of the orientation of the sections. The precision (CV%) for the surface areas was 2.1 to 6.6%. The mean cartilage thickness and cartilage volume showed coefficients of 1.9 to 3.5% (except for the femoral condyles), the value for the medial femoral condyle being 9.1%, and for the lateral condyle 6.5%. For maximum thickness, coefficients of between 2.6 and 5.9% were found. In the present study we investigate for the first time the precision of MRI-based joint surface area measurements in the knee, and of cartilage thickness analyses in the femur. Using a selective water-excitation sequence, the acquisition time can be reduced by more than 50%. The poorer precision in the femoral condyles can be attributed to partial volume effects that occur at the edges of the joint surfaces with a sagittal image protocol. Since MRI is non-invasive, it is highly suitable for examination of healthy subjects (generation of individual finite element models, analysis of functional adaptation to mechanical stimulation, measurement of cartilage deformation in vivo) and as a diagnostic tool for follow-up, indication for therapy, and objective evaluation of new therapeutic agents in osteoarthritis.