Microbiological, sensory, and chemical properties of high-quality tempeh made with instant Mosaccha tempeh inoculum powder.

The combination of Saccharomyces cerevisiae and Rhizopus oligosporus liquid inoculum has been successfully used to ferment soybeans into tempeh that contains ß-glucan. However, using the liquid inoculum of these two microbes as a starter is impractical; so, developing an instant tempeh dry inoculum in powdered form, called the Mosaccha inoculum powder, for ease of use is necessary. This study aimed to determine the best concentration of instant Mosaccha inoculum powder to produce high-quality Mosaccha tempeh. The study used a Complete Randomized Block Design with seven different levels of instant Mosaccha inoculum powder percentage, ranging from 0.3% to 1.8% (w/w). A commercial tempeh inoculum, RAPRIMA, amounting to 0.2%, was used as control. Then, the microbiological (total mold and total yeast) and sensory (color, aroma, texture, and taste) properties were evaluated. The data obtained was analyzed statistically using analysis of variance (ANOVA) and Honestly Significant Difference (HSD) tests at the 5% level. The results showed that the percentage of instant Mosaccha inoculum powder significantly affected the microbiological and sensory properties of Mosaccha tempeh. A concentration of Mosaccha inoculum powder between 0.6% to 1.8% could produce good quality Mosaccha tempeh, but the best Mosaccha tempeh was produced with 1.5% instant Mosaccha inoculum powder, which met the Indonesian National Standards (SNI) 3144:2015, had a very favorable taste, and contained 0.49% ß-glucan. Therefore, Mosaccha inoculum in powdered form can be developed and used as a starter in making high-quality tempeh that contains ß-glucan.

Saponin Extracts Utilization as Dietary Additive in Ruminant Nutrition: A Meta-Analysis of In Vivo Studies.

The present meta-analysis aimed to determine the underlying effects of different saponins extracted from different sources on the production performance, milk yield, digestibility, rumen fermentation, blood metabolites, and nitrogen utilization of ruminants. A total of 26 papers comprising 66 in vivo studies (148 data points of dietary treatments) were evaluated in the present study. The databases were statistically analyzed using the mixed model procedure of SAS, where experiments considered random effects and tannin-related factors were treated as fixed effects. Statistical procedures were then continued in comparing different sources of saponin extract through Mixed Model analysis, where experiments were also random factors and sources of saponin extract were fixed factors. The evidence revealed in the present meta-analysis that saponin supplementation of up to 40 g/kg DM appears to have no detrimental impact on feed intake across ruminant types, suggesting that it does not significantly affect diet palatability. However, the results indicated that there are species-specific responses to saponin supplementation, particularly in relation to palatability and nutrient absorption efficiency, with larger ruminants being better able to tolerate the bitterness induced by saponin extracts. Furthermore, the study found that saponin extracts can influence nutrient digestibility and rumen fermentation dynamics, with different effects observed in large and small ruminants. While some saponin extracts can enhance average daily weight gain and milk yield, others can have adverse effects, highlighting the importance of considering both saponin sources and animal physiological condition when developing nutritional strategies. Additionally, optimization of ruminant production by utilizing saponin extracts is necessary to avoid negative health implications, such as increased blood creatinine levels. Different saponin extracts utilization in ruminant nutrition and environmental management, have a distinct understanding associated to their various bioactive properties. However, among the saponin sources, saponin extracted from Quilaja saponaria is more likely to improve large ruminant production performance while maintaining ruminant health and metabolism, but negatively affect small ruminants. Further research is needed to unravel the intricate effects of different saponin sources on ruminant health and productivity, emphasizing the importance of tailored dietary strategies that consider the unique physiological and metabolic characteristics of the target livestock.

Crystal structures and photoluminescence characteristics of cesium lead bromide perovskite nanoplatelets depending on the antisolvent and ligand used in their syntheses.

