Evaluation of surveillance methods for monitoring house fly abundance and activity on large commercial dairy operations.

Relative house fly, Musca domestica L., activity at three large dairies in central California was monitored during the peak fly activity period from June to August 2005 by using spot cards, fly tapes, bait traps, and Alsynite traps. Counts for all monitoring methods were significantly related at two of three dairies; with spot card counts significantly related to fly tape counts recorded the same week, and both spot card counts and fly tape counts significantly related to bait trap counts 1-2 wk later. Mean fly counts differed significantly between dairies, but a significant interaction between dairies sampled and monitoring methods used demonstrates that between-dairy comparisons are unwise. Estimate precision was determined by the coefficient of variability (CV) (or SE/mean). Using a CV = 0.15 as a desired level of estimate precision and assuming an integrate pest management (IPM) action threshold near the peak house fly activity measured by each monitoring method, house fly monitoring at a large dairy would require 12 spot cards placed in midafternoon shaded fly resting sites near cattle or seven bait traps placed in open areas near cattle. Software (FlySpotter; http://ucanr.org/ sites/FlySpotter/download/) using computer vision technology was developed to count fly spots on a scanned image of a spot card to dramatically reduce time invested in monitoring house flies. Counts provided by the FlySpotter software were highly correlated to visual counts. The use of spot cards for monitoring house flies is recommended for dairy IPM programs.

Association of minimum inhibitory concentration cluster patterns with dairy management practices for environmental bacteria isolated from bulk tank milk.

Environmental bacteria have emerged over the past few years to become significant causes of mastitis. Bacteria in this group are often reported by practicing veterinarians to be increasingly resistant to intramammary therapy and responsible for elevated bulk tank somatic cell counts. The purpose of this study was to determine the extent of association of the minimum inhibitory concentrations for selected antimicrobial agents with environmental bacteria isolated from bulk tank milk on California dairies and their housing facilities, husbandry practices, and antimicrobic-use strategies. Bulk tank milk samples were collected from 2 dairy cooperatives that had their milk cultured at the Milk Quality Laboratory, University of California Davis, Veterinary Medicine Teaching and Research Center in Tulare, CA. Samples were collected from July 2001 through March 2002 on 88 d; and 404 environmental bacteria isolated from 93 dairies were found. Minimum inhibitory concentrations were determined on 337 of the isolates for 10 antimicrobial agents. Cluster analysis was performed on the minimum inhibitory concentration values for each organism, and 4 antimicrobial clusters with varying degrees of resistance were found.A 69-question survey questionnaire was completed on-farm for 49 of the 73 dairies that had at least 3 environmental bacterial isolates. The questionnaire sought information on housing facilities, milking management, mastitis prevention, antimicrobial usage strategies, and owner/veterinary involvement in disease control and prevention. Multinomial logistic regression analysis found significant associations between the antimicrobial agent-resistance cluster groups and some of the housing and bedding practices, failure to dry udders before milking, and antimicrobial treatment of nonmastitis conditions. No association was noted for antimicrobial agent treatment of mastitis and the resistance cluster patterns.

Effect of timing of first clinical mastitis occurrence on lactational and reproductive performance of Holstein dairy cows.

Objectives of this study were to determine the influence of timing of first clinical mastitis case occurrence on lactational and reproductive performance in high producing lactating dairy cows during the first 320 days in milk (DIM). Holstein cows, 1001, from two commercial dairy farms in California were retrospectively divided into four treatment groups according to timing of first clinical mastitis case caused by environmental pathogens: control with no recorded clinical cases of mastitis (C; n=501); first clinical mastitis prior to first postpartum AI (MG1; n=250); first clinical mastitis between first postpartum AI and pregnancy diagnosis (MG2; n=147); and first clinical mastitis after diagnosed pregnant (MG3; n=103). Clinical cases of mastitis were identified at every milking by the herd personnel based on abnormal milk or swelling of the mammary gland. A fore sample of milk was aseptically collected from every clinical case for microbiological culture. Mastitis decreased yields of milk, 3.5% fat-corrected milk, and milk components, but the effect was only observed for MG1 and MG2. Cows in the control group had lower linear somatic cell count (SCC) score throughout the lactation. Culling was increased by mastitis, and cows in the mastitis groups left the study earlier than controls. Conception rate at first postpartum AI and pregnancy rate at the end of the study were both decreased by mastitis prior to or after first AI, and MG1 and MG2 cows had extended days open. Furthermore, cows experiencing mastitis during lactation had a higher incidence of abortions. The negative effects of mastitis on reproduction were observed regardless of clinical case being caused by either Gram positive or negative bacteria. Mastitis either prior to or after first postpartum AI impairs lactation performance, increases culling, and decreases reproductive efficiency in high producing Holstein dairy cows.

Effect of yeast culture and Aspergillus oryzae extract on milk yield in a commercial dairy herd.

The objective of this study was to examine the effects of an Aspergillus oryzae extract in combination with a yeast culture (Saccharomyces cerevisiae) on milk yield and composition, rectal temperatures, and rumen parameters in a commercial dairy herd. Pluriparous Holstein cows (n = 521) in early lactation were assigned to a 130-d trial from May to September 1992. Treatments were control (no additive) and yeast culture (56 g/d) plus A. oryzae (3 g/d). Both groups were fed a TMR composed of alfalfa silage, alfalfa hay, corn silage, rolled barley, rolled corn, grass screenings, whole cottonseed, wet citrus pulp, molasses supplement, protein pellet, and minerals. No differences were detected in actual milk, 3.5% FCM, or percentages of milk fat or protein. Percentages of lactose and SNF were lower for the group fed yeast culture plus A. oryzae. Rectal temperatures were lower for cows fed yeast culture plus A. oryzae for 7 of 17 readings made during the summer. The greatest difference occurred during hotter months.

