ABSTRACT
In this research, the protein participation that occurs with the flocculation and sedimentation phenomena caused by adding enzymes to solid particles derived from rice washing drainage and the electrostatic properties of the solid particle surfaces was examined. As seen in the results, adding protease to rice washing drainage decreased the protein content, strengthened the negative surface electron charge, and decreased the isoelectric point (IEP) value of the solid particles. In addition, about 25% of the proteins in the rice washing drainage still remained after enzyme treatment. Moreover, due to the enzyme treatment, specific and gradual degradations of 10, 13, and 16kDa of prolamin and 20-22kDa of gluterin basic subunit, both categorized in the basic protein group, were observed in an SDS-PAGE analysis. These results suggest that the flocculation and sedimentation of solid particles in rice washing drainage occur as a result of the formation of the IEP-dependent strengthening of the negative surface electron charge on the solid particle surface.
Subject(s)
Enzymes/chemistry , Food Handling/methods , Oryza/chemistry , Plant Proteins/analysis , Electrophoresis, Polyacrylamide Gel , Flocculation , Isoelectric Point , Particle Size , Plant Proteins/chemistry , Prolamins/chemistry , Surface PropertiesABSTRACT
Bioethanol production from rice washing drainage and rice bran derived from a rinse-free process of rice manufacturing was established using the combinations of an enzymatic procedure and an ultra-sonic procedure of rice washing drainage for a sample pre-treatment. The maximum ethanol concentration was attained at 6.2% (v/v).
