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1.
Int J Exerc Sci ; 15(5): 125-141, 2022.
Article in English | MEDLINE | ID: mdl-36896451

ABSTRACT

Metabolic syndrome (MetS) is typically diagnosed in adults; however, MetS risk factors are growing in prevalence during youth and young adulthood. Though the transition from high school to college is associated with adverse changes in lifestyle behaviors that may contribute to MetS risk factor development, the relationship between pre-college MetS risk status and transition-related behavior change is unknown. This prospective study aimed to describe the relationship between pre-college MetS risk status and transition-related behavior change trajectories in college-bound students. Moreover, it aimed to assess the feasibility of the study design, including acceptability to both participants and investigators, prior to implementation in a larger sample. Participants (n = 21, 18.3 ± 0.3 y/o) were assessed for MetS risk factors during their last semester of high school. Self-report behavioral data on dietary habits, physical activity, sleep, stress, and alcohol consumption were collected at baseline and during the fall and spring semesters of the first year of college. Linear mixed models revealed drastic increases in alcohol consumption (ß11 = 0.39, p < 0.001) and apparent decreases in moderate-vigorous physical activity (ß11 = -0.15, p = 0.185) during the college transition. Furthermore, 47.6% of students had ≥ 1 MetS risk factor at baseline and those with a greater number of risk factors experienced a more severe alcohol-related behavior change trajectory (ß11 = 0.29, p < 0.050). These findings highlight the importance of primordial prevention strategies against early MetS risk development, given the potential relationship with future behavioral trajectories. Future research should aim to further characterize this relationship using comprehensive, longitudinal measures that span the college transition in larger, more diverse samples.

2.
Med Sci Sports Exerc ; 53(5): 951-959, 2021 05 01.
Article in English | MEDLINE | ID: mdl-33170820

ABSTRACT

PURPOSE: The aims of this project were twofold: 1) to assess the concurrent validity of raw accelerometer outputs with ground reaction forces (GRF) and loading rates (LR) calculated from force plate across a range of simulated habitual PA and 2) to identify the optimal wear site among the ankle, hip, and wrist with the strongest relationships between accelerometer and force plate and/or skeletal outcomes. METHODS: Thirty healthy young adults (23.0 ± 4.5 yr, 50% female) wore a triaxial accelerometer at the right ankle, hip, and wrist while performing eight trials of walking, jogging, running, low box drops, and high box drops over an in-ground force plate. Repeated-measures correlations and linear mixed models were used to assess concurrent validity of accelerometer and force plate outcomes across wear sites. RESULTS: Strong repeated-measures associations were observed between peak hip resultant acceleration and resultant LR (rrm 1169 = 0.74, P < 0.001, 95% confidence interval = 0.718, 0.769) and peak hip resultant accelerations and resultant GRF (rrm 1169 = 0.69, P < 0.001, 95% confidence interval = 0.660, 0.720) when data were combined across activities. By contrast, small to moderate associations were seen between ankle-based outcomes and corresponding GRF and LR during walking and jogging (rrm 209 = 0.17-0.34, all P < 0.001). No significant associations were seen with wrist-based outcomes during any activity. In addition, linear mixed models suggested that 24%-50% of the variability in peak GRF and LR could be attributed to measured accelerations at the hip. CONCLUSION: Peak accelerations measured at the hip were identified as the strongest proxies for skeletal loading assessed via force plate.


Subject(s)
Acceleration , Accelerometry/instrumentation , Bone and Bones/physiology , Running/physiology , Walking/physiology , Actigraphy , Adult , Biomechanical Phenomena/physiology , Confidence Intervals , Female , Humans , Jogging/physiology , Linear Models , Male , Reproducibility of Results , Young Adult
3.
PLoS One ; 15(11): e0242250, 2020.
Article in English | MEDLINE | ID: mdl-33253217