Influence of feeding Aspergillus oryzae fermentation extract on the milk yields, eating patterns, and body temperatures of lactating cows.

Trials were conducted to evaluate effects of a fermentation extract of Aspergillus oryzae (AO) on milk production and composition, diet digestibility, and rectal temperature changes in lactating dairy cows. Treatments were incorporated as a top dressing at the morning feeding and consisted of control (90 g/d of ground sorghum) or AO (3 g of culture + 87 g of ground sorghum daily). Twenty-four mid-lactation Holstein cows were paired for production in Lactation Trial 1 (LT-1). In Lactation Trial 2 (LT-2), 46 cows (20 primiparous and 26 multiparous) in early lactation were used. Trials lasted 12 wk. In LT-1, AO supplementation increased milk yields only at 2 (P less than .05) and 8 wk (P less than .10) of treatment. Rectal temperatures were lower (P less than .05) for cows fed AO for 4 of 10 readings made during summer. Supplementation of AO culture in LT-2 (early lactation cows) increased milk production and feed efficiency (P less than .05). Inner ear temperatures tended to be lower (P less than .11) for cows fed AO. Digestion trials, conducted at the end of lactation trials, used Cr2O3 as an indigestible marker. In Digestion Trial 1, digestibilities were not significantly (P greater than .10) affected by AO supplementation. However, in Digestion Trial 2, AO increased (P less than .05) digestibilities of DM, OM, CP, NDF, and ADF. Length and number of meals were not affected (P greater than .10) by feeding AO. In summary, milk yields, efficiency of milk production, and nutrient digestibilities were higher for early lactation cows fed a high-concentrate diet supplemented with 3 g of AO/d. Mid-lactation cows fed a lower-energy diet were less responsive to AO than early lactation cows, though similar trends were shown.

Influence of dietary protein concentration and degradability on performance of lactating cows during hot environmental temperatures.

To test effects of protein concentrations and degradability, 60 lactating Holstein cows in midlactation were offered the following diets during three trials between May and October in Tucson, AZ: high protein (18.4%), high degradability; high protein (18.5%), medium degradability; medium protein (16.1%), high degradability; and medium protein (16.1%), medium degradability. Diets comprised 39% alfalfa hay, 12% cottonseed hulls, 10% whole cottonseed, and 39% concentrate (DM) and concentrates contained 60, 40, 57, and 40% degradability, respectively, as determined by ficin assay. Rectal and ambient temperatures suggested that cows were under moderate to intense heat stress, as did group water intakes, which were increased about 15% by high degradability. Milk yields (3.5% FCM) and persistencies were lower for the high protein, high degradability diet than for all others. Mean DM intakes across treatments were quite high but were lower on high than medium protein; whereas ruminal ammonia and blood serum urea were higher on high protein. Milk composition, ruminal VFA, serum glucose, thyroxine, triiodothyronine, and cortisol were not affected by treatment.

Influence of protein percentage and degradability on performance of lactating cows during moderate temperature.

Sixty high producing Holstein cows (15/treatment) averaging 157 d postpartum were offered the following diets: high protein (18.5%), high rumen degradability (60%); high protein (18.0%), medium degradability (41%); medium protein (15.4%), high degradability (61%); medium protein (15.0%), medium degradability (46%). All diets contained 32% corn silage, 15% alfalfa haylage, and 10% whole cottonseed. Degradabilities were determined by the ficin method and were lowered by replacing soybean meal with meat and bone meal and brewers dried grains. Cows were milked three times daily and the study was conducted from May 11 to June 23 in Provo, UT during ascending day temperatures but cool nights. Milk yields were not different for the respective treatments (36.9, 35.4, 34.8, and 36.7 kg/d), but milk fat was higher at high protein degradability: 3.11, 2.89, 3.04, 2.78%, respectively, resulting in a tendency toward higher FCM. Lower acetate and propionate and acetate:propionate ratios were observed for medium degradability diets and were consistent with lower milk fat. Rumen NH3, butyrate, valerate, isovalerate, 2-methyl butyrate, and blood urea N were higher for cows on high protein diets but were not affected by degradability. Serum glucose and cortisol were not significantly affected by protein treatment, but serum triiodothyronine was lower on high protein; the interaction effect for thyroxine was significant.

Nutritive value of high moisture alfalfa hay preserved with urea.

Urea (2 and 4%) was added to high moisture hays and compared with untreated wet or dry hays. Means for bale temperatures (degree C) for 1 wk postharvest were highest for wet control (41.2), lowest for dry control (31.4), and intermediate for urea treatments (36.7). By 4 wk postharvest, all temperatures were equal. Protection from molding was most effective for 4% urea, and 2% urea was superior to wet control. By 4 mo postharvest, CP for low urea had decreased due to NH3 loss, and CP for wet control had increased due to DM loss, so they were not different. Initially, ADF was similar for the hays, but after 4 mo storage the wet control was highest (41.7%), the dry control lowest (36.0%), and urea treatments intermediate. Lignin and NDF followed similar trends. In vitro DM digestibilities were highest for the wet control at harvest but lowest at 120 d with high urea and dry control greater than low urea. Feed intakes and milk yields in lactating cows fed the hays were not different. These data show that addition of 2 or 4% urea improved quality of hay baled at about 25% moisture; high urea was more effective than low urea.