Revisions to the derivation of the Australian and New Zealand guidelines for toxicants in fresh and marine waters.

The Australian and New Zealand Guidelines for Fresh and Marine Water Quality are a key document in the Australian National Water Quality Management Strategy. These guidelines released in 2000 are currently being reviewed and updated. The revision is being co-ordinated by the Australian Department of Sustainability, Environment, Water, Population and Communities, while technical matters are dealt with by a series of Working Groups. The revision will be evolutionary in nature reflecting the latest scientific developments and a range of stakeholder desires. Key changes will be: increasing the types and sources of data that can be used; working collaboratively with industry to permit the use of commercial-in-confidence data; increasing the minimum data requirements; including a measure of the uncertainty of the trigger value; improving the software used to calculate trigger values; increasing the rigour of site-specific trigger values; improving the method for assessing the reliability of the trigger values; and providing guidance of measures of toxicity and toxicological endpoints that may, in the near future, be appropriate for trigger value derivation. These changes will markedly improve the number and quality of the trigger values that can be derived and will increase end-users' ability to understand and implement the guidelines in a scientifically rigorous manner.

Controlling the invasive diatom Didymosphenia geminata: an ecotoxicity assessment of four potential biocides.

In 2004, an invasive mat-forming freshwater diatom, Didymosphenia geminata (didymo), was found in New Zealand causing concern with regard to potential consequences for local freshwater ecosystems. A four-stage research program was initiated to identify methods to control D. geminata. This article reports the results of Stage 2, in which four potential control compounds [Gemex™ (a chelated copper formulation), EDTA, Hydrothol®191, and Organic Interceptor™ (a pine oil formulation)] selected in Stage 1 were evaluated for their biocidal efficacy on D. geminata and effects on non-target organisms using both artificial stream and laboratory trials. Artificial stream trials evaluated the mortality rates of D. geminata and fishes to three concentrations of the four biocides, whereas laboratory toxicity trials tested the response of green alga and cladocera to a range of biocide concentrations and exposure times. In artificial stream trials, Gemex and Organic Interceptor were the most effective biocides against D. geminata for a number of measured indices; however, exposure of fishes to Organic Interceptor resulted in high mortality rates. Laboratory toxicity testing indicated that Gemex might negatively affect sensitive stream invertebrates, based on the cladoceran sensitivity at the proposed river control dose. A decision support matrix evaluated the four biocides based on nine criteria stipulated by river stakeholders (effectiveness, non-target species impacts, stalk removal, degradation profile, risks to health and safety, ease of application, neutralization potential, cost, and local regulatory requirements) and Gemex was identified as the product warranting further refinement prior to an in-river trial.

Genotype-dependent recovery from acute exposure to heavy metal contamination in the freshwater clam Sphaerium novaezelandiae.

The ability to recover from environmental perturbations is essential for the sustainability of ecological systems. Variation in the ability of individual organisms to recover from stressors influences overall resilience at higher levels of biological organisation. Such variation is likely to be genetically based. To investigate this hypothesis we examined the genetic basis of both resistance to and recovery from zinc, a common stormwater contaminant, in the New Zealand freshwater clam Sphaerium novaezelandiae. We undertook a 4-day toxicity test using zinc exposure concentrations ranging from 0.31 to 5.00 mg/L. These concentrations are consistent with levels recorded in urban streams during the first flush of storms. As our response measures we recorded mortality at the end of the 4-day period, as well as reburial rate (time to rebury in sediment) following the 4-day exposure ("exposure") and then again following a 24h period of recovery ("recovery"). Genotypic composition was determined using allozyme electrophoresis, focusing on the enzyme Pgm (phosphoglucomutase). Overall, a significant effect on mortality was observed, with an average value of 78.6% (+/-7.9) at 5.00 mg/L zinc, compared with only 3.8% (+/-3.8) mortality at 0.31 mg/L zinc. An inhibition concentration (IC(50)) of 1.16 mg/L was recorded, when considered regardless of genotypes. There was no significant genotype-specific differences in mortality. There was a significant difference in reburial rates across all genotypes at the end of the exposure period with an average reburial time of 83.0+/-3.6 min at 5.00 mg/L (22.8+/-2.9 min at 0.31 mg/L). There was a near-significant (p=0.058) difference in time taken to rebury when comparing between genotypes at the "exposure" stage for any concentration. Significant differences in reburial rates across all genotypes were also observed following 24h recovery. When individual genotypes were compared at this stage, genotype 33 reburied on average significantly faster (24.0+/-4.5 min) than other genotypes at the highest exposure concentration and was also significantly faster than genotype 44 at 1.25mg/L. Studies investigating the genetic basis to recovery from stressors at an individual level are limited. This study has shown that populations of organisms display genetically-based variation in their ability to recover from zinc exposure in the laboratory and that such variation is linked to a physiological trait (reburial). The potential effects on other life history traits (e.g. feeding), possible physiological trade-offs and the implications for such variation on ecosystem resilience requires further investigation.

Chronic toxicity of ammonia to the freshwater bivalve Sphaerium novaezelandiae.

The chronic toxicity of total ammonium and unionized ammonia (NH3) to the native New Zealand freshwater fingernail clam Sphaerium novaezelandiae was assessed in soft water under laboratory conditions. Control survival after 60 days was high (93%) and concentration-response relationships showed the sensitivity of S. novaezelandiae survival was markedly greater to both total and unionized ammonia (6.4x and 4.6x) after 60 days compared with the 30-day exposure at 20 degrees C. Chronic mortality and number moribund (inability to rebury) showed similar sensitivities, but reproduction was a more sensitive endpoint based on a concentration-response analysis. The survival LC50 values for total and unionized ammonia were 3.8 mg (N)/L (pH 7.5) and 0.037 mg (NH3-N)/L, and reproductive values 0.80 mg (N)/L (pH 7.5) and 0.013 mg (NH3-N)/L at 60 days. No observed effect concentration (NOEC) values for both survival and reproduction were 0.97 mg (N)/L and 0. 011 mg (NH3-N)/L, and the lowest observed effect concentration (LOEC) values were 5.4 mg (N)/L and 0.046 mg (NH3-N)/L for survival after 60 days, giving a calculated threshold effect concentration (TEC) of 2.3 mg (N)/L and 0.022 mg (NH3-N)/L. Comparison of the S. novaezelandiae chronic ammonia sensitivity data with the US EPA criteria showed the survival and reproduction TEC values for total ammonia were 1.9x higher than the chronic criterion, and the lethality value 1.4x above the unionized ammonia criterion. The findings suggest that use of the US EPA criteria would provide minimal protection for S. novaezelandiae for chronic ammonia exposure, and that development of site-specific criteria, covering a wide range of environmental conditions, may be required to adequately protect all life stages of this species.

Quantitative addition of dissolved oxygen to in situ benthic chamber systems by use of catalase and hydrogen peroxide.

A methodology for reoxygenation of in situ benthic chamber systems by enzymatic catalysis of hydrogen peroxide with catalase was developed. For a 10-liter benthic chamber, the injection of 1 ml of catalase suspension (26,000 U ml) followed by 10 ml of 0.5 M hydrogen peroxide solution resulted in complete reoxygenation within 2.5 min at 25 degrees C.