ABSTRACT

RATIONALE: The beta-O-linkage of N-acetylglucosamine (i.e., O-GlcNAc) to proteins is a pro-adaptive response to cellular insults. To this end, increased protein O-GlcNAcylation improves short-term survival of cardiomyocytes subjected to acute injury. This observation has been repeated by multiple groups and in multiple models; however, whether increased protein O-GlcNAcylation plays a beneficial role in more chronic settings remains an open question. OBJECTIVE: Here, we queried whether increasing levels of cardiac protein O-GlcNAcylation would be beneficial during infarct-induced heart failure. METHODS AND RESULTS: To achieve increased protein O-GlcNAcylation, we targeted Oga, the gene responsible for removing O-GlcNAc from proteins. Here, we generated mice with cardiomyocyte-restricted, tamoxifen-inducible haploinsufficient Oga gene. In the absence of infarction, we observed a slight reduction in ejection fraction in Oga deficient mice. Overall, Oga reduction had no major impact on ventricular function. In additional cohorts, mice of both sexes and both genotypes were subjected to infarct-induced heart failure and followed for up to four weeks, during which time cardiac function was assessed via echocardiography. Contrary to our prediction, the Oga deficient mice exhibited exacerbated-not improved-cardiac function at one week following infarction. When the observation was extended to 4 wk post-MI, this acute exacerbation was lost. CONCLUSIONS: The present findings, coupled with our previous work, suggest that altering the ability of cardiomyocytes to either add or remove O-GlcNAc modifications to proteins exacerbates early infarct-induced heart failure. We speculate that more nuanced approaches to regulating O-GlcNAcylation are needed to understand its role-and, in particular, the possibility of cycling, in the pathophysiology of the failing heart.


Subject(s)
Myocardial Infarction/pathology , Myocardium/enzymology , N-Acetylglucosaminyltransferases/genetics , Ventricular Dysfunction/etiology , Animals , Echocardiography , Female , Glycosylation , Haploinsufficiency , Heart/physiology , Heart Failure/metabolism , Heart Failure/pathology , Humans , Male , Mice , Mice, Knockout , Middle Aged , Myocardial Infarction/complications , Myocardium/metabolism , Myocardium/pathology , N-Acetylglucosaminyltransferases/deficiency , N-Acetylglucosaminyltransferases/metabolism , Tamoxifen/pharmacology , Up-Regulation , Ventricular Function/drug effects
4.
Basic Res Cardiol ; 114(4): 28, 2019 05 31.
Article in English | MEDLINE | ID: mdl-31152247

ABSTRACT

Several post-translational modifications figure prominently in ventricular remodeling. The beta-O-linkage of N-acetylglucosamine (O-GlcNAc) to proteins has emerged as an important signal in the cardiovascular system. Although there are limited insights about the regulation of the biosynthetic pathway that gives rise to the O-GlcNAc post-translational modification, much remains to be elucidated regarding the enzymes, such as O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), which regulate the presence/absence of O-GlcNAcylation. Recently, we showed that the transcription factor, E2F1, could negatively regulate OGT and OGA expression in vitro. The present study sought to determine whether E2f1 deletion would improve post-infarct ventricular function by de-repressing expression of OGT and OGA. Male and female mice were subjected to non-reperfused myocardial infarction (MI) and followed for 1 or 4 week. MI significantly increased E2F1 expression. Deletion of E2f1 alone was not sufficient to alter OGT or OGA expression in a naïve setting. Cardiac dysfunction was significantly attenuated at 1-week post-MI in E2f1-ablated mice. During chronic heart failure, E2f1 deletion also attenuated cardiac dysfunction. Despite the improvement in function, OGT and OGA expression was not normalized and protein O-GlcNAcyltion was not changed at 1-week post-MI. OGA expression was significantly upregulated at 4-week post-MI but overall protein O-GlcNAcylation was not changed. As an alternative explanation, we also performed guided transcriptional profiling of predicted targets of E2F1, which indicated potential differences in cardiac metabolism, angiogenesis, and apoptosis. E2f1 ablation increased heart size and preserved remote zone capillary density at 1-week post-MI. During chronic heart failure, cardiomyocytes in the remote zone of E2f1-deleted hearts were larger than wildtype. These data indicate that, overall, E2f1 exerts a deleterious effect on ventricular remodeling. Thus, E2f1 deletion improves ventricular remodeling with limited impact on enzymes regulating O-GlcNAcylation.


Subject(s)
E2F1 Transcription Factor/deficiency , Myocardial Infarction/metabolism , Myocardium/metabolism , Ventricular Function, Left , Ventricular Remodeling , Animals , Capillaries/metabolism , Capillaries/pathology , Coronary Vessels/metabolism , Coronary Vessels/pathology , Disease Models, Animal , E2F1 Transcription Factor/genetics , Female , Gene Deletion , Glycosylation , Male , Mice, Inbred C57BL , Mice, Knockout , Myocardial Infarction/genetics , Myocardial Infarction/pathology , Myocardial Infarction/physiopathology , Myocardium/pathology , N-Acetylglucosaminyltransferases/metabolism , beta-N-Acetylhexosaminidases/metabolism
5.
PLoS One ; 5(2): e9071, 2010 Feb 05.
Article in English | MEDLINE | ID: mdl-20140205