Cesium lead bromide (CsPbBr3) nanocrystals (NCs) with nanoplatelet shapes and different crystal structures were synthesized via the ligand-assisted reprecipitation (LARP) method using different pairs of ligands and antisolvents, namely oleic acid (OA) or linoleic acid (LA) as the ligand and toluene or chloroform as the antisolvent. The XRD data revealed that the obtained CsPbBr3 NCs have different crystal structures, namely orthorhombic, tetragonal, and cubic, depending on the ligand and antisolvent pair, which exhibited significantly different photoluminescence (PL) characteristics. From the XPS data, these CsPbBr3 nanoplatelets showed two doublet peaks of the Br-3d orbital at different binding energies, representing two different chemical environments of the Br bonds. The doublet peak apparent at a higher binding energy was associated with the Br chemical states at the crystal surface, which appeared because of the distorted crystal structure resulting from the interaction of the solvent and ligand with Br ions. The PL emission consists of three luminescence centers: a PL band peaked at 520 nm (A band), a PL band peaked at 540 nm (B band), and a PL band tail, which can be discussed in terms of exciton models. Stable and intense luminescence was observed in CsPbBr3 nanoplatelets synthesized using a pair of toluene antisolvent and LA ligand, namely CsPbBr3#(Tl/LA). The orthorhombic crystal structure and distorted crystal surface in this sample may lead to confinement of the photogenerated small exciton-polaron and weak phonon interactions, which effectively hinder exciton dissociation, particularly at the crystal surface, resulting in intense PL. The results of this study may provide additional important insights into the role of the antisolvent and ligand in the formation of CsPbBr3 NCs and the exciton behavior in their PL characteristics, which may also be found in other types of halide perovskites.

The Effects of 16-HETE Enantiomers on Hypertrophic Markers in Human Fetal Ventricular Cardiomyocytes, RL-14 Cells.

BACKGROUND: Cytochrome P450 (CYP) metabolizes arachidonic acid to produce bioactive metabolites such as EETs and HETEs: mid-chain, subterminal, and terminal HETEs. Recent studies have revealed the role of CYP1B1 and its associated cardiotoxic mid-chain HETE metabolites in developing cardiac hypertrophy and heart failure. Subterminal HETEs have also been involved in various physiological and pathophysiological processes; however, their role in cardiac hypertrophy has not been fully defined. OBJECTIVE: The objective of the current study is to determine the possible effect of subterminal HETEs, R and S enantiomers of 16-HETE, on CYP1B1 expression in vitro using human cardiomyocytes RL-14 cells. METHODS: In the study, RL14 cell line was treated with vehicle and either of the 16-HETE enantiomers for 24 h. Subsequently, the following markers were assessed: cell viability, cellular size, hypertrophic markers, CYP1B1 gene expression (at mRNA, protein, and activity levels), luciferase activity, and CYP1B1 mRNA and protein half-lives. RESULTS: The results of the study showed that 16-HETE enantiomers significantly increased hypertrophic markers and upregulated CYP1B1 mRNA and protein expressions in RL-14 cell line. The upregulation of CYP1B1 by 16-HETE enantiomers occurs via a transcriptional mechanism as evidenced by transcriptional induction and luciferase reporter assay. Furthermore, neither post-transcriptional nor post-translational modification was involved in such modulation since there was no change in CYP1B1 mRNA and protein stabilities upon treatment with 16-HETE enantiomers. CONCLUSION: The current study provides the first evidence that 16R-HETE and 16S-HETE increase CYP1B1 gene expression through a transcriptional mechanism.

Metagenomic analysis of mycobiome in wild and captivity Sumatran orangutans (Pongo abelii).

Objective: This study analyzes the mycobiome in wild and captive Sumatran orangutans. Materials and Methods: Nine orangutan feces samples from the wild and nine from captivity were divided into three repeats from 11- to 15-year-olds in good health. The Illumina platform for analysis of ITS bioinformatics was used according to the Qiime2 and CCMetagen approaches. Results: Wild Sumatran orangutans include 53% Ascomycota, 38% uncultured fungi, and 4% Basidiomycota. Orangutans in captivity are 57% Ascomycota, 26% uncultured fungi, and 2% Basidiomycota. Based on genus level, uncultured Neurospora (31%), Penicillium (10%), Aspergillus (3%), Fusarium (3%), Candida (2%), Cutaneotrichosporon (2%), and Limonomyces (2%) are found in wild orangutans. The most prevalent genus among captivity orangutans is Aspergillus (32%), followed by fungal sp. (11%), Lasiodiplodia (18%), Devriesia (2%), and Sordariomycetes (2%). According to the Chao1 diversity index and Shannon and Simpson, there was no significant difference between wild and captive Sumatran orangutans. Conclusion: Neurospora is unique to wild Sumatran orangutans, although Aspergillus predominates in captive orangutans. We hypothesize that the gut mycobiome of wild orangutans will resemble that of orangutans in captivity. The excellent range of food sources in the forest does not result in the prevalence of fungi in the typical gut microbiome.

