ABSTRACT
The isoenzymes of lactate dehydrogenase (EC 1.1.1.27) were separated with excellent resolution in less than 1 h by high-pressure anion-exchange chromatography. These isoenzymes were determined in some tissue extracts and high-activity serum samples by this technique.
Subject(s)
L-Lactate Dehydrogenase/analysis , Chromatography, High Pressure Liquid/instrumentation , Chromatography, High Pressure Liquid/methods , Humans , Isoenzymes , L-Lactate Dehydrogenase/blood , L-Lactate Dehydrogenase/isolation & purification , Myocardium/enzymologySubject(s)
Cholesterol/analysis , Sulfuric Acids , Colorimetry , Evaluation Studies as Topic , Kinetics , Mathematics , Methods , Time FactorsABSTRACT
We have applied a comprehensive theory of photometric errors to the design and evaluation of an inexpensive stabilized photometer. The photometer is described in terms of a group of modules, the characteristics of which are described in terms of their effect on specific error coefficients. Procedures described in this paper show how the principles described earlier [Clin, Chem. 20, 1028 (1974)] can be used to optimize the design of new instrumentation. Chemical data are included to verify the agreement between predicted and experimental results.