ABSTRACT

BACKGROUND: Helicobacter pylori is the etiologic agent of common gastritis and a risk factor for gastric cancer. It is also one of the richest sources of Type II restriction-modification (R-M) systems in microorganisms. PRINCIPAL FINDINGS: We have cloned, expressed and purified a new restriction endonuclease HpyAV from H. pylori strain 26695. We determined the HpyAV DNA recognition sequence and cleavage site as CCTTC 6/5. In addition, we found that HpyAV has a unique metal ion requirement: its cleavage activity is higher with transition metal ions than in Mg(++). The special metal ion requirement of HpyAV can be attributed to the presence of a HNH catalytic site similar to ColE9 nuclease instead of the canonical PD-X-D/EXK catalytic site found in many other REases. Site-directed mutagenesis was carried out to verify the catalytic residues of HpyAV. Mutation of the conserved metal-binding Asn311 and His320 to alanine eliminated cleavage activity. HpyAV variant H295A displayed approximately 1% of wt activity. CONCLUSIONS/SIGNIFICANCE: Some HNH-type endonucleases have unique metal ion cofactor requirement for optimal activities. Homology modeling and site-directed mutagenesis confirmed that HpyAV is a member of the HNH nuclease family. The identification of catalytic residues in HpyAV paved the way for further engineering of the metal binding site. A survey of sequenced microbial genomes uncovered 10 putative R-M systems that show high sequence similarity to the HpyAV system, suggesting lateral transfer of a prototypic HpyAV-like R-M system among these microorganisms.


Subject(s)
Bacterial Proteins/metabolism , DNA Restriction Enzymes/metabolism , Helicobacter pylori/enzymology , Metals/metabolism , Amino Acid Sequence , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Base Sequence , Binding Sites/genetics , Catalytic Domain/genetics , Cations, Divalent/chemistry , Cations, Divalent/metabolism , Cobalt/chemistry , Cobalt/metabolism , DNA/genetics , DNA/metabolism , DNA Restriction Enzymes/chemistry , DNA Restriction Enzymes/genetics , DNA Restriction-Modification Enzymes/genetics , DNA Restriction-Modification Enzymes/metabolism , Helicobacter pylori/genetics , Manganese/chemistry , Manganese/metabolism , Metals/chemistry , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Nickel/chemistry , Nickel/metabolism , Protein Structure, Tertiary , Sequence Homology, Amino Acid
6.
Protein Expr Purif ; 58(1): 42-52, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18164625

ABSTRACT

BmrI (ACTGGG N5/N4) is one of the few metal-independent restriction endonucleases (REases) found in bacteria. The BmrI restriction-modification system was cloned by the methylase selection method, inverse PCR, and PCR. BmrI REase shows significant amino acid sequence identity to BfiI and a putative endonuclease MspBNCORF3798 from the sequenced Mesorhizobium sp. BNC1 genome. The EDTA-resistant BmrI REase was successfully over-expressed in a pre-modified E. coli strain from pET21a or pBAC-expIQ vectors. The recombinant BmrI REase shows strong promiscuous activity (star activity) in NEB buffers 1, 4, and an EDTA buffer. Star activity was diminished in buffers with 100-150 mM NaCl and 10 mM MgCl(2). His-tagged BmrI192, the N-terminal cleavage domain of BmrI, was expressed in E. coli and purified from inclusion bodies. The refolded BmrI192 protein possesses non-specific endonuclease activity. BmrI192 variants with a single Ser to Cys substitution (S76C or S90C) and BmrI200 (T200C) with a single Cys at the C-terminal end were also constructed and purified. BmrI200 digests both single-strand (ss) and double-strand (ds) DNA and the nuclease activity on ss DNA is at least 5-fold higher than that on ds DNA. The Cys-containing BmrI192 and BmrI200 nuclease variants may be useful for coupling to other DNA binding elements such as synthetic zinc fingers, thio-containing locked nucleic acids (LNA) or peptide nucleic acids (PNA).