16R-HETE and 16S-HETE alter human cytochrome P450 1B1 enzyme activity probably through an allosteric mechanism.

Cytochrome P450 1B1 (CYP1B1) has been widely associated with the development of cardiac pathologies due to its ability to produce cardiotoxic metabolites like midchain hydroxyeicosatetraenoic acids (HETEs) from arachidonic acid (AA) through an allylic oxidation reaction. 16-HETE is a subterminal HETE that is also produced by CYP-mediated AA metabolism. 19-HETE is another subterminal HETE that was found to inhibit CYP1B1 activity, lower midchain HETEs, and have cardioprotective effects. However, the effect of 16-HETE enantiomers on CYP1B1 has not yet been investigated. We hypothesized that 16(R/S)-HETE could alter the activity of CYP1B1 and other CYP enzymes. Therefore, this study was carried out to investigate the modulatory effect of 16-HETE enantiomers on CYP1B1 enzyme activity, and to examine the mechanisms by which they exert these modulatory effects. To investigate whether these effects are specific to CYP1B1, we also investigated 16-HETE modulatory effects on CYP1A2. Our results showed that 16-HETE enantiomers significantly increased CYP1B1 activity in RL-14 cells, recombinant human CYP1B1, and human liver microsomes, as seen by the significant increase in 7-ethoxyresorufin deethylation rate. On the contrary, 16-HETE enantiomers significantly inhibited CYP1A2 catalytic activity mediated by the recombinant human CYP1A2 and human liver microsomes. 16R-HETE showed stronger effects than 16S-HETE. The sigmoidal binding mode of the enzyme kinetics data demonstrated that CYP1B1 activation and CYP1A2 inhibition occurred through allosteric regulation. In conclusion, our study provides the first evidence that 16R-HETE and 16S-HETE increase CYP1B1 catalytic activity through an allosteric mechanism.

Dual plasmonic modes in the visible light region in rectangular wave-shaped surface relief plasmonic gratings.

Rectangular wave-shaped surface-relief plasmonic gratings (RSR-PGs) have been fabricated from a hybrid polymer by employing a simple nanoimprint photocuring lithography technique using a silicon template, followed by gold nanolayer metallization on top of the formed replica structure. By forming a one-dimensional (1D) plasmonic grating with a periodicity of approximately 700 nm, a reflectance spectral dip was experimentally observed in the visible light region, from 600 to 700 nm, with increasing incident angle from 45° to 60°. This dip can be associated with surface plasmon resonance (SPR) wave excitation, which is coupled with the diffraction order m = - 2. The calculations of reflectance spectra simulation using the rigorous coupled wave analysis (RCWA) method have also been carried out, resulting in the appearance of an SPR dip in the range of 600-700 nm, for incident angles in the range of 45°-65°, which agrees with the experimental results. Interestingly, these RSR-PGs show richer plasmon characteristics than the sine-wave-shaped plasmonic gratings. The experimental and spectral simulation results revealed two different plasmonic excitation modes: long-range SPR and quasi-localized SPR (LSPR). While the long-range SPR was formed above the ridge sections along the grating structure surface, the quasi-localized SPR was locally formed inside the groove. In addition, for RSR-PGs with a narrow groove section, the long-range SPR seems to be coupled with the periodic structure of the grating, resulting in the appearance of plasmonic lattice surface resonance (LSR) that is indicated by a narrower plasmon resonance dip. These characteristics are quite different from those found in the sine wave-shaped plasmonic gratings. The present results may thus provide better insights for understanding the plasmon excitations in this type of rectangular plasmonic grating and might be useful for designing their structure for certain practical applications.

Modification of plasmonic properties in several transition metal-doped graphene studied by the first principles method.

Graphene doped with different transition metal (TM) atoms, namely, Co, Ni, Cu, Zn, and Au, have been investigated through first-principles calculations. The TM atom forms a substitutional defect, replacing one carbon atom in the graphene basal plane, which considerably can be obtained through wet or dry chemical processes as reported elsewhere. The calculation results showed that TM atom substitution leads to the opening of a band gap and the emergence of mid-gap states with the Fermi energy in the middle of it. The effects on optical properties were seen from the calculated optical absorption and Electron Energy Loss Spectroscopy (EELS) spectra. Two EELS bands are seen in the far UV region corresponding to the π and (π + σ) plasmons but the influence of the substituted TM effects on the plasmon frequency is small. On the other hand, as the Fermi energy level appears in the middle of the mid-gap state band while the real part of its dielectric permittivity at low photon energy is negative, these TM-doped graphene have a metal-like characteristic. Hence, plasmon wave excitation can be expected at the THz region which is dependent on the dopant TM atom. The plasmon excitation in these TM-doped graphene is thus principally similar to the plasmonic excitation in pure graphene by electric or magnetic fields, where the Fermi energy level is shifted from the graphene Dirac point leading to the possibility of an intraband transition.

