ABSTRACT
The membrane ATPase (EC 3.6.1.3) of Bacillus cereus was solubilized by a 'shock-wash' process and purified. The non-specific phosphatase contaminant was separated by glycerol density gradient centrifugation. The optimum temperature was 39.5 degrees C and the pH optimum at 7.5. On SDS-polyacrylamide gel electrophoresis two classes of subunits were observed in equal proportions with molecular weights of 70 K and 83 K. The effect of various compounds on the enzymatic activity was studied. The enzyme was insensitive to NaN3, oligomycin and to divalent cations, but was inhibited by citrate and oxalate.
Subject(s)
Adenosine Triphosphatases/isolation & purification , Bacillus cereus/enzymology , Adenosine Triphosphatases/antagonists & inhibitors , Adenosine Triphosphatases/chemistry , Bacillus cereus/drug effects , Bacillus cereus/growth & development , Cell Membrane/enzymology , Citrates/pharmacology , Citric Acid , Hydrogen-Ion Concentration , Molecular Weight , Protein Conformation , Solubility , TemperatureABSTRACT
The effect of exogenous vitamin E (2.1 mg/100 ml) was determined on the preservation of red blood cell integrity (PCV and RBC counts) during long-term storage. Cells from healthy human blood donors, normal rats and rats raised on a vitamin E-deficient diet were stored in CE medium at 4 degrees C for 40-60 days. The addition of vitamin E to the CE medium protected normal rat RBC and those from vitamin E-deficient animals to even a larger extent. Neither the PVC nor RBC count in human blood changed during storage for up to 60 days and no effect of exogenous vitamin E was demonstrable.
Subject(s)
Blood Preservation/methods , Erythrocytes/drug effects , Vitamin E Deficiency/blood , Vitamin E/pharmacology , Animals , Culture Media/analysis , Erythrocyte Count , Hematocrit , Humans , RatsABSTRACT
The effect of exogenous vitamin E (2.1 mg/100 ml) was determined on the preservation of red blood cell integrity (PCV and RBC counts) during long-term storage. Cells from healthy human blood donors, normal rats and rats raised on a vitamin E-deficient diet were stored in CE medium at 4-C for 40-60 days. The addition of vitamin E to the CE medium protected normal rat RBC and those from vitamin E-deficient animals to even a larger extent. Neither the PVC nor RBC count in human blood changed during storage for up to 60 days and no effect of exogenous vitamin E was demonstrable