Subject(s)
Deoxyribonucleases, Type II Site-Specific , Amino Acid Sequence , Bacillus megaterium/enzymology , Cloning, Molecular , DNA Cleavage , Deoxyribonucleases, Type II Site-Specific/chemistry , Deoxyribonucleases, Type II Site-Specific/genetics , Deoxyribonucleases, Type II Site-Specific/isolation & purification , Deoxyribonucleases, Type II Site-Specific/metabolism , Escherichia coli/genetics , Gene Expression , Molecular Sequence Data , Plasmids , Protein Structure, Tertiary , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/isolation & purification , Recombinant Fusion Proteins/metabolism , SOS Response, Genetics , Temperature
7.
Protein Eng Des Sel ; 20(10): 497-504, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17951612

ABSTRACT

A cleavage-deficient variant of NotI restriction endonuclease (GCGGCCGC) was isolated by random mutagenesis of the notIR gene. The NotI variant D160N was shown to bind DNA and protect plasmid DNA from EagI (CGGCCG) and NotI digestions. The EDTA-resistant BmrI restriction endonuclease cleaves DNA sequence ACTGGG N5/N4. The N-terminal cleavage domain of BmrI (residues 1-198) with non-specific nuclease activity was fused to the NotI variant D160N with a short linker. The engineered chimeric endonuclease (CH-endonuclease) recognizes NotI sites specifically in the presence of high salt (100-150 mM NaCl) and divalent cations Mg++ or Ca++. In contrast to wild-type NotI, which cuts within its recognition sequence, BmrI198-NotI (D160N) cleaves DNA outside of NotI sites, resulting in deletion of the NotI site and the adjacent sequences. The fusion of the BmrI cleavage domain to cleavage-deficient variants of Type II restriction enzymes to generate novel cleavage sites will provide useful tools for DNA manipulation.


Subject(s)
Deoxyribonucleases, Type II Site-Specific/genetics , Deoxyribonucleases, Type II Site-Specific/metabolism , Drug Design , Mutant Chimeric Proteins/genetics , Mutant Chimeric Proteins/metabolism , Base Sequence , DNA Cleavage , Electrophoresis, Polyacrylamide Gel , Molecular Sequence Data , Protein Structure, Tertiary , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Sequence Deletion , Substrate Specificity , Temperature
8.
Microbiology (Reading) ; 151(Pt 4): 1301-1311, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15817797

ABSTRACT

The serotype F phage Phi42 of Staphylococcus aureus is a triple-converting bacteriophage that encodes the staphylokinase gene (sak) and the enterotoxin A gene (entA). Lysogeny results in loss of expression of the chromosomal beta-haemolysin gene (hlb) (negative conversion), the expression of staphylokinase and enterotoxin A (positive conversion), and the acquisition of resistance to lysis by all 23 phages of the International Basic Set (IBS) of S. aureus typing phages. Until this study, the basis of Phi42 resistance to lysis by exogenous phages was unknown. The authors report here that phage Phi42 encodes a restriction-modification (R-M) system, termed Sau42I, adjacent to and in the same orientation to the phage integrase gene int. The genes encoding Sau42I were cloned and sequenced, and found to consist of two overlapping reading frames, ORF S (specificity) and ORF RM (restriction-modification), in the same orientation. The ORFs share a high degree of DNA and amino acid sequence homology with the previously characterized BcgI R-M system of Bacillus coagulans. Expression of the cloned Sau42I ORF S and ORF RM in S. aureus 80CR3 transformants from a plasmid vector conferred resistance to lysis by all 23 IBS phages. Similarly, transformants of S. aureus RN4220 harbouring recombinant plasmids containing both ORFs were resistant to lysis by the IBS typing phages. However, transformants harbouring plasmids encoding either ORF S or ORF RM were susceptible to lysis by the IBS phages, and they had the same phage-susceptibility pattern as the respective parental isolates. In vitro analysis of crude and partially purified extracts of S. aureus transformants harbouring both the Phi42 ORF S and ORF RM genes indicated that Sau42I has endonuclease activity and requires co-factors Mg(2+) and S-adenosylmethionine in order to function, and activity is optimized at pH 8, although the precise recognition sequence has yet to be determined. The findings of this study confirm that Phi42 is a quadruple-converting phage, believed to be the first described for S. aureus, and show that it encodes a novel R-M system termed Sau42I.


Subject(s)
DNA Restriction-Modification Enzymes/genetics , DNA Restriction-Modification Enzymes/metabolism , Staphylococcus Phages/genetics , Staphylococcus Phages/metabolism , Amino Acid Sequence , Chromosome Mapping , Cloning, Molecular , Escherichia coli/genetics , Genes, Viral , Lysogeny , Molecular Sequence Data , Open Reading Frames , Phenotype , Sequence Homology, Amino Acid , Staphylococcus Phages/classification , Staphylococcus aureus/virology
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