Improving caregiver preparedness in the care transition of stroke patients: a scoping review.

Stroke is a life-threatening condition caused by the rupture of a brain blood vessel, potentially causing brain damage within minutes, severe disability, and even death. After initial hospitalization and rehabilitation, most stroke survivors return home, relying on their family members as caregivers for activities of daily living and treatments. This scoping review aimed to evaluate caregivers' readiness for transitioning stroke patient care to a home environment. We conducted a comprehensive search on Scopus, PubMed, ScienceDirect, and Google Scholar databases, followed by a secondary search to identify articles based on predefined criteria. A total of 14 articles were synthesized, leading to a series of findings: (1) there is a need to assess and identify caregiver needs, (2) the process needs relevant information, (3) caregivers are involved in the care process until the patients are discharged from the hospital, and (4) there is a need to offer support to caregivers. These results indicate that implementing strategies to enhance caregiver preparedness is crucial for the effective home-based care of stroke survivors.

Indonesia COVID-19 cases report using Linked Open Data.

Coronavirus disease is a worldwide pandemic. The need for accurate data and information become an important thing in this pandemic situation. In Indonesia, the government provides an official website for displaying COVID-19 spread statistics. However, the data provided does not follow the 5-star open data. As a result, the data is not reusable and integrated easily into another dataset and application. In this paper, we proposed an RDF vocabulary for presenting COVID-19 data in Indonesia. In addition, two queries are presented as an example for using our vocabulary and dataset as part of Linked Open data movement.

The application of K-means clustering for province clustering in Indonesia of the risk of the COVID-19 pandemic based on COVID-19 data.

This study was conducted with the aim to the clustering of provinces in Indonesia of the risk of the COVID-19 pandemic based on coronavirus disease 2019 (COVID-19) data. This clustering was based on the data obtained from the Indonesian COVID-19 Task Force (SATGAS COVID-19) on 19 April 2020. Provinces in Indonesia were grouped based on the data of confirmed, death, and recovered cases of COVID-19. This was performed using the K-Means Clustering method. Clustering generated 3 provincial groups. The results of the provincial clustering are expected to provide input to the government in making policies related to restrictions on community activities or other policies in overcoming the spread of COVID-19. Provincial Clustering based on the COVID-19 cases in Indonesia is an attempt to determine the closeness or similarity of a province based on confirmed, recovered, and death cases. Based on the results of this study, there are 3 clusters of provinces.

Exploring the Factor Structure of Environmental Attitudes Measure in a Sample of Indonesian College Students / Exploración de la estructura factorial de la Medida de Actitudes Ambientales en una muestra de estudiantes universitarios indonesios

Resumen El propósito de este estudio fue validar y evaluar la estructura factorial del instrumento de Medición de Actitudes hacia el Medio Ambiente mediante el análisis factorial y el modelo de Rasch politómico multidimensional. Participaron 595 estudiantes universitarios indonesios (293 hombres y 302 mujeres) con un rango de edad de 18-24 (edad media = 21,01, SD = 1,65). Los 22 ítems de la Medida de Actitudes Ambientales se administraron mediante encuestas en línea. Este estudio genera una estructura factorial tridimensional de actitudes ambientales en las muestras universitarias de Indonesia y respalda la base teórica de la escala original. Los resultados del análisis multidimensional del modelo politómico de Rasch muestran que las características psicométricas de este instrumento son excelentes y tienen una alta confiabilidad de separación, tanto por ítems como por persona. Los hallazgos de este estudio se pueden utilizar en investigaciones futuras para examinar la interrelación de las actitudes ambientales con otras variables en la muestra de Indonesia. También se discuten las limitaciones de este estudio.

