Long-term persistence and recycling of Bacillus thuringiensis israelensis spores in wetlands sprayed for mosquito control.

Bacillus thuringiensis subsp. israelensis (Bti) is the main larvicide used to control mosquitoes worldwide. Although there is accumulating evidence of Bti having environmental effects on non-target fauna, relatively few field studies have documented the fate of Bti spores in the environment. Spore density was quantified over a 6-yr period (2012-2017) in Mediterranean marshes sprayed with Vectobac 12AS (32 ITU/ha) since 2006 to reduce the nuisance caused by Aedes caspius. Bti spores were naturally found in all habitat types. Spore density expressed as colony-forming units per gram of soil (CFU g-1) increased significantly at treated sites by a factor of 22 to 500 times relative to control sites, with mean values of 7730 CFU g-1 in halophilous scrubs, 38,000 in reed beds, 49,000 in bulrush beds and 50 000 in rush beds. Spore density varied little in the first months after the spraying season (April-October), but increased sharply in spring, just before the annual launch of mosquito control. Considering that Bti is an insect pathogen that cannot proliferate without a suitable insect host, this unexpected recrudescence in spring could be related to the warming of water that triggers activity and development of benthic organisms such as chironomids, which may contribute to Bti proliferation by ingesting accumulated spores at the surface of sediments. While spore density tends to decrease over time, presumably during the summer period as a result of increased UV exposure, three to four years were necessary for spore density to return to normal levels after mosquito-control interruption. This study is important because it demonstrates that environmental effects of mosquito-control using Bti can far exceed the short period of Bti efficacy against lentic mosquitoes. Considering that Bti is a microbial agent, these long-term effects should be addressed at multiple levels of ecosystem organization from a one-health perspective.

Increasing crop heterogeneity enhances multitrophic diversity across agricultural regions.

Agricultural landscape homogenization has detrimental effects on biodiversity and key ecosystem services. Increasing agricultural landscape heterogeneity by increasing seminatural cover can help to mitigate biodiversity loss. However, the amount of seminatural cover is generally low and difficult to increase in many intensively managed agricultural landscapes. We hypothesized that increasing the heterogeneity of the crop mosaic itself (hereafter "crop heterogeneity") can also have positive effects on biodiversity. In 8 contrasting regions of Europe and North America, we selected 435 landscapes along independent gradients of crop diversity and mean field size. Within each landscape, we selected 3 sampling sites in 1, 2, or 3 crop types. We sampled 7 taxa (plants, bees, butterflies, hoverflies, carabids, spiders, and birds) and calculated a synthetic index of multitrophic diversity at the landscape level. Increasing crop heterogeneity was more beneficial for multitrophic diversity than increasing seminatural cover. For instance, the effect of decreasing mean field size from 5 to 2.8 ha was as strong as the effect of increasing seminatural cover from 0.5 to 11%. Decreasing mean field size benefited multitrophic diversity even in the absence of seminatural vegetation between fields. Increasing the number of crop types sampled had a positive effect on landscape-level multitrophic diversity. However, the effect of increasing crop diversity in the landscape surrounding fields sampled depended on the amount of seminatural cover. Our study provides large-scale, multitrophic, cross-regional evidence that increasing crop heterogeneity can be an effective way to increase biodiversity in agricultural landscapes without taking land out of agricultural production.

Mosquito Traps: An Innovative, Environmentally Friendly Technique to Control Mosquitoes.

We tested the use of mosquito traps as an alternative to spraying insecticide in Camargue (France) following the significant impacts observed on the non-target fauna through Bti persistence and trophic perturbations. In a village of 600 inhabitants, 16 Techno Bam traps emitting CO2 and using octenol lures were set from April to November 2016. Trap performance was estimated at 70% overall based on mosquitoes landing on human bait in areas with and without traps. The reduction of Ochlerotatus caspius and Oc. detritus, the two species targeted by Bti spraying, was, respectively, 74% and 98%. Traps were less efficient against Anopheles hyrcanus (46%), which was more attracted by lactic acid than octenol lures based on previous tests. Nearly 300,000 mosquitoes from nine species were captured, with large variations among traps, emphasizing that trap performance is also influenced by surrounding factors. Environmental impact, based on the proportion of non-target insects captured, was mostly limited to small chironomids attracted by street lights. The breeding success of a house martin colony was not significantly affected by trap use, in contrast to Bti spraying. Our experiment confirms that the deployment of mosquito traps can offer a cost-effective alternative to Bti spraying for protecting local populations from mosquito nuisance in sensitive natural areas.