Abstract The purpose of this study was to validate and evaluate the factor structure of the instrument for measuring attitudes towards the environment using factor analysis and multidimensional polytomous Rasch model. Participants in this study were 595 Indonesian university students (293 male and 302 female) with an age range of 18-24 (mean age = 21.01, SD = 1.65). The 22-items of Environmental Attitudes Measure were administered using online survey systems. This study generates a three-dimensional factor structure of environmental attitudes in the Indonesian college samples and supports the original scale's theoretical basis. The results of the multidimensional analysis of the polytomous Rasch model show that the psychometric characteristics of this instrument are excellent and have high separation reliability, both for items and person. The findings of this study can be used in future research to examine the interrelationship of environmental attitudes with other variables in the Indonesian sample. Limitations of this study are also discussed.

Revealing the charge carrier kinetics in perovskite solar cells affected by mesoscopic structures and defect states from simple transient photovoltage measurements.

This report shows that, by using simple transient photovoltage (TPV) measurements, we can reveal a significant correlation between the TPV decay characteristics and the performance of these perovskite solar cells. TPV decay seems to be composed of a rising part in a short interval after photoexcitation and a long decaying part that extends up to tens of milliseconds. These decay behaviors look different depending on the mesoscopic structures and the perovskite morphology formed therein, as seen from their Scanning Electron Microcopy images and X-ray diffraction patterns. The decay part can be fitted with a three-exponential decay, which reflects different kinetics of electrons in the perovskite/TiO2 layer. On the other hand, the rising part must be fit by a decay equation derived by employing the convolution theorem, where the rising part can be assigned to the electron transport process inside the perovskite layer and the decaying part can be assigned to electron back-transfer. The characteristics can be then understood by considering the effect of crystal defects and trap states in the perovskite grains and perovskite interface with its transport layer, which is TiO2 in this study. Although the TPV decay occurs in a time range much longer than the primary process of photoexcitation as commonly observed in transient photoluminescence spectroscopy, the processes involved in this TPV strongly correlates with the performance of these perovskite solar cells.

Community HIV Screening Among MSM in Three Indonesian Cities.

BACKGROUND: Indonesia lags behind its peers with regard to ART coverage of PLHIV. Insufficient HIV testing among MSM and other key affected populations remains a barrier to increasing ART coverage. OBJECTIVE: This report presents the results of the first government-endorsed implementation research study of a community screening approach to increasing the rate of HIV testing among MSM in three cities. METHODS: All new MSM outreach contacts meeting eligibility criteria during March-June 2017 were included in the study. HIV testing at a government health facility, which is required to qualify for government-supported ART, was advised for all new contacts. Men refusing formal testing were offered an HIV screening test in a community setting using a saliva-based rapid test and advised to get a confirmatory test at a health facility. All outreach contacts and testing activities were recorded on individual client records. RESULTS: Of 1,149 eligible MSM, 27% were willing to be referred to receive HIV testing at a health facility, among which 83% were confirmed to have been tested. Of the 838 study subjects refusing health facility testing, 38% accepted community screening. The screening positivity rate was 14.8%. Only 38% of men with reactive screening tests received a confirmatory test at a health facility, along with 8% of those with non-reactive tests. CONCLUSION: While community screening resulted in more MSM knowing their HIV status, reluctance to avail government health facility-based services, or indeed to be tested at all, must be addressed if community screening is to accelerate progress in getting HIV-positive MSM onto treatment.

The Impact of Concave Coastline on Rainfall Offshore Distribution over Indonesian Maritime Continent.

Indonesian Maritime Continent has the second longest coastline in the world, but the characteristics of offshore rainfall and its relation to coastline type are not clearly understood. As a region with eighty percent being an ocean, knowledge of offshore rainfall is important to support activity over oceans. This study investigates the climatology of offshore rainfall based on TRMM 3B42 composite during 1998-2015 and its dynamical atmosphere which induces high rainfall intensity using WRF-ARW. The result shows that concave coastline drives the increasing rainfall over ocean with Cenderawasih Bay (widest concave coastline) having the highest rainfall offshore intensity (16.5 mm per day) over Indonesian Maritime Continent. Monthly peak offshore rainfall over concave coastline is related to direction of concave coastline and peak of diurnal cycle influenced by the shifting of low level convergence. Concave coastline facing the north has peak during northwesterly monsoonal flow (March), while concave coastline facing the east has peak during easterly monsoonal flow (July). Low level convergence zone shifts from inland during daytime to ocean during nighttime. Due to shape of concave coastline, land breeze strengthens low level convergence and supports merging rainfall over ocean during nighttime. Rainfall propagating from the area around inland to ocean is approximately 5.4 m/s over Cenderawasih Bay and 4.1 m/s over Tolo Bay. Merger rainfall and low level convergence are playing role in increasing offshore rainfall over concave coastline.